Study on miRNA-224 in Colorectal Cancer with Hepatic Metastasis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

JI Xiaoxin , YANG Yun , YU Deming ,  LUO Chengyu

Department of General Surgery， Fuxing Hospital of Capital Medical University， Beijing 100038， China;

Corresponding author：

LUO Chengyu, Email: luochengyu@163.com

Keywords：

Colorectal neoplasm; microRNA-224; Hepatic metastasis; miRNA microarray

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective 　To explore the microRNA （miRNA） expression changes and related miRNA characteristics of colorectal cancer （CRC） with hepatic metastasis by miRNA microarray.
Methods 　The fresh specimens of primary CRC were collected in 10 patients during operation， which with hepatic metastasis or not. miRNA microarray analysis was performed to compare the miRNA expression levels in two groups. The different expression levels of miRNA were validated by quantitative real-time PCR analysis.
Results 　A total of six dysregulated miRNAs were identified in the CRC patients with hepatic metastasis comparing with CRC patients without hepatic metastasis， including 3 up-regulated miRNAs （miR-224， miR-1236， and miR-622） and 3 down-regulated miRNAs （miR-155， miR-342-5p， and miR-363）， and the quantitative real-time PCR result of miR-224 consisted with the microarray finding.
Conclusions 　miR-224 may be involved in the process of CRC with hepatic metastasis pathogenesis. miR-224 would be a research direction on a new biomarker or therapic method in CRC with hepatic metastasis.

Citation： JI Xiaoxin,YANG Yun,YU Deming,LUO Chengyu. Study on miRNA-224 in Colorectal Cancer with Hepatic Metastasis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2013, 20(5): 499-502. doi: Copy

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28.	， 449(7163)：682-688.

