【摘要】目的探讨乳腺癌根治术式改良的方法及其在减少术后早期并发症中的意义。方法398例乳腺癌患者，行乳腺癌根治术22例，Patey氏手术83例，Anchincloss手术247例，改良Anchincloss手术46例。比较不同术式术后早期并发症发生情况。结果乳腺癌根治术术后的早期并发症发生率明显高于其它术式（Plt;0.01）； Patey氏手术与Anchincloss手术相比，皮下积液、皮瓣坏死的发生率差异无显著性意义（Pgt;0.05），但患侧上肢水肿的发生率前者高于后者（Plt;0.01）； 改良Anchincloss手术的并发症发生率明显低于前3种术式（Plt;0.01），且其手术时间比其它术式平均缩短20 min。结论改良Anchincloss手术能明显减少术后早期并发症发生率并缩短手术时间。
Objective To observe the effects of hydrogen peroxide on the expression of transforming growth factorβ1(TGF-β1) and Smad3 protein in A549 cells. Methods A549 cells were cultured with different concentrations of hydrogen peroxide. MTT assay was used to determine the cell growth and survival rates. The level of TGF-β1 and p-Smad3 protein were detected by western blotting. Results It was observed that hydrogen peroxide significantly inhibit proliferation of A549 cells. When the concentration of hydrogen peroxide was 1.0 mmol/L, the inhibition ratio reaches 46.34%, and the level of TGF-β1 and p-Smad3 protein were increased in a time-dependence manner and reached a peak after 24 h, then decreased a little but also remained at high level. Conclusions In the early oxidative damage, A549 cells express high level of TGF-β1 and p-Smad3 protein. It may be relevant to tissue repair and remodeling after lung injury.