OBJECTIVE: To further explore the effects of fibroblast growth factor on soft tissue repair. METHODS: Based on the data from our experiments and clinical trial and data from other reports, a further reconsideration about fibroblast growth factor and soft tissue repair was demonstrated, including embryonic development, histology, animal experiments, clinical trial and prospect. RESULTS: Amounts of basic and clinical data showed that fibroblast growth factor was needed in embryonic development. Exogenous fibroblast growth factor could accelerate wound healing. CONCLUSION: Fibroblast growth factor is a bioactive protein which can obviously promote wound healing, it has broad prospects of clinical application.
Objective To introduce the basic research and cl inical potential of the hair foll icle stem cells related signal transduction in prol iferation and differentiation. Methods The recent original articles about the hair foll icle stem cells were extensively reviewed. Results Many different signal pathways had been involved in the skin development and self-newals.The hair foll icle stem cells could play an important role in the skin self-renewal and regeneration which were modulated by several different signal pathways, which included bone morphogenetic protein/transforming growth factor β, Wnt, Notch and ectodysplasin A genes. Conclusion The hair foll icle stem cells may be a future approach to repair cutaneous wounds as a cell therapy．
Objective To review the advance in the experimental studies and evaluate the potential therapeutic application of the mesenchymal stem cells(MSCs). Methods The related articles published in China and theother countries during the recent years were extensively reviewed and analyzed. Results The MSCs were widely used in the cell-transplantation therapy and the tissue engineering because of their pluripotency of differentiation into various kinds of cells. They were also frequently used in the gene therapy because they could stably express the transfected objective genes. Because of their immunomodulatory function, the MSCs could also be used in the immunotherapy. Conclusion The MSCs are the stem cells, which have characteristics of renewing themselves, having multipotency, and being easy to undergo amplification in vitro.The MSCs are ideal target cells for the cell therapy, tissue engineering, gene therapy, and immunotherapy.
Objective To investigate the effects of the nerve, endocrine, and immune factors on the process of wound healing and regeneration of the skin after injury and to review the research work in the past years in this area. Methods The prospective study was made to explore the relationship among the nerve, endocrine, immune factors, and skin tissue repair and regeneration, and to summarize the recent advance in this area. Results The nerve, endocrine, and immune factors played an important role in the repair and generation of the skin after injury. Conclusion As there has been fewer studies made in this field, we should reinforce the related basic research in this field.
Objective To introduce the related issues in the clinical translational application of adipose-derived stem cells (ASCs). Methods The latest papers were extensively reviewed, concerning the issues of ASCs production, management, transportation, use, and safety during clinical application. Results ASCs, as a new member of adult stem cells family, bring to wide application prospect in the field of regenerative medicine. Over 40 clinical trials using ASCs conducted in 15 countries have been registered on the website (http://www.clinicaltrials.gov) of the National Institutes of Health (NIH), suggesting that ASCs represents a promising approach to future cell-based therapies. In the clinical translational application, the related issues included the quality control standard that management and production should follow, the prevention measures of pathogenic microorganism pollution, the requirements of enzymes and related reagent in separation process, possible effect of donor site, age, and sex in sampling, low temperature storage, product transportation, and safety. Conclusion ASCs have the advantage of clinical translational application, much attention should be paid to these issues in clinical application to accelerate the clinical translation process.
Objective To investigated the distribution of epidermal stem cells in rat fullthickness wound tissues during the wound healing process and toelucidate the roles of epidermal stem cells in wound repair in vivo.Methods Eighty circular fullthickness wounds were produced on both sides of the back in 20 male Wistar rats labeled with BrdU 60 days previously (4 wounds in each rat). BrdU, β1 integrin and keratin 19 (K19) were employed to determine the epidermal stem cells with SP immunohistochemical methods, and the epithelialization wasdetermined with routine histological methods of HE staining on the 3rd, 7th, 14th, and 21st days after operation.Results No cells with positive immunostaining for β1 integrin, K19 and BrdU were found in granulation tissue of woundin both groups during the healing process. However, a few scattered β1 integrin and K19 positive cells were found within the stratum spinosum and stratum granulosum of the epidermis on the wound edges on the 3rd day post-injury. And these positive cells gradually became more and more in number, and mostly concentrated on the border of wound edges till the wounds healed. In addition, the number of positive cells for β1 integrin and K19 in the infected wounds was less than that in non-infected wounds. These positive cells for β1 integrin and K19 staining on the wound edge were also positively stained with BrdU in the cellular nuclei. Conclusion The above results indicate that ectopia of epidermal stem cells present a major function during wound epithelialization.
Objective To investigate the expression of ectodysplasin (EDA) genesignaling and its relationship with the development and regeneration of sweat glands. Methods The articles concerned in the latest years wereextensively reviewed. Results EDA gene is an important signaling pathway associated with the developmental procedure of sweat glands in early fetal stage. Abnormality or depletion of function in sweat glands partially owed to the defect of EDA gene. Conclusion EDA signaling has its biological significance in inducing development and morphogenesis of sweat glands and in maintaining physiological function of skin. It could be a new approach to repair or regenerate the sweat glands for clinical therapy by regulating the expression of EDA gene.
Objective To study the relationship between the adipose tissues and cutaneous morphogenesis on wound healing. Methods Adipose tissues’ participation in regulation of food intake, energy expenditure, fuel metabolism, and a variety of other physiological processes was reviewed in the latest literature. The wound healing was regulated by endocrine, paracrine, and adipocytederived molecules. Results Several factors secreted by adipose tissues (leptin, cytokines, growth factors, lipids, metallothioneins, ect) regulated wound healing. Conclusion Adipose tissues may play a vital role in the process of wound healing. Further understanding of the complex interaction between adipose cells and cutaneous morphogenesis is essential to explore the mechanism of wound healing.