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find Author "吴明营" 2 results
  • 右心室双通道术治疗右心室流出道狭窄

    摘要: 目的 探讨先天性右心室流出道狭窄合并冠状动脉横跨右心室流出道表面患者行右心室双通道术的方法及临床效果。 方法 2006年4月至2008年8月,我中心连续收治6例右心室流出道狭窄合并冠状动脉横跨右心室流出道表面患者, 男4例,女2例;年龄0.8~12.0岁,平均年龄5.5岁。法洛四联症2例,右心室双出口4例。手术在保留原来右心室肺动脉出口的同时,采用自体心包片或国产牛心包片建立右心室的第2个出口。 结果 围术期无死亡患者,术后右心室与肺动脉压差为24.17±6.18 mm Hg。出院前复查心脏超声心动图提示:2个出口均有血液,未见流出道阻塞。随访6例,随访时间3个月~1年,其中4例患者心前区闻及2/6级收缩期杂音,2例闻及3/6级杂音。超声心动图提示:所建的右心室通道通畅,右心室与肺动脉间的压差(24.67±5.32 mm Hg)与术后早期比较变化不大,远期效果尚待进一步观察。 结论 右心室双通道术可在保护横跨右心室流出道表面冠状动脉的情况下疏通右心室流出道。

    Release date:2016-08-30 06:02 Export PDF Favorites Scan
  • The Differentially Expressed Cytokines of Peripheral Blood Mononuclear Cell after Cardiopulmonary Bypass

    Objective To determine the application values of gene chip technique in cardiovascular surgical clinical and research work. Microarray for gene expression profiles was used to screen out the differentially expressed genes during cardiopulmonary bypass(CPB) in peripheral blood mononuclear cell. By doing these, it was hoped that some clues in inflammatory response during CPB could be found out. Methods The patients’ oxygenated bloods were drawn immediately before onset and termination of CPB. Peripheral blood mononuclear cell (PBMC) were obtained from heparinised blood by Ficoll gradient centrifugation. The differentially expression was measured using BD AtlasTM cDNA Expression Arrays. The candidate genes were corroborated by semiquantitative reverse transcriptionpolymerase chain reaction (RT-PCR). Results Gene chip technique was successfully used in CPB study. The gene expression profiles of cytokines of PBMC during CPB were screened out. Interleukin 6 and Wnt5a were the differentially expressed genes. But the validity using semiquantitative RT-PCR found no statistically difference(P=0.888,0.135). Conclusion Microarray technique has positive application values in the study of cytokines during CPB. cDNA microarray for gene expression profiles can primarily screen out differentially expression genes during CPB. These genes may be engaged in inflammation and other pathophysiological reactions during CPB. PBMC is not the major source of cytokines during CPB.

    Release date:2016-08-30 06:09 Export PDF Favorites Scan
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