目的报告Vacuseal负压引流系统在腹部外科的应用体会。方法1999年5月至2002年3月，作者对47例腹部手术病例实施Vacuseal引流，其中肝切除术6例，急性胰腺炎胰床松解术7例，胰十二指肠切除术5例，门奇断流术4例，腹腔清创术4例，直、结肠癌根治术10例，切口感染11例。并与同期64例（次）普通负压引流结果进行比较。结果Vacuseal组日均腹腔引流量大于普通引流，引流后伤口肉芽组织情况改善，二期缝合时间缩短，无引流不适反应。结论Vacuseal负压引流的效果明显优于普通负压引流，适用于腹部的脏器切除或严重炎症、低位空腔脏器术后、巨大缺损的伤口感染。
To study the effect of autogeneic PRP on prol iferation and osteogenetic differentiation of human adipose-derived stem cells (ADSCs) in vitro. Methods ADSCs were isolated from adipose tissue obtained from donor undergoing l iposuction and were cultured, and growth condition of the cells was observed by inverted microscope. ADSCs at passage 3 were cultured in adipogenic or chondrogenic medium and underwent identification, immunofluorescence staining observations for CD29 and CD44 were performed. ADSCs at passage 3 were divided into 2 groups: PRP group cultured by osteogenic induction culture medium containing 10 mL/L PRP, and control group cultured by osteogenic induction culture medium without PRP. Then growth condition of the cells was observed by inverted microscope. MTT method was used to observe cell prol iferation activity 1, 2, 3, 4 and 5 days after culture. ALP activity detection was conducted 7, 14, 21 and 28 days after culture. ALP staining was performed on PRP group 7 and 14 days after culture. Al izarin red staining was performed on PRP group 14 days after culture to detect the formation of calcium nodule. Results Under the inverted microscope, most ADSCs at passage 3 were spindle-shaped and the doubl ing time was about 35 hours. Adipogenic and chondrogenic differentiation were confirmed, and the cells were positive for CD29 and CD44 immunofluorescence staining. MTT method revealed the absorbance value of PRP group at 1, 2, 3, 4 and 5 days was 0.137 ± 0.015, 0.219 ± 0.023, 0.367 ± 0.031, 0.586 ± 0.039 and 0.948 ± 0.046, respectively, and in the control group, it was 0.081 ± 0.009, 0.115 ± 0.012, 0.162 ± 0.017, 0.242 ± 0.025 and 0.356 ± 0.032, respectively, suggesting there were significant differences between two groups (P lt; 0.01). At 7 days after osteogenic induction, PRP group was positive for ALP staining, grey-black cell plasm and black precipitate were evident; the positive cells increased