目的探讨对达芬奇机器人纵隔肿瘤切除术的患者实行心理干预有无积极的影响。 方法选取沈阳军区总医院胸外科2012年1月至2013年1月行达芬奇机器人纵隔肿瘤切除术患者40例为研究对象，按就诊顺序分为两组。对照组20例，男11例、女9例，平均年龄43.7（32～70）岁；观察组20例，男10例、女10例，年龄45.6（32～68）岁。两组均按照胸外科常规护理，观察组在此基础上加强围手术期的心理干预，使用焦虑自评量表（self-rating anxiety scale，SAS）和抑郁自评量表（self-rating depression scale，SDS）进行评估，比较两组患者干预前后的心理状态差异。 结果观察组和对照组SAS、SDS评分干预前与干预后均有不同程度的降低。与对照组相比，观察组降低较为明显，且差异有统计学意义（SAS干预后44.55±5.33 vs.39.90±2.34，P＜0.05；SDS干预后35.64±4.26vs.33.28±1.93，P＜0.05）。 结论心理干预可有效改善达芬奇机器人纵隔肿瘤切除术患者的心理状态，对提高治疗效果有积极的推动作用。
ObjectAimed to describe the clinical characteristics of the patients with interictal schizophrenia-like psychoses of epilepsy (SLPE), so as to improve the identification, diagnosis and treatment.MethodsWe collected the cases from January 2017 to December 2019 that diagnosed as "epileptic psychosis/organic mental disorders/brain damage and functional disorders and somatic diseases caused by other mental disorders/organic delusions (schizophrenia-like) disorders" in the medical record system of the Sixth Hospital of Changchun. The discharge records were re-diagnosed by two experienced epilepsy specialists and psychiatrists respectively. Retrospective statistical analysis was performed on the cases identified as SLPE.ResultsA total of 45 patients were diagnosed as SLPE (male: female=1:1.4). The onset age of epilepsy and mental symptoms was (16.4±12.5) years and (35.3±13.4) years respectively. The duration of mental symptoms after first seizure was (18.9±13.4) years. 7 patients (15.6%) were not treated with AEDs, and 26 patients (57.8%) were treated with first generation AEDs. 8 patients (17.8%) had no seizures within 1 year before the onset of mental symptoms, and 28 patients (62.2%) had frequent seizures, even status epilepticus or clustered seizures. 2 patients (4.4%) had generalized tonic-clonic seizure, only 4 patients (8.9%) showed focal impaired awareness seizure, and 39 patients (86.7%) had focal to bilateral tonic-clonic seizure.The PANSS positive symptom score, PANSS negative symptom score and BPRS score were (15.1±4.4), (17.7±4.6) and (44.7±8.4) respectively.ConclusionThere were some features of epilepsy in SLPE, such as early onset age, frequent seizure (some patients were seizure-free), focal epilepsy, and poor AEDs treatment compliance. The onset age of mental symptoms in SLPE was later than Schizophrenia and long duration after first seizure. The PANSS scale showed that the mental symptoms of patients with SLPE were similar to those of patients with schizophrenia, and both positive and negative symptoms existed.
