Objective To investigate the effects of mechanical ventilation on lung pathology and concentration of proinflammatory cytokines in rats.Methods Forty-five healthy Sprague-Dawley rats were randomly divided into three groups with 15 rats each group,ie.a control group(No mechanical ventilation),a high tidal volume group(VT 34 mL/kg,RR 30 bpm) and low tidal volume group(VT 8 mL/kg,RR 60 bpm).Results There were enlarged alveolar spaces,interalveolar septum collapse,swollen and spotty hemorrhages,inflammatory cell infiltration in the ventilation groups,which was more serious in the high tidal volume group.Lung wet-to-dry weight ratio of high tidal volume group was significantly higher than that of other groups(both P lt;0.05).The concentrations in both macrophage-inflammatory protein-2(MIP-2)and tumor necrosis factor-α(TNF-α) of bronchoalveolar lavage fluid(BALF) and blood samples was significantly higher in two ventilation groups than the control group(P lt;0.05),which were higer in the high tidal volume group than in the low tidal volume group(P lt;0.05).Conclusions These results indicate that mechanical ventilation can cause lung injury,which is more serious in high tidal volume ventilation.And mechanical ventilation-induced lung injury can cause proinflammatory cytokines release,with different levels in bronchoalveolar lavage fluid and blood samples.
Citation: DONG Chunshan,XU Zhixin,XU Ruihao,CHEN Xuehong,CHEN Rong,JIANG Tao,MIAO Yuqing,WEN Hao. The effects of mechanical ventilation-induced lung injury on concentration of proinflammatory cytokines in rats. Chinese Journal of Respiratory and Critical Care Medicine, 2008, 08(2): 135-139. doi: Copy