Objective To investigate the effects of mechanical ventilation on lung pathology and concentration of proinflammatory cytokines in rats.Methods Forty-five healthy Sprague-Dawley rats were randomly divided into three groups with 15 rats each group,ie.a control group(No mechanical ventilation),a high tidal volume group(VT 34 mL/kg,RR 30 bpm) and low tidal volume group(VT 8 mL/kg,RR 60 bpm).Results There were enlarged alveolar spaces,interalveolar septum collapse,swollen and spotty hemorrhages,inflammatory cell infiltration in the ventilation groups,which was more serious in the high tidal volume group.Lung wet-to-dry weight ratio of high tidal volume group was significantly higher than that of other groups(both P lt;0.05).The concentrations in both macrophage-inflammatory protein-2(MIP-2)and tumor necrosis factor-α(TNF-α) of bronchoalveolar lavage fluid(BALF) and blood samples was significantly higher in two ventilation groups than the control group(P lt;0.05),which were higer in the high tidal volume group than in the low tidal volume group(P lt;0.05).Conclusions These results indicate that mechanical ventilation can cause lung injury,which is more serious in high tidal volume ventilation.And mechanical ventilation-induced lung injury can cause proinflammatory cytokines release,with different levels in bronchoalveolar lavage fluid and blood samples.
Citation:
DONG Chunshan,XU Zhixin,XU Ruihao,CHEN Xuehong,CHEN Rong,JIANG Tao,MIAO Yuqing,WEN Hao. The effects of mechanical ventilation-induced lung injury on concentration of proinflammatory cytokines in rats. Chinese Journal of Respiratory and Critical Care Medicine, 2008, 08(2): 135-139. doi:
Copy
Copyright © the editorial department of Chinese Journal of Respiratory and Critical Care Medicine of West China Medical Publisher. All rights reserved
1. |
Webb HH,Tierney DF.Experimental pulmonary edema due to intermittent positive pressure ventilation with high inflation pressures.Protection by positive end-expiratory pressure.Am Rev Respir Dis,1974,110:556-565.
|
2. |
Wilson MR,Choudhury S,Goddard ME,et al.High tidal volume upregulates intrapulmonary cytokines in an in vivo mouse model of ventilator-induced lung injury.J Appl Physiol,2003,95:1385-1393.
|
3. |
Kotani N,Takahashi S,Sessler DI,et al.Volatile anesthetics augment expression of proinflammatory cytokines in rat alveolar macrophages during mechanical ventilation.Anesthesiology,1999,91:187-197.
|
4. |
Nishina K,Mikawa K,Takao Y,et al.ONO-5046,an elastase inhibitor,attenuates endotoxin-induced acute lung injury in rabbits.Anesth Analg,1997,84:1097-1103.
|
5. |
Arold SP,Mora R,Lutchen KR,et al.Variable tidal volume ventilation improves lung mechanics and gas exchange in a rodent model of acute lung injury.Am J Respir Crit Care Med,2002,165:366-371.
|
6. |
Kolobow T,Moretti MP,Fumagalli R,et al.Severe impairment in lung function induced by high peak airway pressure during mechanical ventilation.An experimental study.Am Rev Respir Dis,1987,135:312-315.
|
7. |
Walder B,Fontao E,Totsch M,et al.Time and tidal volume-dependent ventilator-induced lung injury in healthy rats.Eur J Anaesthesiol,2005,22:785-794.
|
8. |
Matthay MA,Folkesson HG,Clerici C.Lung epithelial fluid transport and the resolution of pulmonary edema.Physiol Rev,2002,82:569-600.
|
9. |
Horinouchi H,Wang CC,Shepherd KE,et al.TNF alpha gene and protein expression in alveolar macrophages in acute and chronic hyperoxia-induced lung injury.Am J Respir Cell Mol Biol,1996,14:548-555.
|
10. |
Gullo A,Berlot G,Viviani M.The role of adult respiratory distress syndrome in the multiple organ dysfunction syndrome.Acta Anaesthesiol Scand Suppl,1996,109:70-73.
|
11. |
Takata M,Abe J,Tanaka H,et al.Intraalveolar expression of tumor necrosis factor-alpha gene during conventional and high-frequency ventilation.Am J Respir Crit Care Med,1997,156:272-279.
|
12. |
Stüber F,Wetegrove S,Schröder S,et al.Release of cytokines by low-PEEP high tidal volume ventilation in patients with ALI.Am J Respir Crit Care Med,1999,159:A457.
|
- 1. Webb HH,Tierney DF.Experimental pulmonary edema due to intermittent positive pressure ventilation with high inflation pressures.Protection by positive end-expiratory pressure.Am Rev Respir Dis,1974,110:556-565.
- 2. Wilson MR,Choudhury S,Goddard ME,et al.High tidal volume upregulates intrapulmonary cytokines in an in vivo mouse model of ventilator-induced lung injury.J Appl Physiol,2003,95:1385-1393.
- 3. Kotani N,Takahashi S,Sessler DI,et al.Volatile anesthetics augment expression of proinflammatory cytokines in rat alveolar macrophages during mechanical ventilation.Anesthesiology,1999,91:187-197.
- 4. Nishina K,Mikawa K,Takao Y,et al.ONO-5046,an elastase inhibitor,attenuates endotoxin-induced acute lung injury in rabbits.Anesth Analg,1997,84:1097-1103.
- 5. Arold SP,Mora R,Lutchen KR,et al.Variable tidal volume ventilation improves lung mechanics and gas exchange in a rodent model of acute lung injury.Am J Respir Crit Care Med,2002,165:366-371.
- 6. Kolobow T,Moretti MP,Fumagalli R,et al.Severe impairment in lung function induced by high peak airway pressure during mechanical ventilation.An experimental study.Am Rev Respir Dis,1987,135:312-315.
- 7. Walder B,Fontao E,Totsch M,et al.Time and tidal volume-dependent ventilator-induced lung injury in healthy rats.Eur J Anaesthesiol,2005,22:785-794.
- 8. Matthay MA,Folkesson HG,Clerici C.Lung epithelial fluid transport and the resolution of pulmonary edema.Physiol Rev,2002,82:569-600.
- 9. Horinouchi H,Wang CC,Shepherd KE,et al.TNF alpha gene and protein expression in alveolar macrophages in acute and chronic hyperoxia-induced lung injury.Am J Respir Cell Mol Biol,1996,14:548-555.
- 10. Gullo A,Berlot G,Viviani M.The role of adult respiratory distress syndrome in the multiple organ dysfunction syndrome.Acta Anaesthesiol Scand Suppl,1996,109:70-73.
- 11. Takata M,Abe J,Tanaka H,et al.Intraalveolar expression of tumor necrosis factor-alpha gene during conventional and high-frequency ventilation.Am J Respir Crit Care Med,1997,156:272-279.
- 12. Stüber F,Wetegrove S,Schröder S,et al.Release of cytokines by low-PEEP high tidal volume ventilation in patients with ALI.Am J Respir Crit Care Med,1999,159:A457.