ObjectiveTo explore the effect mechanism of rehabilitation therapy post incomplete spinal cord injury (ISCI) in the view of graph theoretic analysis of the whole brain regions by the means of resting state functional magnetic resonance imaging (rs-fMRI).MethodsPatients with ISCI admitted to the Department of Rehabilitation Medicine of Tsinghua University Affiliated Beijing Tsinghua Changgung Hospital from January 2017 to June 2020 were selected and healthy subjects recruited in the same period were also selected. The patients were given comprehensive rehabilitation treatment for 2 weeks, including physical therapy, functional electrical stimulation treadwheel, walking training, etc. Healthy subjects and patients before and after treatment course were examined by rs-fMRI. While patients were assessed using the motor and sensory scores of American Spinal Injury Association (ASIA), muscle tone assessment, pain assessment, Walking Index for spinal cord injury (WISCI) as well as the Spinal Cord Independence Measure (SCIM).ResultsA total of 23 ISCI patients and 22 healthy subjects were included. After 2 weeks of treatment, ASIA lower limb motor function scores (P<0.001), ASIA sensory scores (P=0.005), Visual Analogue Scale (VAS) (P=0.027), WISCI (P=0.005) and SCIM (P<0.001) scores of the patients were significantly improved compared with before treatment. Before treatment, compared with healthy subjects, ISCI patients had lower betweenness centrality (BC) in the brain regions of R38, lower local efficiency (LE) in L21, L22, L39, L41, L42, L44, L48, R22, R39 and lower weighted degree (WD) in L22, L39, L41, L44, L48, R22, R39. After treatment, compared with the healthy subjects, the BC of R5, R6, R7, AR111 and AL112 of ISCI patients increased and in R6 and AR94, the clustering coefficient increased. The LE and WD of L21 and R21 in ISCI patients after treatment were higher than those before treatment.ConclusionsThe functional analysis of the whole brain network based on graph analysis can sensitively reflect the changes of brain network remodeling in patients with ISCI. Spinal cord injury can cause the decline of graph theoretical attributes of the auditory center-related brain regions. After rehabilitation treatment, the sensorimotor cortex, auditory center and extravertebral brain regions information transmitting ability in the whole brain network are improved, suggesting that rehabilitation training may participate in brain function remodeling by activating the sensorimotor center and non-motor center-related brain regions.
Objective To analyze the research hotspots and development trends of hospital scientific research management in China, and to provide references for the innovation and development of hospital scientific research management in China. Methods The CNKI database was electronically searched to collect relevant research on scientific research management of domestic hospitals from January 1981 to September 2022. Bibliometrics and CiteSpace software were used for analyze. Results A total of 2 688 articles were included. The trend of publishing hospital scientific research management literature showed four peaks. The institution with the most publications was Peking University People's Hospital. Six authors published more than 15 articles. The most concerned was clinical research. Public hospitals, scientific research projects, and refined management were the latest emerging words. Conclusion The scientific research management of hospitals in China has formed an academic exchange group, but the core author group is small, and there is a lack of cross-institutional cooperation. The innovation mode of scientific research management shows a good development trend, the quantitative index system of scientific research evaluation at all levels is gradually improved, the ability to identify the potential of scientific research talent has been improved, and the cultivation of scientific research management teams needs to be strengthened.
目的探讨术后综合干预在达芬奇机器人手术系统行纵隔肿瘤切除术患者预后上的优势。 方法选取2013年10月至2015年2月沈阳军区总医院行达芬奇机器人手术系统纵隔肿瘤切除术94例，随机分为干预组和对照组，每组各47例。对照组按胸外科一般常规予围手术期治疗与护理，干预组在此基础上实施针对此手术制定的综合术后干预策略。分析比较两组患者临床效果指标及生活质量。 结果干预组和对照组术后监护时间[（27.83±6.64）d vs.（55.85±14.97）d，P=0.000]、术后留置引流管时间[2（0～6）d vs.5（0～9）d，P=0.000]、术后疼痛评分（0.89±0.70 vs.2.83±0.92，P=0.000）、术后住院时间[（4.85±1.49）d vs.（8.11±1.94）d，P=0.000]、SAS评分（48.43±3.82 vs.57.66±3.42，P=0.000）、生活质量评分（38.06±3.24 vs.31.83±2.44，P=0.000）差异均有统计学意义。 结论术后综合干预加速了达芬奇机器人手术系统行纵隔肿瘤切除术术后患者的康复，提高了患者的生活质量。
