ObjectiveTo study the feasibility of the establishment of transplantated hepatic cancer models in SD rats. MethodsThe male weaning SD rats were inoculated and transgenerated from the celiar transplantation tumor rats (W2562k) by intraperitoneal injection of abdominal fluid of tumor cells, another weaningSD rats were prepared for the models of solid tumor by subcutaneous inoculation of the abdominal fluid of tumor cells. The solid tumors were cut to pieces of 0.2 cm×0.2 cm×0.3 cm,which were implanted into the liver of the adult male SD rats, and the transplantation hepative tumor models were established. After 7 days, the volume and weight of tumor mass was measured. ResultsThe successful rate of model was 100% (82/82), the natural extinctive rate was 0 (0/82), the successful rate of inoculation was 100% (15/15). ConclusionSD rats are economical, the successful rate of model was high, the natural extinctive low, so the SD rats are ideal selection in establishing transplantation liver cancer model.
目的探讨近端脾肾分流联合贲门周围血管离断术治疗肝硬变门脉高压症的疗效。方法回顾分析我院1994~2004年10年期间应用近端脾肾分流联合贲门周围血管离断术治疗62例肝硬变门脉高压病例。结果本组无手术死亡，随访56例，随访时间4~56个月，随访结果： 26例肝功能较前有所改善，49例静脉曲张明显减轻，无静脉破裂出血； 发生吻合口血栓5例，肝性脑病3例，因肝衰死亡3例。结论近端脾肾分流联合贲门周围血管离断术治疗肝硬变门脉高压症效果好，疗效满意。
目的探讨肝癌切除后行区域性化疗治疗肝癌的效果。方法原发性肝癌18例，转移性肝癌3例，均行肿瘤切除，病侧肝动脉结扎，胃网膜右静脉置入药泵，于术中经药泵注入5Fu，拆线后用5Fu（总量6 g）、丝裂霉素（总量8 mg）及阿霉素（总量60 mg）经药泵注入化疗。术后前3年，每年化疗3个疗程，第4～5年，每年化疗1～2个疗程。结果21例中随访3年以上12例，6例失访，3例死亡，本组资料3年生存率为57%。结论肝癌切除后，结扎病侧肝动脉，行门静脉区域性化疗，疗效显著。
目的 总结闭孔疝的临床诊断、治疗及手术经验。方法 回顾性分析笔者所在医院2010年7月至2012年7月期间收治的5例闭孔疝患者的临床资料。结果 5例患者查体时4例具有明确的Howship-Romberg征，均未发现Hannington-Kiff征。4例术前行腹部立卧位X线平片提示肠梗阻，1例提示不全性肠梗阻。5例均行腹部及盆腔CT平扫，仅1例怀疑病变侧可疑疝囊影像。术前均未确诊，均行剖腹探查，术中证实患者均为单侧闭孔疝嵌顿，嵌顿物为小肠，且所嵌顿小肠均已坏死，遂行坏死肠段切除、闭孔内口处缝扎疝囊颈。术后并发肺部感染3例，肠瘘1例；痊愈4例，死亡1例，死亡原因为肺部感染及肠瘘。5例患者的平均住院时间为10.5d（5～14d）。存活的4例患者均随访12个月，无复发。结论 闭孔疝的临床表现不典型，对于年老、体弱的患者，出现不明原因肠梗阻，且伴有明确的Howship-Romberg征时应该高度怀疑该病的可能。早期诊断和早期手术是提高闭孔疝患者生存率的有效手段。
ObjectiveTo discuss the treatment methods and outcome of hilar cholangiocarcinoma. MethodsFrom January 2002 to December 2008, 81 cases of hilar cholangiocarcinoma were retrospectively analyzed. ResultsAmong 81 patients, there were 55 males and 26 females, ages were from 38 to 72 years with an average age 57.5 years. In BismuthCorlette classification, 5 cases were type Ⅰ, 15 cases type Ⅱ, 14 cases type Ⅲa, 14 cases type Ⅲb, 33 cases type Ⅳ, according to the preoperative results of MRCP, but the classification of 15 cases were not consistent to the preoperative results (5 cases type Ⅱ, 8 cases type Ⅲ, 2 cases type Ⅳ) according to the results of intraoperative exploration. The rates of complications of radical operation, palliative operation, internal biliary drainage, and external biliary drainage were 54.5%(12/22), 58.8%(10/17), 23.8%(5/21), and 66.7%(14/21), respectively. The rate of complications of internal biliary drainage was lower than that of the other three methods (Plt;0.01), there were no significant differences among the other three methods. The 1, 2, 3, and 5year survival rates of 22 patients with radical operation, 17 patients with palliative operation, 21 patients with internal biliary drainage, 21 patients with external biliary drainage were 75.0%, 60.0%, 38.3%, 2.6%; 72.7%, 26.5%, 4.2%, 0; 50.5%, 15.8%, 2.2%, 0; 30.6%, 8.5%, 0, 0, respectively. The median survival time was 29.5 months, 13.8 months, 10.5 months, and 8.3 months, respectively. Survival rate of radical operation was higher than that of palliative operation (χ2=14.20, P=0.000 3), palliative