In order to observe the effect of hepatocyte growth promoting factor (pHGF) on liver regeneration of rat with cirrhosis after hepatectomy, IBAS Ⅱ auto image analysis technology was used to measure the variety of DNA ploid rate of hepatocytes and OPTDM of enzymes by liver histochemistry after hepatectomy; serum levels of the glutamicpyruvic transaminase (SGPT) and indocyanine green retention rate in 15 minute (ICG15) were tested to measure the function of the remanent liver. The results revealed that tetraploid hepatocytes lowered greatly and diploid, quintploid and ＞quintploid hepatocytes increased apparently in group A. OPTDM of enzymes by liver histochemistry showed no significant difference at the first day after operation in each group (Ｐ＞0.05); SDH and LDH of group A were significantly higher than those of group B and AkP, AcP were significantly lower at the second or fifth day after hepatectomy. Serum tests showed that SGPT, ICG15 of group A decreased apparently at the fifth day after operation. The results demonstrate that pHGF not only stimulates the regeneration of the remanent liver but also accelerates the functional mature of the regenerative hepatocytes and the functional recovery of the remanent liver after resection of cirrhotic liver of rats.
ObjectiveTo investigate effect of small interfering RNA (siRNA) targeting inhibition of growth hormone receptor (GHR) on proliferation and invasion of human liver cancer line SMMC-7721. MethodsSMMC-7721 cells were transfected with siRNA targeting human GHR by GenMuteTM transfection regent.The cells were divided into three groups:blank control group (non-transfected siRNA),negative control group (transfected with non-specific siRNA),and specificity transfected group (transfected with expression specifically interfere by GHR siRNA).the relative expression of GHR mRNA was detected by using real-time PCR.the expression of GHR protein was detected by using Western blot.The cell proliferation was assessed by CCK-8 assay.And the ability of invasion was examined by Transwell assay. ResultsThe expressions of GHR mRNA and GHR protein in the specificity transfected group were significantly lower than those in the blank control group (P<0.05) and the negative control group (P<0.05).Compared with the blank control group and the negative control group,the absorbance value and the number of migrating cells of SMMC-7721 cells were decreased obviously (P<0.05) in the specificity transfected group. ConclusionsiRNA targeting human GHR could reduce capability of proliferation migration and invasion of SMMC-7721 cells.
ObjectiveTo investigate the diagnostic value of endoscopic retrograde cholangiopancreatography (ERCP) in obstructive jaundice of elderly patients. MethodsTotally 338 patients with obstructive jaundice underwent ERCP were divided into elderly group (age ≥60 years old) and nonelderly group (age lt;60 years old) based on age. The levels of serum amylase (AMY), ALT, and TBIL in 6, 24, and 48 h after ERCP were detected. The success rate of cholangiopancreatography, accuracy rate of diagnosis, and incidence of complications after ERCP in two groups were analyzed. ResultsThe difference of serum AMY, ALT, and TBIL levels of patients in 6, 24, and 48 h after ERCP were not significant between two groups (Pgt;0.05). The success rate of cholangiopancreatography in nonelderly group was 96.3% (130/135) and in elderly group was 96.1% (195/203), and no difference was found (Pgt;0.05). However, the accuracy rate of diagnosis of ERCP in nonelderly group (84.6%, 110/130) was significantly lower than that in elderly group (98.5%, 192/195), Plt;0.05. The difference of the incidence of complications was not significant between two groups 〔14.8% (20/135) vs. 17.2% (35/203)〕, Pgt;0.05. There was no mortality in two groups patients. ConclusionERCP is a safe, effective, and accurate method, which is of importance to the diagnosis of obstructive jaundice in elderly patients.
ObjectiveTo systematically review the efficacy and safety of hemihepatic blood flow occlusion versus Pringle's maneuver during hepatectomy. MethodsWe electronically searched The Cochrane Library (Issue 8, 2013), PubMed, EMbase, CBM, CNKI, VIP and WanFang Data for randomized controlled trials (RCTs) about hemihepatic blood flow occlusion versus Pringle's maneuver during hepatectomy. The duration of search was from the inception of the databases to August 2013. Meanwhile, references of the included studies were also retrieved. After literature selection, data extraction and quality assessment conducted by two reviewers independently, meta-analysis was conducted using RevMan 5.2 software. ResultsSeven studies involving 624 patients were finally included. The results of meta-analysis showed that: a) for safety, Pringle's maneuver was shorter than hemihepatic blood flow occlusion in operation time (SMD=0.34, 95%CI 0.02 to 0.66, P=0.04). But they were alike in intraoperative blood loss, transfusion requirements, hospitalization time, and complications. b) For effectiveness, Pringle's maneuver was lower than hemihepatic blood flow occlusion in the levels of 3rd day ALT (SMD=-0.71, 95%CI-1.28 to-0.14, P=0.02), 7th day ALT (SMD=-1.73, 95%CI-2.85 to-0.62, P=0.002), 1st day AST (SMD=-0.74, 95%CI-1.38 to-0.09, P=0.03), 7th day AST (SMD=-0.99, 95%CI-1.26 to-0.71, P < 0.000 01), 3rd day TBIL (SMD=-0.34, 95%CI-0.57 to-0.10, P=0.005), and 7th day TBIL (SMD=-0.52, 95%CI-1.02 to-0.01, P=0.04). ConclusionCompared to the Pringle's maneuver, hemihepatic blood flow occlusion during hepatectomy could promote the recovery of liver function. However, the number of the included RCTs in the review is small and the quality is low, some of the main information is not reported and the information for analysis lacks. Therefore, the aforementioned conclusion needs to be verified by conducting more large-scale, multicenter and high-quality RCTs.