ObjectiveTo investigate the effects of hypoxia-inducible factor 1α (HIF-1α) small interfere RNA construct pSUPERH1-siHIF1α on the expression of CD18 and ninjurin-1 by K562 (human chronic myelogenous leukemia cell line) cells cultured with serums from patients with early stage of diabetic retinopathy. MethodsK562 cells were cultured in 4 groups as control group (group A), diabetic group (group B), diabetes and pSUPERH1-siHIF1α transfect group (group C) and diabetes and pSUPER-retro transfect group (group D). The cells in group A were cultured in human serum from age-matched healthy control, and in group B, C and D, the cells were cultured in serum from the subjects of early stage of diabetic retinopathy. Twenty-four hours before the cells were cultured by the serum from the subjects of early stage of diabetic retinopathy, the HIF-1α specific siRNA expression vector pSUPERH1-siHIF1α and empty vector pSUPER-retro were transfected into the cells of group C and D, respectively. The percentages of CD18 and ninjurin-1 positive cell on the surface of K562 cells were measured by Flow Cytometry. The adherent rate between K562 and RF/6A was measured by the rose Bengal staining test. ResultsThe percentages of CD18 positive cell in the group A, B, C and D were significantly different (F=14.33, P=0.01). The percentage of group B was significantly higher than that in group A (P=0.001); the percentage of group C was significantly lower than that in group B (P=0.001) and group D (P=0.02); the difference between group C and A was not significant (95%CI=-14.89-2.13, P=0.12). The differences of the percentage of ninjurin-1 positive cell among the group A, B, C and D were significant (F=39.38, P=0.001). The percentage of group B was significantly higher than that in group A (P=0.00); the difference of the percentage between group C and B was not significant (P=0.06), that was also not significant between group C and D (P=0.49). The differences of the adherent rate between K562 and RF/6A (rhesus monkey retinal choroid blood vessel endothelial cell line) among the group A, B, C and D were significant (F=20.62, P=0.00). The adherent rate of group B was significantly higher than that in group A (P=0.00), the adherent rate in group C was significantly lower than that in group B (P=0.01), but it was still significantly higher than that in group A (P=0.002), the difference of adherent rate between group B and D was not significant (P=0.68). ConclusionUnder the early stage of diabetic retinopathy, HIF-1α small interfere RNA pSUPERH1-siHIF1α may significantly suppress the expression of CD18 on the surface of K562 cells, but it may not significantly influence the expression of ninjurin-1 on the surface of K562 cells.
Alzheimer’ s disease is the most common kind of dementia without effective treatment. Via early diagnosis, early intervention after diagnosis is the most effective way to handle this disease. However, the early diagnosis method remains to be studied. Neuroimaging data can provide a convenient measurement for the brain function and structure. Brain structure network is a good reflection of the fiber structural connectivity patterns between different brain cortical regions, which is the basis of brain’s normal psychology function. In the paper, a brain structure network based on pattern recognition analysis was provided to realize an automatic diagnosis research of Alzheimer’s disease and gray matter based on structure information. With the feature selection in pattern recognition, this method can provide the abnormal regions of brain structural network. The research in this paper analyzed the patterns of abnormal structural network in Alzheimer’s disease from the aspects of connectivity and node, which was expected to provide updated information for the research about the pathological mechanism of Alzheimer’s disease.
To establ ish a simple and stable cervical ectopic renal transplantation rat model that increase surgical successful rate. Methods A total of 208 male inbred Wistar rats (weighing 220-260 g) were randomly served as donors and recipients. The graft consisting of kidney, renal vein (RV) and renal artery (RA) was obtained, and perfused in situ. The donor RA was end-to-end anastomosed to the recipient left common carotid artery (CCA) by using of “sleeve” anastomosis,and the donor RV to the recipient right external jugular vein by using of “cuff” technique. The distal end of the ureter wasbrought out to form cervical cutaneous stomas. Results A total of 104 ectopic renal transplantations were performed in rats, including stages of the pre-experiment (62 operations) and experiment (42 operations). The success rates of the two stages were 80.6% and 95.2%, respectively. The causes of failure in the pre-experimental stage were anesthesia accidents, thrombosis of the arterial anastomosis, massive hemorrhage, air embol ism and phlebemphraxis. In the experimental stage, 2 rats died due to late anastomotic hemorrhage and thrombosis. The remaining 40 transplanted kidney survived more than 6 months. The time for surgery was (40 ± 6) minutes, the average time for donor surgery was (20 ± 5) minutes, the preparation time for the graft was (8 ± 2) minutes, the operative time for the recipient was (18 ± 3) minutes, including the time for the arterial anastomosis (5 ± 2) minutes and venous anastomosis (2 ± 1) minutes, the cold ischemia time of graft was (15 ± 3) minutes. Conclusion The cervical ectopic renal transplantation technique has the advantages of easy-and fast-to-perform, shorter operation and cold ischemia time, higher successful rate.
