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find Author "吴硕东" 14 results
  • Application of near infra-red fluorescence imaging in biliary surgery

    ObjectiveTo summarize the research progress of near infra-red fluorescence imaging (NIRFI) in biliary tract surgery, and to provide protection for improvements of therapeutic effect and safety of biliary tract surgery.MethodThe relevant literatures about studies on NIRFI in the biliary tract surgery in recent years were reviewed.ResultsThe NIRFI had been preliminarily used in the surgical treatment of benign and malignant biliary diseases, and had shown its unique value in cholangiography. It provided a new method for effectively avoiding surgical complications, shortening operation time, reducing the rate of conversion to open surgery, evaluating blood supply of bile duct and improving the safety of operation.ConclusionsNIRFI has achieved notable successes in treatment of biliary tract diseases. With future application of fluorescence imaging in near infra-red Ⅱ window and new specific fluorescence targeting molecules, this technique will highlight its more important values in biliary surgery.

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  • Operative Techniques of Single Incision Laparoscopic Surgery in Treatment of Colorectal Neoplasm

    Objective To summarize the experience of single incision laparoscopic colorectal surgery and to discuss the operative techniques. Methods The clinical data of 21 cases who underwent single incision laparoscopic colorectal surgery in Shengjing Hospital from Jan. 2010 to Jun. 2011 were collected and analyzed. Results Of 21 cases underwent single incision laparoscopic surgery, right hemicolectomy performed in 5 cases, sigmoidectomy performed in 2 cases, rectal anterior resection performed in 9 cases, rectal abdominoperineal resection performed in 2 cases, total colectomy performed in 1 case, and colostomy performed in 2 cases. Twenty cases completed by single incision, but 1 case was added an extra 12 mm incision in order to dissect the lower segment of rectum. The operative time was (189±75) min (40-335min);the postoperative hospitalization time was (11.5±3.4) d (7-16d). There were no bleeding, anastomosis leakage or intestinal obstruction after operation, and no incision infection, rupture or hernia were founded. No recurrence was found within 6 months’ follow up after operation. Conclusions Under reasonable selection of indication, single incision laparoscopic colorectal surgery is safe and feasible, and it also has a satisfactory cosmetic effect and better minimally invasive effect.

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  • Changes of c-kit and scf mRNA and Protein in Guinea Pig Gallbladder Fed on High Cholesterol Diet

    Objective To discuss the changes of c-kit/scf mRNA and protein in guinea pig gallbladder fed on high cholesterol diet. Methods Twenty guinea pigs were divided into two equal groups of 10 each:the control group and lithogenic group. Normal diet and high cholesterol diet was given to each group respectively. The period of stone permeation was six weeks. RT-PCR and Western blot were used to determin the expressions of c-kit and scf mRNA and protein. Results RT-PCR results showed that the expressions of c-kit mRNA(t=6.985,P<0.01) and scf mRNA (t=6.028, P<0.01)decreased significantly in lithogenic group compared with the control group. Western blot results showed that the expressions of c-kit protein (t=10.256, P<0.01) and scf protein (t=9.586, P<0.01)decreased significantly in lithogenic group compared with the control group. Conclusions The expressions of c-kit/scf mRNA and protein decrease during the formation of cholesterol gallstones in guinea pigs fed on high cholesterol diet. Inhibition of c-kit/scf pathway may play a role in the formation of cholesterol gallstones.

    Release date:2016-09-08 10:36 Export PDF Favorites Scan
  • NHE3在胆固醇结石胆囊组织中的表达

    目的研究NHE3在正常和胆固醇结石患者胆囊黏膜上皮细胞中的表达情况,并探讨NHE3表达异常与结石形成的关系 方法利用Western blot以及免疫组织化学方法检测10例正常与16例胆固醇结石患者胆囊黏膜上皮细胞中NHE3蛋白和亚细胞定位的表达情况。 结果Western blot和免疫组织化学染色方法均显示,与正常胆囊组相比,结石组胆囊黏膜上皮细胞中NHE3表达增高(P<0.05),NHE3同时表达于胆囊黏膜上皮细胞的顶质膜和胞浆内。 结论NHE3表达异常可能与胆囊胆固醇结石发病相关,其发生机理有待进一步研究证实。

