Objective To decrease the operative difficulty, with the purpose of looking for an orthotopic liver autotransplantation model which not only materializes the liver transplantation but also possesses higher survival rate. Methods This model was established via portal vein perfusion in thirty rats, and from which the result of the liver after perfusion, the operative time and the survival rate were observed. Liver tissues were researched at 24 h after operation under the light microscope. Results This model was easy to be perfused, the operative time was (48±3.0) min and the survival rate was 96.7% (29/30). The structure of hepatic tissue was basically normal with a little hydropic degeneration under the light microscope. Few erythrocytes residual occurred in the interlobular arteries under the light microscope. Conclusion The orthotopic liver autotransplantation model via portal vein perfusion has an exclusively blockage pattern which possesses a higher survival rate. It prevents the injury of immunological rejection and purely reflects the hepatic ischemia-reperfusion. But it is better to be applied in the non-hepatic artery anastomosis or the research nothing to do with the hepatic artery because the hepatic artery does not have sufficient perfusion.
目的探讨间断低氧预适应对大鼠肝大部切除术后残余肝脏合并缺血再灌注引发过氧化损伤的保护作用。 方法78只SD大鼠，用SPSS软件将其随机分为4组：假手术组（SO组，n=6）、肝切除组（PH组，n=24）、肝切除合并缺血再灌注损伤组（IR组，n=24）和间断低氧预适应组（IHP组，n=24）。以无创伤血管夹阻断IR组大鼠入肝血流后切除肝脏的左叶和中叶（约占全肝的70%），20 min后开放入肝血流，残余肝脏发生了缺血再灌注损伤。将IHP组大鼠暴露于10%的低氧环境中，每日持续1 h，连续进行1周，最后1次低氧暴露后行肝切除术（同IR组）。SO组大鼠在术后2 h取材检测，其余各组分别于术后2、6、12及24 h进行检测。检测血清转氨酶（ALT、AST）水平和肝匀浆组织中超氧化物歧化酶（SOD）活性及丙二醛（MDA）含量。 结果术后2 h，PH组、IR组和IHP组大鼠血清ALT和AST水平均高于SO组（P<0.05）。在术后6、12和24 h，IHP组大鼠血清ALT和AST均高于PH组，但低于IR组（均P<0.05）。与IR组相比，IHP组大鼠术后各时间点残余肝脏中SOD活性明显升高，而MDA含量则显著降低（均P<0.05）。 结论间断低氧预适应对残余肝脏缺血再灌注损伤具有保护作用，其机理可能与提高肝脏的抗氧化能力有关。
Objective To investigate the protection on the intrahepatic cholangiocyte mediated by hypoxic preconditioning (HP) after liver transplantation and the role of vascular endothelial growth factor (VEGF). Methods The model of autologous liver transplantation was established, and the rats were divided into 3 groups: autologous liver transplantation group, hypoxic preconditioning before operation group (HP group) and sham operation group. At 6, 12, 24, 48 h after operation, blood samples were collected for examination of the serum total bilirubin (TBIL), direct bilirubin (DBIL) and alkaline phosphatase (ALP), and the expression of VEGF was detected by immunohistochemical method. The pathological changes of cholangiocytes were observed by light microscope. Results As compared with autologous liver transplantation group, the levels of seurm TBIL, DBIL and ALP in HP group were lower (P＜0.05), while the expression of VEGF in HP group was higher at the whole process (P＜0.05). The degrees of billiary epithelium damage and inflammatory infiltration in autologous liver transplantation group were more severe than those in HP group. Conclusion HP has protective effect on cholangiocytes after liver transplantation, in which VEGF may play an important role.