ObjectiveTo summarize the efficacy of robot-assisted thoracic surgery (RATS) and video-assisted thoracoscopic surgery (VATS) in the treatment of left upper lobectomy for non-small cell lung cancer. MethodsThe clinical data of patients with non-small cell lung cancer who underwent left upper lobectomy with RATS or VATS in our center from January 2019 to October 2021 were retrospectively analyzed. The patients were divided into two groups according to surgical methods: a RATS group and a VATS group. The baseline clinical data and results were compared between the two groups. ResultsA total of 145 patients were included. There were 78 males and 67 females with a mean age of 59.9 years. There were 63 patients in the RATS group and 82 patients in the VATS group. There was no death within 30 days after operation in both groups. In the RATS group, the drainage volume on the second postoperative day (233.49±83.94 mL vs. 284.88±120.21 mL, P=0.003), total operative time (126.94±29.50 min vs. 181.59±61.51 min, P=0.000), intraoperative resection time of the left upper lobe (76.48±27.52 min vs. 107.23±47.84 min, P=0.000), intraoperative blood loss (P=0.000), and conversion rate to thoracotomy (P=0.018) were significantly better than those in the VATS group. The group (5.41±0.94 groups vs. 4.83±1.31 groups, P=0.002) and number (18.27±7.39 vs. 12.76±6.54, P=0.000) of dissected lymph nodes in the RATS group were significantly more than those in the VATS group. The differences in the drainage volume on the first day after operation, postoperative intubation time, postoperative hospital stay or postoperative complications between the two groups were not statistically significant (P>0.05). ConclusionThe application of RATS in the left upper lobectomy for non-small cell lung cancer is safe and feasible, and has obvious advantages over VATS.
Objective To analyze the risk factors for postoperative length of stay (PLOS) after mediastinal tumor resection by robot-assisted non-endotracheal intubation and to optimize the perioperative process. MethodsThe clinical data of patients who underwent Da Vinci robot-assisted mediastinal tumor resection with non-endotracheal intubation at the Department of Thoracic Surgery, General Hospital of Northern Theater Command from 2016 to 2019 were retrospectively analyzed. According to the median PLOS, the patients were divided into two groups. The univariate analysis and multivariate logistic regression were used to analyze risk factors for prolonged PLOS (longer than median PLOS). ResultsA total of 190 patients were enrolled, including 92 males and 98 females with a median age of 51.5 (41.0, 59.0) years. The median PLOS of all patients was 3.0 (2.0, 4.0) d. There were 71 patients in the PLOS>3 d group and 119 patients in the PLOS≤3 d group. Multivariate logistic regression showed that indwelled thoracic catheter [OR=11.852, 95%CI (2.384, 58.912), P=0.003], preoperative symptoms of muscle weakness [OR=4.814, 95%CI (1.337, 17.337), P=0.016] and postoperative visual analogue scale>5 points [OR=6.696, 95%CI (3.033, 14.783), P<0.001] were independent factors for prolonged PLOS. Totally no tube (TNT) allowed patients to be discharged on the first day after surgery. ConclusionRobot-assisted mediastinal tumor resection with non-endotracheal intubation can promote rapid recovery. The methods of optimizing perioperative process are TNT, controlling muscle weakness symptoms and postoperative pain relief.