目的:应用应变率探讨不同左心室构型的原发性高血压患者左心室长轴方向应变－应变率成像的变化,及其与EF、FS及二尖瓣血流测量评价左室功能的对照研究。方法:采用M型、二维、多普勒超声及应变率成像技术,测量左室室壁厚度、左室内径、EF、FS,二尖瓣血流频谱E、A及左室平均应变ε,应变率S、e、a。结果:高血压离心性肥厚组EF、FS明显低于对照组,高血压其余各组EF、FS与对照组无统计学差异;收缩期应变率S应变ε在五组间差异均有显著性意义:高血压各组较正常对照组减小(I--Ⅴ呈递减);舒张期应变率e减低、a增高,e/a比值减小,各组间存在统计学差异(Plt;0.05); E/A,e/a结果大体一致。结论:应变率成像为临床提供了一个敏感、简便、可靠的评价原发性高血压患者左室心肌功能的指标。
ObjectiveTo investigate effects of high expression of miR-499a-5p on lung injury in rats with acute respiratory distress syndrome (ARDS) by targeting matrix metallopeptidase-16 (MMP-16).MethodsThe experiment set up sham operation group, model group, miR-499a-5p mimic group, MMP-16 group, miR-499a-5p mimic+MMP-16 group, D-ribofuranosylbenzimidazole (DRB, Nrf2 signaling pathway inhibitor) group, miR-499a-5p mimic+DRB group. A rat model of ARDS was constructed by cecal puncture. One hour before surgery, the transfection complex (50 μL) was injected into the trachea with a micro-syringe. DRB (5 mg/kg) was intraperitoneally injected 30 min before surgery. The expression levels of miR-499a-5p and MMP-16 in lung tissue were detected by RT-qPCR; Alveolar type Ⅱ epithelial cells of model group rats were separated and MMP-16 3 'UTR WT and MUT luciferase report plasmid were transfected into alveolar type Ⅱ epithelial cells with miR-499 respectively to verify the targeting relationship between miR-499 and MMP-16; the targeted relationship was verified by the dual luciferase reporter gene; lung injury was observed by hematoxylin-eosin staining; The level of inflammatory factors in bronchoalveolar lavage fluid (BALF) and the level of oxidative stress in lung tissue were detected by enzyme-linked immunosorbent assay; The expression levels of NAD(P)H: quinone oxidoreductase 1 (NQO1), heme oxygenase (HO)-1, and nuclear factor-erythroid 2-related factor 2 (Nrf2) proteins in lung tissues were analyzed by Western blotting.ResultsmiR-499a-5p was down-regulated in the lungs of ARDS model rats (P<0.01), while MMP-16 was highly expressed (P<0.01); miR-499a-5p and MMP-16 3'UTR regions had binding sites, and miR-499a-5p directly targeted negative regulation of MMP-16 expression (P<0.01); overexpression of miR-499a-5p significantly reduced the right lung wet-to-dry weight ratio in the ARDS rats (P<0.05), reduced lung tissue damage (P<0.01), and reduced tumor necrosis factor α, interleukin (IL)-1β and IL-6 levels in BALF (P<0.01), decreased malondialdehyde and myeloperoxidase levels in lung tissue, increased total anti-oxidant capacity (P<0.01), and up-regulated NQO1, HO-1, Nrf2 protein expression in lung tissue (P<0.01). However, this phenomenon was significantly reversed after the addition of MMP-16 and DRB.ConclusionOverexpression of miR-499a-5p attenuates lung injury in rats with ARDS by targeting negative regulation of MMP-16 via activating the Nrf2 signaling pathway.