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  • Single-Incision Laparoscopic Versus Laparoscopy-Assisted Subtotal Gastrectomy for Benign Gastric Ulcer and Duodenal Ulcer: A Retrospectively Comparative Study

    ObjectiveTo compare clinical outcome between single-incision laparoscopic subtotal gastrectomy (SILSG) versus laparoscopy-assisted subtotal gastrectomy (LASG) in treatment of benign gastric ulcer and duodenal ulcer. MethodsClinical data of 37 patients with benign gastric ulcer or duodenal ulcer who underwent laparoscopic subtotal gastrectomy between Jan. 2008 and Feb. 2015 at Shengjing Hospital of China Medical University was collected retrospectively. Among them, 15 patients underwent SILSG and 22 patients underwent LASG. Demographic, intraoperative, and postoperative data was analyzed and compared between the 2 groups. ResultsThe operative time of SILSG group was significantly longer than that of LASG group (P < 0.050). However, the postoperative hospital stay was significantly shorter (P < 0.050), and the total patient scar assesment scale (PSAS) score was significantly lower (P < 0.050) in the SILSG group than those of LASG group. There was no significant difference between the 2 groups with respect to other variables (P > 0.050), such as conversion rate, intraoperative blood loss, postoperative exhaust time, incidence of complication, and visual analog scale score of pain. All patients received postoperative follow up, and the period ranged from 6 months to 25 months, with a median of 11 months. During the follow up period, no one suffered from incision hernia and recurrence of ulcer. ConclusionCompared with LASG, SILSG is a technically feasible procedure with better cosmesis and equivalent curability.

    Release date:2016-10-21 08:55 Export PDF Favorites Scan
  • Curcumin can inhibit the lipopolysaccharide-induced up-regulation of endogenousβ-glucoronidase expression

    ObjectiveTo investigate the effect of curcumin on the expression regulation of endogenousβ-glucoronidase (β-GD) induced by lipopolysaccharide (LPS).Methods① Human normal intrahepatic biliary epithelial cell line (HiBEpiC) cells in the logarithmic growth phase were divided into blank control group (0 h group) and 7 different stimulation time groups. The cell density was adjusted to 1×104/mL, and the cells were stimulated with 100 mg/mL LPS for 1, 3, 6, 18, and 24 hours respectively, including another two groups where the cells were cultured with LPS-free medium for 18 and 24 hours after LPS stimulation for 24 h. ② HiBEpiC cells in the logarithmic growth phase were divided into blank control group, LPS+low, medium, and high concentration curcumin group. The cell density was adjusted to 1×104/mL. In the blank control group, cells were not stimulated with any reagent; in the LPS group, cells were stimulated with 100 mg/mL LPS, in the other three groups, the cells were stimulated with 100 mg/mL LPS and simultaneously 20, 40, and 80 μmol/L curcumin, respectively, for 24 hours. The expressions of c-myc and endogenous β-GD were detected by Western blot method.Results① The expressions of endogenous β-GD and c-myc in HiBEpiC cells gradually increased with the prolongation of treatment time by LPS, and the expression levels of β-GD and c-myc at each time point group were significantly different from those in the 0 h group (P<0.05). ② There were significant difference between any two groups of the blank control group, LPS group, LPS+low concentration of curcumin group, LPS+medium concentration of curcumin group, and LPS+high concentration of curcumin group (P<0.05).ConclusionCurcumin is able to inhibit the increased expression of endogenous β-GD induced by LPS, possibly via inhibiting expression of c-myc.

