【Abstract】Objective To investigate the role of early surgical intervention in the treatment of fulminant acute pancreatitis (FAP).Methods Eight cases of FAP admitted to our institution from September 2003 to December 2004 were reviewed retrospectively.Results Averagely 3 organs dysfunction was diagnosed on admission or in the course of treatment in this group. One patient treated non-operatively was dead. Of 7 cases with early surgical intervention, one was died of ACS and ARF, One female patient with 32 weeks pregnancy survived, but the fetus was dead before surgery. Five cases were complicated with intra-abscess, which were cured re-operatively. Conclusion Early operative intervention in the treatment of FAP could prevent MODS and improve the survival.
ObjectiveTo introduce the current status of clinical application, value and perspective of fiberoptic ductoscopy.MethodsThe related literatures on advances in clinical application of fiberoptic ductoscopy were reviewed.ResultsFiberoptic ductoscopy is now widely used in breast diseases, especially complicated with nipple discharge, and it has a higher accuracy rate than routine examinations. With ductoscopy, ductal lavage,location, biopsy and treatment can be carried out.ConclusionFiberoptic ductoscopy has a greater value in diagnosis and treatment, we believe it will be better applied and further developed.
【Abstract】Objective To introduce the current research status, value and development future of Arg-Gly-Asp (RGD) peptides in diagnosis and treatment of neoplasms. Methods The current literatures on advances about RGD peptides in diagnosis and treatment of neoplasms were reviewed. Results RGD peptides, specificly recognizing and combining with integrin receptors, exist in extracellular matrix (ECM) of many kinds of organisms. After combining with integrin receptors, extrinsic RGD peptides can prevent tumor cells from adhering to ECM and migrating as the competitive inhibitor of intrinsic RGD peptides, suppress agiogenesis and induce tumor cells apoptosis, showing potential value of tumor specific imaging by targetal labelling neoplasms and treating tumors combining with other methods.Conclusion RGD peptides may be a new drug for diagnosis and treatment of neoplasms.
ObjectiveTo study the detection methods of micrometastasis in sentinel lymph nodes (SLN) and their clinical significance. MethodsFifty women with breast carcinoma were included. SLN in fifty breast carcinoma was identified by using methylene blue staining to detect and remove them for routine hematoxylin and eosin stain and histological exam. All negative SLNs were examined by serial section (SS) with the section interval of 250 μm and HE stain for microscopic examination and immunohistochemical (IHC) exam was performed with CK19 monoclonal antibody. Then the above three detection methods were analyzed. All patients had axillary lymph node dissection (ALND)，and all none sentinel lymph nodes (NSLN) were examined by Hamp;E staining.ResultsThe SLNs were identified in 45 of 50 patients with a detection rate of 90%. Sixteen SLNs were found positive with routine histological exam, the positive detecting rate was 35.56%, while the other 29 negative SLNs were found 7 and 6 cases of micrometastasis using SS and IHC methods，therefore the positive detecting rate was increased by 15.55% and 13.33%, respectively.Conclusion SS and IHC methods could detect the micrometastasis in negative SLN with routinely histological exam, increasing the positive detecting rate and decreasing the false negative rate.
Objective To summarize the advancement of breast cancer stem cells and genotyping and analyze the correlation between the two. Methods Relevant literatures about breast cancer stem cells and genotyping, which were published recently were collected and reviewed. Results Cancer stem cell origin theory was supported by researches of correlation between breast cancer stem cells and genotyping, which also explained the complexity of intrinsic subtypes and heterogeneity of breast cancer. Conclusions A new way can be detected to study the formation mechanism and biological characteristics of breast cancer at the cellular and molecular level by researches of correlation between breast cancer stem cells and genotyping, which are expected to provide new strategies and tools for diagnosis and treatment of breast cancer.
Objective To summarize the progress of clinical research on triple-negative breast cancer (TNBC). Methods Domestic and international publications on the study of TNBC in recent years were collected and reviewed. Results The patients with TNBC were younger, and their prognosis was poorer. Besides operation, chemotherapy was the major therapeutic tool for them. Currently the targeted therapy for epidermal growth factor receptor and its signal conducting system was applied to clinical therapy gradually， and it might benefit the patients with TNBC. Conclusion The study on TNBC may bring a new way for therapy.
【Abstract】Objective To give a summary of the current researches on hematopoietic stem cell niche. Methods Through extensive reviewing the related domestic and abroad literatures, the present summary reviews the components of hematopoietic stem cell niche, related cell factors and related cell signaling pathway participating in regulation of hematopoietic stem cell. Results The activities of hematopoietic stem cells are regulated by the niche (microenvironment), which is composed of hematopoietic stem cell and its surrounding cells. The regulation cannot be completed through one signaling pathway. Also, the self-renewal and differentiation of hematopoietic stem cell cannot be completed only through osteoblastic cells. Conclusion The niche regulates hematopoietic stem cells by different ways. With study in-depth, we will comprehensively understand the nature of stem cells and the study will provide a broader space to stem cell-based therapy.
Objective To summarize the status of tumor stem cells study in solid tumors. Methods Domestic and international publications on the study of tumor stem cells in solid tumors in recent years were collected and reviewed. Results Tumor stem cells exactly existed in solid tumors and played a very important role in tumorgenesis. Conclusion Tumor stem cells bring a new broad of perspective on solid tumor study.
目的 探讨捆绑式胰肠吻合术的改良方法。方法 对8例胰十二指肠切除术患者行捆绑式胰肠吻合术时采用带血管蒂的大网膜包绕空肠浆肌鞘，以达捆绑之目的。8例中行桥袢空肠造瘘5例，胆总管造口T管引流3例，并观察其术后5 d内每天平均引流液量。结果 8例患者均治愈出院，无胰瘘、胆瘘发生； 5 d内每天平均引流液量除第1天胆总管造口低于空肠造瘘外，其余4 d每天平均引流液量均明显高于空肠造瘘； 1例空肠造瘘者术后第8天继发胰腺残端出血，经电灼后止血。结论 带血管蒂的大网膜包绕空肠浆肌鞘完全可以代替粗线环绕空肠结扎，并使整个空肠浆肌鞘与胰腺紧贴，更利于胰肠间愈合； 空肠造瘘或胆总管造口对桥袢空肠均有减压引流的作用，但T管引流更简单、有效、省时。