Efect of Pseudomonas aeruginosa quorum-sensing systems on airway mucus hypersecretion_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

ZHANG Ping , YANG Qimei ,  SHEN Ce

Department of Respiratory Medicine，The Sixth Hospital Affiliated to Shanghai Jiaotong University．Shanghai，200233，ChinaCorresponding author：SHEN Ce，E-mail：lyshence@gmail.com;

Corresponding author：

SHEN Ce, Email: lyshence@gmail.com

Keywords：

Pseudomonas aeruginosa; Quorum-sensing systems; Airway mucus hypersecreti

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Objective To examine the effects of Pseudomonas aeruginosa(PA)quorum-sensing systems on airway mucus hypersecretion．Methods Sixty Sprague-Dawley rats were intubated with a silicone tube pre-coated with PAO1(wild-type PA strain)，PAO1-JP2(quorum-sensing-mutant strain)or saline in the bronchus．After 28 days，the mRNA and protein expression of MUC5AC in the rats’bronchial epithelia were detected by RT-PCR，alcian blue／periodic acid—Schif(AB／PAS)staining and enzyme linked immunosorbent assay(ELISA)．Results In the PAO1 group，bronchiolar epithelium goblet cells by AB／PAS staining was significantly more than those in the PAO1-JP2 and control groups(both P lt;0．05)．The expression level of MUC5AC mRNA in the PAO1 group was significantly higher than those in the PAO1-JP2 and control groups(both P lt;0．05)．The ELISA showed that the concentration of MUC5AC protein in bronchoalveolar lavage fluid(BALF)in the PAO1 group was much higher than that in the PAO1-JP2 group(P lt;0．05)．Conclusion PA quorum-sensing system plays an important role in airway mucus hypersecretion

Citation： ZHANG Ping,YANG Qimei,SHEN Ce. Efect of Pseudomonas aeruginosa quorum-sensing systems on airway mucus hypersecretion. Chinese Journal of Respiratory and Critical Care Medicine, 2008, 08(2): 93-96. doi: Copy

1.	de Kievit TR，Iglewski BH．Bacterial quorum sensing in pathogenic relationships．Infect Immun，2000，68：4839-4849．.
2.	Imamura Y，Yanagihara K，Mizuta Y，et al．Azithromycin inhibits MUC5AC production induced by the Pseudomonas aeruginosa autoinducer N一(3-Oxododecanoy1)homoserine lactone in NCI．H292 Cells．Antimicrob Agents Chemother．2004，48：3457-3461．.
3.	Mill MB，Bassler BL．Quorum sensing in bacteria．Annu Rev Microbiol，2001，55：165-199．.
4.	Schaber JA，Carry NL，McDonald NA，et al．Analysis of quorum sensing-·deficient clinical isolates of Pseudomonas aeruginosa．J Med Microbiol，2004，53：841．853．.
5.	He SH，Zheng J，Duan MK．Induction of mucin secretion from human bronchial tissue and epithelial cells by rhinovirus and lipopolysaccharide．Acta Pharmacol Sin，2004．25：1176．1181．.
6.	Li JD，Feng W ，Gallup M，et al．Activation of NF-kappaB via a Src-dependent Ras-·MAPK··pp90rsk pathway is required for Pseudomonas aeruginosa induced mucin overproduction in epithelial cells．Proc Natl Acad Sci USA，1998，95：5718-5723．.
7.	Kohri K，Ueki IF，Shim JJ，et al．Pseudomonas aeruginosa induces MUC5AC production via epidermal growth factor receptor．Eur Respir J，2002，20：1263-1270．.
8.	Nouwens AS，Beatson SA，Whitchurch CB，et al．Proteome analysis of extracellular proteins regulated by the las and rhl quorum sensing systems in Pseudomonas aeruginosa PAO1．Microbiology，2003，149：13 1 1·1322.

1. de Kievit TR，Iglewski BH．Bacterial quorum sensing in pathogenic relationships．Infect Immun，2000，68：4839-4849．.
2. Imamura Y，Yanagihara K，Mizuta Y，et al．Azithromycin inhibits MUC5AC production induced by the Pseudomonas aeruginosa autoinducer N一(3-Oxododecanoy1)homoserine lactone in NCI．H292 Cells．Antimicrob Agents Chemother．2004，48：3457-3461．.
3. Mill MB，Bassler BL．Quorum sensing in bacteria．Annu Rev Microbiol，2001，55：165-199．.
4. Schaber JA，Carry NL，McDonald NA，et al．Analysis of quorum sensing-·deficient clinical isolates of Pseudomonas aeruginosa．J Med Microbiol，2004，53：841．853．.
5. He SH，Zheng J，Duan MK．Induction of mucin secretion from human bronchial tissue and epithelial cells by rhinovirus and lipopolysaccharide．Acta Pharmacol Sin，2004．25：1176．1181．.
6. Li JD，Feng W ，Gallup M，et al．Activation of NF-kappaB via a Src-dependent Ras-·MAPK··pp90rsk pathway is required for Pseudomonas aeruginosa induced mucin overproduction in epithelial cells．Proc Natl Acad Sci USA，1998，95：5718-5723．.
7. Kohri K，Ueki IF，Shim JJ，et al．Pseudomonas aeruginosa induces MUC5AC production via epidermal growth factor receptor．Eur Respir J，2002，20：1263-1270．.
8. Nouwens AS，Beatson SA，Whitchurch CB，et al．Proteome analysis of extracellular proteins regulated by the las and rhl quorum sensing systems in Pseudomonas aeruginosa PAO1．Microbiology，2003，149：13 1 1·1322.

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Efect of Pseudomonas aeruginosa quorum-sensing systems on airway mucus hypersecretion

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