1. Manfredi S， Lepage C， Hatem C， et al. Epidemiology and management of liver metastases from colorectal cancer[J]. Ann Surg， 2006， 244(2)：254-259.
2. Bolstad BM， Irizarry RA， Astrand M， et al. A comparison of normalization methods for high density oligonucleotide array data based on variance and bias[J]. Bioinformatics， 2003， 19(2)：185-193.
3. Livak KJ， Schmittgen TD. Analysis of relative gene expre-ssion data using real-time quantitative PCR and the 2-ΔΔCT method[J]. Methods， 2001， 25(4)：402-408.
4. Cho WC. OncomiRs：the discovery and progress of microRNAs in cancers[J]. Mol Cancer， 2007， 6：60.
5. Gregory PA， Bert AG， Paterson EL， et al. The miR-200 familyand miR-205 regulate epithelial to mesenchymal transition by targe-ting ZEB1 and SIP1[J]. Nat Cell Biol， 2008， 10(5)：593-601.
6. Zhang YJ， He XJ， Liu YL， et al. microRNA-320a inhibits tumor invasion by targeting neuropilin 1 and is associated with liver metas-tasis in colorectal cancer[J]. Oncol Rep， 2012， 27(3)：685-694.
7. Yan LX， Huang XX， Shao Q， et al. MicroRNA miR-21 overexpression in human breast cancer is associated with advanced clinicalstage， lymph node metastasis and patient poor prognosis[J]. RNA， 2008， 14(11)：2348-2360.
8. Asangani IA， Rasheed SA， Nikolova DA， et al. MicroRNA-21 (miR-21) post-transcriptionally downregulates tumor suppressor Pdcd4 and stimulates invasion， intravasation and metastasis in colorectal cancer[J]. Oncogene， 2008， 27(15)：2128-2136.
9. Wellner U， Schubert J， Burk UC， et al. The EMT-activator ZEB1 promotes tumorigenicity by repressing stemness-inhibiting microRNAs[J]. Nat Cell Biol， 2009， 11(12)：1487-1495.
10. 张璐. 在结肠腺癌中MicroRNA的表达方式与其预后和治疗效果的相关性[J]. 中国普外基础与临床杂志， 2008， 15(9)：698.
11. 张明，刘卫辉，尤楠，等. 7种microRNAs在原发性肝癌组织和癌旁组织间的差异表达及与血清肿瘤标志物水平的相关性研究[J]. 中国普外基础与临床杂志， 2010， 17(6)：562-566.
12. 颜登国，王国栋，程海玉. 基因芯片技术分析结直肠癌肝转移患者免疫基因表达的变化[J]. 中国普外基础与临床杂志， 2012， 19(11)：1182-1186.
13. Feng B， Dong TT， Wang LL， et al. Colorectal cancer migration and invasion initiated by microRNA-106a[J]. PLoS One， 2012， 7(8)：e43452.
14. Hogan NM， Joyce MR， Kerin MJ. MicroRNA expression in colorectal cancer[J]. Cancer Biomark， 2012， 11(6)：239-243.
15. Tokarz P， Blasiak J. The role of microRNA in metastatic colorectal cancer and its significance in cancer prognosis and treatment[J]. Acta Biochim Pol， 2012， 59(4)：467-474.
16. Dong Y， Wu WK， Wu CW， et al. MicroRNA dysregulation in colorectal cancer：a clinical perspective[J]. Br J Cancer， 2011， 104(6)：893-898.
17. Ma L， Teruya-Feldstein J， Weinberg RA. Tumour invasion and metastasis initiated by microRNA-10b in breast cancer[J]. Nature，.
18. Nikiforova MN， Tseng GC， Steward D， et al. MicroRNA expre-ssion profiling of thyroid tumors：biological significance and diagnostic utility[J]. J Clin Endocrinol Metab， 2008， 93(5)：1600-1608.
19. Wang Yu， Lee AT， Ma JZ， et al. Profiling microRNA expression in hepatocellular carcinoma reveals microRNA-224 up-regul-ation and apoptosis inhibitor-5 as a microRNA-224-specific target[J]. J Biol Chem， 2008， 283(19)：13205-13215.
20. Prueitt RL， YI M， Hudson RS， et al. Expression of microRNAs and protein-coding genes associated with perineural invasion in prostate cancer[J]. Prostate， 2008， 68(11)：1152-1164.
21. Mees ST， Mardin WA， Sielker S， et al. Involvement of CD40 targeting miR-224 and miR-486 on the progression of pancreatic ductal adenocarcinomas[J]. Ann Surg Oncol， 2009， 16(8)：2339-2350.
22. Hildebrand J， Rütze M， Walz N， et al. A comprehensive analysis of microRNA expression during human keratinocyte differentiationin vitro and in vivo[J]. J Invest Dermatol， 2011， 131(1)：20-29.
23. Bandrés E， Cubedo E， Agirre X， et al. Identification by real-timePCR of 13 mature microRNAs differentially expressed in colorectalcancer and non-tumoral tissues[J]. Mol Cancer， 2006， 5：29.
24. Schetter AJ， Leung SY， Sohn JJ， et al. MicroRNA expressionprofiles associated with prognosis and therapeutic outcome in colonadenocarcinoma[J]. JAMA， 2008， 299(4)：425-436.
25. Chiang Y， Song YX， Wang ZN， et al. microRNA-192， -194 and -215 are frequently downregulated in colorectal cancer[J]. Exp Ther Med， 2012， 3(3)：560-566.
26. Fu JH， Tang WT， Du P， et al. Identifying microRNA-mRNA regulatory network in colorectal cancer by a combination of expre-ssion profile and bioinformatics analysis[J]. BMC Systems Bio-logy， 2012， 6：68.
27. Mazeh H， Mizrahi I， Ilyayev N， et al. The diagnostic and prognostic role of microRNA in colorectal cancer―a comprehensive review[J]. J Cancer， 2013， 4(3)：281-295.
28. ， 449(7163)：682-688.

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Study on miRNA-224 in Colorectal Cancer with Hepatic Metastasis

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