Objective To investigate the ultrasonographic characteristics of acute Stanford type A aortic dissection (ATAAD) involving renal artery and its relationship with renal function. Methods The patients with ATAAD admitted to our hospital from February 2013 to May 2023 were selected. According to whether dissection involved renal artery, the patients were divided into a renal artery involved group and a renal artery uninvolved group. The general data and ultrasonic characteristics of the two groups were compared, and the relationship between ultrasonic characteristics and renal artery involvement in ATAAD patients was analyzed by logistic regression analysis and correction model. Receiver operating characteristics (ROC) curve was used to evaluate the predictive value of ultrasonic characteristics for renal artery involvement in ATAAD patients. In addition, according to blood urea nitrogen (BUN) and Serumcreatinine (Scr), patients with renal artery involvement were divided into a normal renal function group and an abnormal renal function group. The general data of the two groups were compared, and the risk effect of ultrasonic characteristics involving renal artery in renal function was analyzed by Log-binomial model. Pearson correlation analysis was used to analyze the correlation between ultrasonic features involving renal artery and renal function indexes. Results Finally 163 patients were collected, including 154 males and 89 females, aged 20 to 85 (50.06±10.46) years. There were significant differences in gender, Scr and BUN between the renal artery involved group and the non-involved group (P<0.05). Compared with the non-involved group, the ascending aorta diameter, the proportion of patients with left ventricular wall thickening and renal perfusion difference in the involved group were greater (P<0.05). Logistic regression analysis showed that after adjusting for confounder, the ultrasonic characteristics and OR values of renal artery involvement in ATAAD patients were 1.916, 2.578 and 1.969, respectively. Ascending aortal diameter, left ventricular wall thickening and poor renal perfusion were independent influencing factors of renal artery involvement (P<0.05). The ultrasonic features were effective in predicting renal artery involvement in ATAAD patients. The combined prediction of the three factors involved renal artery, and the prediction efficiency was higher. Compared with normal renal function group, BUN, Scr, ascending aorta diameter, proportion of ascending aorta widening patients and renal perfusion difference in abnormal renal function group were greater (P<0.05). Log-binomial model analysis showed that the risk ratio of increasing ascending aorta diameter, widening ascending aorta and increasing renal perfusion difference had statistical significance before and after significant adjustment (P<0.05). The results of Pearson correlation analysis showed that ascending aorta diameter, ascending aorta widening and renal perfusion difference were closely related to renal function indexes (P<0.05). ConclusionThe ultrasonic features of ATAAD involving renal artery can affect renal function and have a certain correlation, and the increase of ascending aorta diameter, widening of ascending aorta and renal perfusion difference are independent risk factors for renal function abnormalities.
ObjectiveTo study microRNA (miRNA) involved in the regulation of sinus cell differentiation by comparing sinus node, atrial myocardium, and ventricular myocardium specific miRNA expression profile differences in Kunming mice. MethodsA total of 180 Kunming mice, aged 60-90 days and weighing 35-45 g, were selected without gender differences after the method of anatomical localization for sinus node had been confirmed by preliminary experiments in another 10 Kunming mice. All the sinus node, atrial myocardium, and ventricular myocardium tissue from 180 mice were dissected and frozen by liquid nitrogen. The structure of tissue was observed by HE staining. Total RNA were extracted and quality-controlled before hybridize with miRNA chip. The chips with miRNA were used to screen specific miRNAs; and correlation analysis of gene function was done. ResultsThe area of mice sinus node located at juncture of the superior vena cava and the right atrium junction with crista as its longitudinal axis, ranged 2.0 mm×1.5 mm×1.0 mm. HE staining showed the sinus cells were less, with no stripes, lightly stained cytoplasm, large and round nucleus, and there were much fibrous connective tissue around cells with a visible sinus node artery. The miRNA microarray results showed that compared with atrial myocardium and ventricular myocardium, there were 39 differentially expressed miRNAs in sinus node, including 12 up-regulated miRNAs and 27 down-regulated miRNAs. Based on the regulatory networks of differential miRNA and target gene, the regulatory miRNA was obtained. ConclusionThe differentially expressed miRNA in mice sinus node possibly may be involved in the regulation of sinus cell differentiation.