    Release date:2019-08-12 04:33 Export PDF Favorites Scan
  • 单孔腹腔镜胆囊逆行切除术的学习曲线分析

    目的 总结采用常规腹腔镜器械进行逆行切除法在单孔腹腔镜胆囊切除术中应用的学习曲线。 方法 回顾性分析中国医科大学附属盛京医院单一主刀医生于 2012 年 7 月至 2015 年 12 月期间施行的连续单孔腹腔镜手术的 120 例患者的临床资料,单孔腹腔镜胆囊切除术中采用常规腹腔镜器械进行胆囊逆行切除。 结果 所有患者的手术都顺利进行。手术施行期间,手术时间随手术例数增加而明显减少,10 例以后手术时间基本稳定。前期组和后期组患者的手术花费、帕瑞昔布钠剂量和美容评分比较差异均无统计学意义(P>0.05),但与前期组比较,后期组的手术时间较短,术中出血量较少,术后住院时间较短,中转多孔率和并发症发生率较低,视觉模拟评分(visual analogue scale,VAS)较低,差异均有统计学意义(P<0.05)。 结论 单孔腹腔镜胆囊切除术中采用常规腹腔镜器械进行胆囊逆行切除的学习曲线短,易于术者掌握。

    Release date:2017-06-19 11:08 Export PDF Favorites Scan
  • Application of Conventional Laparoscopic Instruments in Transumbilical Single Incision Laparoscopic Surgery in Colorectal Diseases

    目的 探讨利用常规腹腔镜器械完成经脐单孔腹腔镜结直肠手术的可能性和技术要点。方法 收集中国医科大学附属盛京医院微创外科于2009年4月至2010年1月期间施行的12例经脐单孔腹腔镜结直肠手术的临床资料。阑尾炎8例,均为女性,平均年龄40岁; 回盲部肿物2例,均为女性,其中1例为回盲部淋巴水瘤(68岁),另1例为回盲部溃疡性结肠炎(47岁); 乙状结肠息肉1例,女,55岁; 直肠癌1例,男,52岁。 12例均于脐部行2.5~3.0 cm长单切口,利用常规腹腔镜手术器械完成手术。结果 8例阑尾手术,手术时间20~50 min,出血量均少于10 ml; 2例回盲部切除术手术时间分别为60 min和90 min,出血量分别为10 ml和20 ml; 1例乙状结肠切除术用时120 min,术中出血约50 ml,术后4 d拔除引流管; 直肠癌手术时间210 min,术中出血少于200 ml,术后1周拔除引流管并出院。结论 利用常规腹腔镜手术器械完成经脐单孔腹腔镜结直肠手术安全可行。

    Release date:2016-09-08 10:49 Export PDF Favorites Scan
  • Experience about Transumbilical Single-Port Laparoscopic Appendectomy by Using Traditional Instrument

    目的 总结经脐单切口腹腔镜阑尾切除术的技术要点。方法 回顾性分析2012年1月至12月期间中国医科大学附属第四医院收治并行经脐单切口腹腔镜阑尾切除术的55例阑尾炎患者的临床资料。结果 54例患者手术均获成功,1例患者因腹膜后阑尾而中转为3孔法。手术时间为(40.6±12.3) min (35~90min),住院时间为(4.5±1.3) d (3~6d),住院费用为(1.2±0.3)万元(0.8~1.5万元),术后均无并发症发生。术后49例患者获访,随访时间为1~6个月,平均4.8个月。术后患者均恢复良好,脐部瘢痕不明显,美容效果较满意。结论 采用通用器械行经脐单切口腹腔镜阑尾切除术安全、可行,美容效果较佳。

    Release date:2016-09-08 10:34 Export PDF Favorites Scan
  • Laparoscopic Operation of Gastric and Gastroesophageal Junction Disease (Report of 59 Cases)

    Objective To investigate the feasibility and safety of laparoscopic operation of gastric and gastroesophageal junction diseases. Methods Between May 2004 and June 2009, 59 patients with gastric and gastroesophageal diseases were treated laparoscopically. The operative methods and maneuvers were evaluated and perioperative interventions, complications and efficacy of patients were analyzed. Results All operations were successfully completed laparoscopically except for one patient with gastric cancer who required a conversion to open surgery. No short-term complications occurred in all cases. No port transplant metastasis occurred for the patients with gastric cancer after an average of 36 months (1-60 months) follow-up. One patient died of liver metastasis 12 months after operation. The 3-year survival rate was 93.3% (14/15). Conclusion Laparoscopic surgery of the gastric and gastroesophageal junction diseases is feasible and safe with minimal invasiveness, which is worth popularizing.

    Release date:2016-09-08 10:50 Export PDF Favorites Scan
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