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巩膜外植物脱出的原因和处理

胃癌脉络膜转移一例

逆行足底内侧皮瓣修复前足底皮肤软组织缺损

目的 探讨足底内侧皮瓣逆行运用修复前足底皮肤软组织缺损的临床效果。 方法 2004年3月-2009年3月，对11例前足皮肤软组织缺损病例采用逆行足底内侧皮瓣转移修复，观察其成活、外观及功能。 结果 11例皮瓣全部成活，随访3～6个月，皮瓣外观满意、耐磨耐压性能好。 结论 逆行足底内侧皮瓣修复前足底皮肤软组织缺损操作简便，疗效可靠。

Effects of Hydroxysafflor Yellow A on Microvessel Density and mRNA Expression of Vascular Endothelial Growth Factor in Mice Transplanted Lewis Lung Cancer

ObjectiveTo observe the effects of hydroxysafflor yellow A (HSYA) on microvessel density (MVD) of mice transplanted Lewis lung cancer and mRNA expression of vascular endothelial growth factor (VEGF) so as to explore the tumor-inhibiting mechanism of HSYA. MethodsSixty tumor-bearing C57/BL mice were randomly divided into five groups, with 12 mice in each group, namely a control group, a cyclophosphamide (CTX) group (25mg/kg), a large dose HSYA group (112mg/L), a medium dose HSYA group (56mg/L), and a small dose HSYA group (28mg/L). These different drugs were administered by intraperitoneal injection. The mice were sacrificed 22 days after the treatment. Tumor tissues were sampled and examined by immunohistochemical method and quantitative real-time PCR to detect the expression of MVD and VEGF mRNA. ResultsThe MVD of the medium and small dose HSYA groups and CTX group were 30.01±3.12, 22.56±2.11 and 16.21±2.40, respectively, which were significantly lower than 41.10±2.93 of the control group and 37.66±3.04 of the large dose HSYA group (χ2=2.82, P=0.010;χ2=3.16, P=0.007;χ2=4.58, P=0.000) and (χ2=1.98, χ2=0.038;χ2=2.45, P=0.016;χ2=3.82, P=0.001). The difference in VEGF amplified fluorescence expression threshold between the HSYA groups and the control group was not significant. However, after amplification, the expression of VEGF mRNA in the small dose HSYA group was only 0.43±0.16, which was obviously lower than 0.82±0.06 in the control group (F=0.77, P=0.038). ConclusionHSYA can significantly reduce MVD in mice transplanted Lewis lung cancer and down-regulate expression of VEGF mRNA to achieve tumor-inhibiting effect.