Objective  To explore changes of 3’-glutamylcysteine synthetase( γ-GCS)and reduced glutathione(GSH)in patients with bronchial asthma．Methods  Ten patients with acute asthma were enrolled and treated for six weeks according to guideline recommendations．Levels of -GCS，GSH and malondialdehyde(MDA)in total cells in induced sputum and GSH，MDA，reactive oxygen(ROS)in selum were measured and compared before and after therapy．Ten healthy volunteers were as normal contro1．Meanwhile，the pulmonary function(FEVl％pred)was measured and asthmatic symptoms were quantified using Hogg’s way．Results  A．In serum and sputum of the asthma patients，GSH were lower and MDA were higher before treatment than those of the control(P lt;0．01)．And -GCS in induced sputum
were higher before treatment than those of contro1．B．After treated for six weeks．levels of GSH in serum and sputum of the asthma patients increased copmpared to baseline(all P lt;0．01)，but were still lower than that of control(P lt;0．05)．Activities of MDA in serum and sputum and -GCS in sputum were elevated compared to baseline(P lt;0．01)，but still higher than that of control(all P lt;0．05)．C．Levels of GSH in serum of all patients were correlated negatively witll asthmatic symptom scores and levels of MDA and ROS(r=－0.701，－0.901，－0.878；P lt;0.05， lt;0.01， lt;0.01)．There was a positive relationship between levels ofGSH in serum and FEV1％pred(r=0．854，P lt;0．01)．In induced sputum，activities of 3’-GCS in all patients was correlated positively with their asthmatic symptom scores and level of MDA f r=0．804，0.926；P lt;0.05， lt;0.叭)．Conclusion  γ-GCS and GSH may participate the reaction of

Citation： ZHU Liming,CAO Shoudong,DAI Aiguo. Changes of γ-glutamyleysteine synthetase an d reduced glutathione in patients with bronchial asthma. Chinese Journal of Respiratory and Critical Care Medicine, 2008, 08(6): 459-462. doi: Copy