ObjectiveTo explore the method for establishing a pig left lung orthotopic transplantation model. MethodsDetailed surgical procedures, including animal anesthesia, tracheal intubation, donor lung retrieval, and recipient transplantation, were thoroughly reported. By examining the histological morphology and blood gas analysis of the transplanted lung 2 hours after reperfusion, the histological changes and function of the transplanted lung were assessed. ResultsThis method was applied to four male Yorkshire pigs with an average weight of (40 ±2.50) kg for left lung in situ transplantation, effectively simulating conditions relevant to human lung transplantation. Two hours post-transplantation, arterial blood gas analysis showed PaO2 values ranging from 155.4 to 178.6 mmHg, PaCO2 values ranging from 53.1 to 62.4 mmHg, and PaO2/FiO2 ratios ranging from 310.8 to 357.2 mmHg. Hematoxylin and eosin (H&E) staining indicated a low degree of pulmonary edema and minimal cellular infiltration. ConclusionsThe pig left lung orthotopic transplantation model possesses strong operability and stability. Researchers can replicate this model according to the described methods and further conduct basic research and explore clinical translational applications.