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find Author "廖秀清" 3 results
  • 联合检测微卫星不稳定性和p16甲基化在早期肺癌中的诊断价值

    目的 研究联合检测微卫星不稳定性( MSI) 和p16 基因启动子甲基化在早期肺癌中的诊断价值。方法 对肺癌、癌前病变和正常肺组织采用PCR 单链长度分析法检测MSI, 采用甲基化特异PCR 法检测p16 甲基化。结果 癌前病变组MSI 的发生率显著高于肺癌组( P lt;0. 05) 和正常肺组织组( P lt;0. 01) , 肺癌组MSI 的发生率显著高于正常肺组织组( P lt;0. 01) ; 肺癌组p16 甲基化的发生率显著高于癌前病变组( P lt;0. 01) 和正常肺组织组( P lt;0. 01) , 癌前病变组p16 甲基化的发生率显著高于正常肺组织组( P lt;0. 01) ; 联合检测MSI 和p16 甲基化的敏感性显著高于单一检测MSI( P lt;0. 01) 和p16 甲基化( P lt;0. 05) ; 联合检测法与单一检测MSI 和单一检测p16 甲基化的特异性比较,差异无显著性意义( P gt;0. 05) 。结论 联合检测MSI 和p16 甲基化可以显著提高早期肺癌诊断的敏感性同时不降低其特异性, 值得临床推广。

    Release date:2016-08-30 11:52 Export PDF Favorites Scan
  • 慢性阻塞性肺疾病患者血浆网膜素-1 水平与病情严重程度的相关性研究

    目的研究血浆网膜素-1 在慢性阻塞性肺疾病(简称慢阻肺)患者临床评价中的价值,并进一步探讨其与慢阻肺急性加重病情严重程度的相关性。方法选取 124 例慢阻肺患者,按照病程分为急性加重组(64 例)与稳定期组(60 例),另选取 60 例无慢阻肺的健康成人作为对照组。急性加重组按照病情严重程度进行临床分级。检测研究对象的血浆网膜素-1 水平;分析慢阻肺患者血浆网膜素-1 水平与第 1 秒用力呼气容积占预计值百分比(FEV1%pred)、用力肺活量占预计值百分比(FVC%pred)、GOLD 分级、改良呼吸困难指数(mMRC)评分、急性加重风险评估分组、氧合指数(PaO2/FiO2)、动脉血二氧化碳分压(PaCO2)、慢阻肺急性加重临床分级等指标的相关性。结果慢阻肺患者的血浆网膜素-1 水平明显低于对照组(P<0.05),其中慢阻肺急性加重组的血浆网膜素-1 水平较稳定期组更低(P<0.01)。慢阻肺患者的血浆网膜素-1 水平与 FEV1%pred、FVC%pred 呈正相关,与 GOLD 分级、mMRC 评分、CAT 评分呈负相关(P<0.01)。慢阻肺急性加重患者血浆网膜素-1 水平与 PaO2/FiO2 呈正相关,与 PaCO2、呼吸衰竭类型、中性粒细胞百分比、住院天数、机械通气时间呈显著负相关(P<0.05)。随着 GOLD 分级的增加,慢阻肺急性加重患者血浆网膜素-1 水平进行性降低,在 ABCD 综合评估中临床症状越重者其血浆网膜素-1 水平越低。慢阻肺急性加重患者的血浆网膜素-1 水平与临床分级与呈显著负相关(P<0.01)。血浆网膜素-1 水平在慢阻肺急性加重不同临床分级之间有显著差异(P<0.05)。与入院第 1 天相比,慢阻肺急性加重患者出院前 1 天的血浆网膜素-1 水平明显增高(P<0.01)。结论血浆网膜素-1 与慢阻肺临床评价指标具有一定相关性,可作为评估急性加重的生物学标志物。

    Release date:2020-09-27 06:38 Export PDF Favorites Scan
  • Investigation of Cognition Degree and Clinical Use of 2011 GOLD New COPD Classification System in Respiratory Specialist from Chongqing

    Objective To investigate the cognition degree and clinical use of new COPD classification system of 2011 GOLD in respiratory specialists, and further analyze the reasons of failing to clinical use. Methods Respiratory specialists from 42 hospitals in Chongqing were investigated through questionnaire survey. The questionnaire contains two parts. The first part contains nine questions about the knowledge of 2011 GOLD new COPD classification system and its clinical use. The second part contains six questions about the reasons of failing to clinical use of the COPD classification system. Results A total of 204 valid questionnaires were recovered. More than 90% respiratory specialists had understood the new COPD classification system with different degree, and believed it is suitable for clinical use. More than twothirds respiratory specialists knew well the ways about CAT and mMRC, but only 24% specialists were using these ways. The main reasons of failing to clinical use were as follows: 60% specialists believed the pulmonary function test can evaluate the COPD classification, and 66. 7% specialists were limited by short visit time. The cognition degree and clinical use of the new COPD classification systemin the specialists from third grade A class hospitals was better than those from the other hospitals. But the difference was not significant among specialists with different professional title.Conclusion Respiratory specialists in Chongqing knew well about the new COPD classification systemin 2011 GOLD, but did not use it widely in clinical works due to the complicated operation of the new COPD classification system.

    Release date:2016-09-13 03:53 Export PDF Favorites Scan
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