west china medical publishers
Keyword
  • Title
  • Author
  • Keyword
  • Abstract
Advance search
Advance search

Search

find Keyword " MicroRNA-129" 1 results
  • MicroRNA-129 Promotes Cardiomyogenesis in Bone Marrow Mesenchymal Stem Cells

    Objective To explore the induction of cardiomyogenesis of microRNA-129 (mir-129) in rat bone marrowmesenchymal stem cells (BM-MSCs) and its mechanism. Methods BM-MSCs were isolated from Sprague-Dawley rats and cultured in vitro. Overexpression of mir-129 or both mir-129 and glycogen synthase kinase-3β (GSK-3β) in BM-MSCs was produced with a lentiviral vector system. All the BM-MSCs were divided into four groups: control group (MSCs),Lentiviral vectors+MSCs group (Lv-MSCs),mir-129 transfection group (mir-129-MSCs),and mir-129+GSK-3βdouble transfection group (mir-129+GSK-3β-MSCs). Five-Azacytidine (5-Aza) (10 μmol/L) was used to induce BM-MSCsdifferentiation into cardiomyocytes. On the 1st,5 th,10 th,15 th and 20 th day after induction,realtime-PCR was performedto detect mRNA levels of GATA-4,Nkx2.5 and MEF-2C. On the 10 th,15 th and 20 th day after induction,Western blottingwas performed to examine expression levels of cTnI,Desmin,GSK-3β,phosphorylated β-catenin and dephosphorylated β-catenin. Results Compared with the control group,at respective time points,mRNA levels of cardiomyogenic genes and expression levels of cardiomyocyte-related proteins of mir-129 transfection group were significantly elevated,theexpression level of GSK-3β was significantly decreased,and the ratio of dephosphorylated/phosphorylated β-catenin was significantly elevated. When both mir-129 and GSK-3β were overexpressed in BM-MSCs,mRNA levels of cardiomyogenicgenes and expression levels of cardiomyocyte-related proteins were significantly lower than those of mir-129 transfection group,and the ratio of dephosphorylated/phosphorylated β-catenin was significantly decreased. Conclusion Overexpression of mir-129 can promote cardiomyogenesis of rat BM-MSCs possibly via inhibiting GSK-3β production and thus decreasing the inhibition of phosphorylation of β-catenin which then enters the nucleus and activates downstream signaling pathways that regulate cardiomyogenic differentiation of BM-MSCs.

    Release date:2016-08-30 05:47 Export PDF Favorites Scan
1 pages Previous 1 Next

Format

Content