operation was higher than that of internal biliary drainage (χ2=4.50, P=0.040 5), and internal biliary drainage was higher than that of external biliary drainage (χ2=4.45, P=0.040 1). ConclusionsThe BismuthCorlette classification is a guide to the required surgery, but the results of intraoperative exploration decides the final classification and operative method. Radical resection is the main related factors influencing the therapy efficacy of hilar cholangiocarcinona. Basinstyle anastomosis and T type supportingtube is the first choice of palliative operation. External drainage, to the full, is avoided.
ObjectiveTo summarize the effect of the multi-disciplinary treatment (MDT) for a patient with giant pancreatic cystic lymphangioma.MethodsThe clinical data of a patient with pancreatic lymphangioma admitted to the Affiliated Hospital of North Sichuan Medical College in March 2020 was retrospectively analyzed, then reviewed the literatures, and summarized the preoperative diagnosis, treatment, and management of perioperative period and long-term period after surgery of this rare disease.ResultsAfter admission, the patient was diagnosed as pancreatic tumor by various imaging and laboratory examinations. The nature was unknown. After discussion by MDT, it was decided to undergo a total pancreatectomy (TP). After the operation, drugs and diet were given to regulate blood glucose. The patient’s tumor disappeared after the operation, and no tumor recurrence was found in the three months after discharge.ConclusionsPancreatic lymphangioma is rare and lacks a clear diagnosis and treatment plan. The MDT mode can bring a clearer diagnosis and more effective treatment for it.
ObjectiveTo understand the research progress of the matrix metalloproteinases (MMPs) family in regulating the development of hepatocellular carcinoma (HCC) and its mechanism, in order to provide a reference for the basic research and clinical diagnosis and treatment of HCC. MethodThe relevant literature on the regulation of HCC occurrence, development, and mechanisms by MMPs both domestically and internationally in recent years was reviewed. ResultsThe extracellular matrix (ECM) microenvironment of HCC cells determined the invasiveness and degree of metastasis of tumor cells. The degradation and remodeling of ECM during epithelial mesenchymal transition (EMT) were the main factors contributing to the invasion and metastasis of HCC. The abnormal expression of most members of the MMPs family could lead to ECM breakdown, cell invasion and attachment, and markedly accelerate the process of EMT, thereby promoting the invasion and metastasis of HCC cells. At present, there were many MMPs related to the development of HCC, including MMP-1, 2, 3, 7, 9, 12, 13, 14. The relevant research on the relation between MMP-8, 10, 11, 15, 16, 20, 21, 26 or 28 and the development of HCC was relatively limited, while the exact research on the relationship between the MMP-17, 19, 23, 24, 25 or 27 and HCC development had not been retrievaled. ConclusionsThe MMPs family members (especially MMP-2, 3, 7, 9, 10, 12) play a crucial role in the progression of HCC, including proliferation, invasion, and metastasis. Further exploration of the potential intrinsic relation between all members of the MMPs family members and the development of HCC is crucial for predicting HCC metastasis potentiality and prognosis, as well as developing new or improved targeted anti-cancer therapies for HCC.