ObjectiveThe changes of intestinal permeability and relationship of intestinal mucosa and bacterial translocation were studied in rat acute necrotizing pancreatitis (ANP) models.MethodsThe ANP models were made by injection of 5% sodium taurocholate 1.0 ml into pancreatic subcapsula.Then wistar rats were divided into four groups,control group (n=20),ANP group(n=22),treatment model group fed with lactose (n=22) and treatment model group fed with MgSO4 and antibiotic (n=22).After 72 hours,the experimental models were sacrificed.Tissues of pancreas,mesenteric lymph node, ascites were collected for microbiological study.The intestinal permeability was observed by lanthanum tracer.The blood samples were obtained from portal vein and ascites in order to assay the amount of amylase in serum.The pathologic lesions were found in the intestinal villus of the model group, including acute necrosis of intestinal mucosa,necrotichaemorrhage as well as enteroparalysis and a mass of haemorrhagic ascites.ResultsBacterial translocation of model group were markedly elevated than that of control (P＜0.05).There were statistically significant differences in bacterial translocation among three model groups （P＜0.05）.The pathologic lesions were found in the intestinal villus of the model group,including acute necrosis of intestinal mucosa,necrotichaemorrhage as well as enteroparalysis and a mass of haemorrhagic ascites.The lanthanum grain in clearance of intestinal cell of model group can be observed by eletron microscope.ConclusionThere is a severe gut barrier damage and injury in the intestinal mucosa,which lead to bacterial translocation from intestine as the source of pancreatic infection.Cleaning out enteric bacteria,improving intestinal movement and feeding with lactose could decrease bacterial translocation to treat and prevent acute necrotizing pancreatitis.
目的 总结活体部分小肠移植在治疗短肠综合征合并肠瘘中的临床经验。方法 1例短肠综合征合并肠瘘患者接受其子的150 cm 回肠，供肠动、静脉分别与受体的腹主动脉和下腔静脉行端侧吻合，受体残余空肠与供体回肠近端行端端吻合，受体结肠与供肠远端行端侧吻合，供肠远端造瘘作为观察窗，术后给予免疫抑制等治疗。 结果 患者小肠移植术后恢复顺利，肠道功能恢复，血管吻合口通畅，正常生活110 d后因心脏意外死亡。结论 短肠综合征合并肠瘘患者实施活体部分小肠移植是可行的，植入肠管的血管植入技术对小肠移植成功非常重要。