west china medical publishers
Author
  • Title
  • Author
  • Keyword
  • Abstract
Advance search
Advance search

Search

find Author "余少鸿" 5 results
  • ethods of Prevention and Treatment of Severe Complications after Pancreatoduodenectomy

    目的 探讨胰十二指肠切除术后严重并发症的防治方法。方法 分析2002~2005年7例胰十二指肠切除术后严重并发症的资料。结果 手术严重并发症发生率为25.9%(7/27),经积极治疗后无一例死亡。结论 术中仔细操作和操作技术改进是降低胰十二指肠切除术并发症发生率的关键,并发症经积极治疗后有望痊愈。

    Release date: Export PDF Favorites Scan
  • 高龄患者结直肠手术后吻合口漏的预防及处理

    目的探讨高龄患者结直肠手术后吻合口漏的预防及处理措施。 方法回顾性分析2010年1月至2014年12月期间收治的125例老年(≥70岁)结直肠手术后发生吻合口漏患者的临床资料。 结果本组发生吻合口漏12例,发生率为9.6%(12/125)。2例经结肠造瘘痊愈;余10例通过抗感染、营养支持、通畅引流等保守治疗痊愈,临床治疗时间为27~126 d,平均46.2 d;治疗费用为4.6~27.0万元,平均12.6万元。 结论高龄患者结直肠手术后吻合口漏可根据术中情况采取有效预防措施,针对临床表现予具体处理,手术方式以简单有效为宜。

    Release date: Export PDF Favorites Scan
  • Construction of mdr1 Expression Vector and Detection of Its Expression in HepG2 Cells

    【Abstract】ObjectiveTo construct an mdr1 expression vector and detect its expression in HepG2 cells in vitro. MethodsThe 4.5-kb mdr1 cDNA was obtained from the plasmid pHaMDR1 cloned into the PCIneo mammalian expression vector, which was later transferred into human hepatocarcinoma cell line HepG2 by liposome. Then the HepG2 cells resisting G418 were clustered and proliferated,and the specific fragment of mdr1 cDNA, mRNA and the Pgp in these HepG2 cells were detected by means of PCR, RT-PCR and FCM respectively. ResultsThe mdr1 expression vector was constructed successfully,and the stable multidrug resistance(MDR) hepatocarcinoma cell line (HepG2/mdr1) was developed as well. The outcome of PCR analysis showed that the specific fragment of mdr1 cDNA could be found in HepG2/mdr1 cells, but not in the nontransfection HepG2 cells. Furthermore,the content of the specific fragment of mdr1 mRNA and the expression of P-gp in HepG2/mdr1 cells were (59.7±7.9)% and (12.5±5.45)% respectively, the corresponding value in HepG2 cells were (16.9±3.2)% and (4.63±2.59)% respectively. The difference was statistically significant (P<0.05). ConclusionIt is praticable to develop MDR hepatocarcinoma cell line by transferring mdr1 cDNA into HepG2 cells, which is useful in the research of MDR mechanism.

    Release date:2016-09-08 11:54 Export PDF Favorites Scan
  • Expression and Significance of PLK1 and STK15 Gene, and Effect of Its Specific Inhibitor on Proliferation in Colon Cancer Cells

    ObjectiveTo explore the expressions of polo-like kinase 1(PLK1) and serine/threonine kinase 15 (STK15) mRNA and protein in colon cancer cells, and to explore the inhibitive effect of SBE13 and VX-680 for PLK1 protein and STK15 protein. MethodsOne kind of cervical cancer cells(Hela cells) and 3 kinds of colon cancer cells (HCT-116 cells, HT-29 cells, and CACO-2 cells) were selected for experiment. Expression levels of PLK1 mRNA, STK15 mRNA and its protein of 4 kinds of cells were detected by reverse transcription polymerase chain reaction(RT-PCR) and Western blot method respectively. Inhibitive effect of SBE13 and VX-680 were evaluated in vitro by methylthiazolyldiphenyl-tetrazolium bromide(MTT) assay in 4 kinds of cells, which divided into 5 groups, receiving Dulbecco's modification of Eagle's medium(DMEM), dimethylsulfoxide(DMSO), SBE13, VX-680, and SBE13+VX-680 respectively. ResultsCompared with Hela cells, expression levels of PLK1 mRNA, STK15 mRNA and its protein in HCT-116 cells,HT-29 cells, and CACO-2 cells were higher(P<0.05). ① Hela cells:Compared with DMEM group, the proliferative activity were not inhibited in SBE13 group, VX-680 group, and SBE13+VX-680 group(P>0.05). ② HCT-116 cells and HT-29 cells:Compared with DMEM group, the proliferative activity were inhibited in VX-680 group and SBE13+VX-680 group(P<0.05), but was not inhibited in SBE13 group(P>0.05). ③ CACO-2 cell:Compared with DMEM group, the proliferative activity were inhibited in SBE13 group, VX-680 group, and SBE13+VX-680 group(P<0.05). ConclusionsExpression levels of PLK1 mRNA, STK15 mRNA and its protein increase in HCT-116, HT-29, and CACO-2 cells compared with Hela cells. SBE13 and VX-680 can inhibit PLK1 and STK15 protein partly in colon cancer cell lines.

    Release date: Export PDF Favorites Scan
  • Value of Ultrasound-Guided Trans-Hepatic Bile Tract Exploration in Treatment of Hepatolithiasis

    目的 探讨与评价术中B超定位下经肝胆管取石在肝内胆管结石治疗中的手术指征及优劣性。方法 总结2002~2006 年29例肝内胆管结石行肝叶切除+经肝胆管取石患者的临床资料,对其手术效果及并发症进行分析。结果 无胆管损伤及手术死亡病例, 并发症发生率为37.93%,残石率为10.34%。结论 术中B超定位下经肝胆管取石结合肝叶切除对肝内胆管结石是一种较好的治疗方式,主要适用于Ⅱb型肝内胆管结石患者。

    Release date:2016-09-08 11:07 Export PDF Favorites Scan
1 pages Previous 1 Next

Format

Content