Objective To evaluate the effects of Shengji Yuhong collagen on promoting angiogenesis of the ischemia tissues and probe the possible mechanisms.
Methods Forty-eight Wistar rats were divided by random method of paired into blank group, control group (collagen),and experimental group (Shengjiyuhong collagen). After made the rats hind limb ischemia model, collagens with or without the extracts of Shengji Yuhong Gao were randomly paired implanted locally in hind limb ischemia tissues of rats in experimental group or control group. The samples of collagens and tissues about 0.5 cm large surrounding the collagen were explanted respectively on day 3,7, 14, and 28 for detected the hemog-lobin contents in colagen, microvascular counting by using CD34 immunohistochemical markers, and the expressions of HIF-1α mRNA and VEGF mRNA by using real-time fluorescent quantitative RT-PCR. The blood perfusion of the ischemic tissues at each time were determined by using laser speckle imaging system of Moor-FLPI.
Results The results of Moor-FLPI showed that the obvious ischemia condition after model made, the blood perfusion was significantly lower than that before operation (P<0.01). On day 3 after operation it showed obvious congestion in the ischemic tissues, and from day 7 to day 14, it showed the ischemia state locally till day 28 after operation which showed improved situation of ischemic. Except for the day 3, the blood perfusion of experimental group were higher than those of blank group (P<0.05). There was no statistical significance between the blank group and control group (P>0.05). The blood perfusion on day 7 and day 14 after operation of experimental group were higher than those of control group (P<0.05). The hemoglobin contents
of each time point in the experimental group were higher than those in the control group (P<0.01). The microvascular counting on day 7 and day 14 in experimental group were higher than those of control group (P<0.05). The expressions of HIF-1α mRNA and VEGF mRNA at each time point of experimental group were higher than those of control group and blank group (P<0.05), and there was no significant differences between the control group and blank group (P>0.05).
Conclusion The effects on promoting angiogenesis of rat hind limb ischemia tissues with Shengji Yuhong collagen may though inducing the expressions of HIF-1 α mRNA and VEGF mRNA locally.
Citation:
YAO Chang,CHEN Dexuan,BIAN Weihe,ZHANG Xiaoqing,YIN Heng,GUO Mengmeng,CAO Dongyang.. Experimental Research on Promoting Angiogenesis on Rat Hind Limb Ischemia Tissues with Local Implantation of Shengji Yuhong Collagen. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2013, 20(4): 389-394. doi:
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- 1. 李曰庆主编. 中医外科学[M]. 北京: 人民卫生出版社, 2006:.
- 2. Armstrong D, Bortz P. An integrative review of pressure relief in surgical patients[J]. AORN J, 2001, 73(3):645-648, 650-653, 656-657.
- 3. 杨华, 刘海平, 汪虹, 等. 他汀类药物促进血管新生及在创面修复们中的意义[J]. 中华损伤与修复杂志(电子版), 2010, 5(6): 803-806.
- 4. 王君, 汪虹, 刘海平, 等. 粒细胞集落刺激因子促进血管新生及损伤修复的研究进展[J]. 中华损伤与修复杂志(电子版), 2009, 4(2):207-211.
- 5. 缪雪华, 张宇轩, 陈运, 等. 生肌玉红胶原海绵促进胶原合成与组织愈合的研究[J]. 南京中医药大学学报, 2012, 28(4): 337-341.
- 6. 严婷婷, 熊猛, 周勇, 等. 生肌玉红胶原海绵修复创面损伤[J]. 中国组织工程研究与临床康复, 2011, 15(29):5367-5370.
- 7. 李永刚, 高卫卫, 姚昶. 生肌玉红明胶海绵对机械性大鼠创面微循环影响的实验研究[J]. 江苏中医药, 2010, 42(12):71-73.
- 8. 高卫卫, 姚昶. 生肌玉红明胶海绵治疗慢性下肢溃疡32例临床观察[J]. 江苏中医药, 2010, 42(3): 46-47.
- 9. 姚昶, 孙海舰, 姚涌晖, 等. 生肌玉红明胶海绵促进机械性创面肉芽生长的实验研究[J]. 医学研究杂志, 2009, 38(5):62-65, 139.
- 10. Yao C, Markowicz M, Pallua N, et al. The effect of cross-linking of collagen matrices on their angiogenic capability[J]. Biomaterials, 2008, 29(1):66-74.
- 11. Markowicz M, Koellensperger E, Steffens GC, et al. The impactof vacuum freeze-drying on collagen sponges after gas plasma steri-lization[J]. J Biomater Sci Polym Ed, 2006, 17(1-2): 61-75.
- 12. 杨盛家, 陈兵, 罗涛. 大鼠后肢急性缺血模型的构建及评估[J]. 中国普通外科杂志, 2009, 18(6): 580-584.
- 13. Greco A, Ragucci M, Liuzzi R, et al. Repeatability, reproducibility and standardisation of a laser Doppler imaging technique for the evaluation of normal mouse hindlimb perfusion[J]. Sensors (Basel), 2012, 13(1):500-515.
- 14. Kushibiki T, Saito A, Hayashi S, et al. Quantitative estimates of vascularity in a collagen-based cell scaffold containing basicfibroblast growth factor by noninvasive near-infrared spectroscopyfor regenerative medicine[J]. Photomed Laser Surg, 2008, 26(3):247-250.
- 15. Gayed BA, O’Malley KJ, Pilch J, et al. Digoxin inhibits blood vessel density and HIF-1α expression in castration-resistant C4-2 xenograft prostate tumors[J]. Clin Transl Sci, 2012, 5(1):39-42.
- 16. Greenberger S, Adini I, Boscolo E, et al. Targeting NF-κB in infantile hemangioma-derived stem cells reduces VEGF-A expression[J]. Angiogenesis, 2010, 13(4):327-335.
- 17. 王云飞, 阙华发, 徐杰男, 等. “祛腐化瘀补虚生肌外治法治疗慢性下肢溃疡的临床示范性研究”的研究方案[J]. 中西医结合学报, 2012, 10(2): 166-175.
- 18. 宋晓晶, 张栋, 马慧敏, 等. 电针对小鼠肝脏血流灌注量影响的激光散斑成像显示[J]. 激光生物学报, 2011, 20(1):15-21.
- 19. 杨盛家, 陈兵, 佟铸, 等. 应用激光多普勒血流仪对大鼠后肢缺血模型血流动力学的观察[J]. 中国普外基础与临床杂志,2011, 18(2):137-142.
- 20. 郑见宝, 孙学军, 王炜, 等. HIF-1α与CDX2在结直肠腺癌组织中的表达及意义[J].中国普外基础与临床杂志, 2010, 17(12):1265-1269.
- 21. 周斌, 张培建, 李勇, 等. VEGF参与低氧预适应对移植肝肝内胆管上皮细胞的保护作用[J]. 中国普外基础与临床杂志, 2009, 16(5):365-369.
- 22. 李明琰, 何文凯, 陈建威, 等. Ad-HIF-1α-Trip肌肉注射对大鼠缺血肢体血管新生和血液灌注的影响[J]. 第三军医大学学报, 2012, 34(15):1518-1522.
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