ObjectiveTo observe the protective effect of tanshinone Ⅱ A on the mouse liver ischemia-reperfusion injury (IRI) model and preliminarily explore its mechanism of alleviating liver injury.MethodsThe IRI mouse model was established after the pre-treating with tanshinone Ⅱ A. Then, the serum and liver tissue of mice were collected to detect the changes of liver function, histopathology, liver cell apoptosis, and inflammatory factors. In addition, the protein expression levels of high mobility group box 1 (HMGB1), advanced glycosylation end-product specific receptor (RAGE), and Toll like receptor 4 (TLR4) in the liver tissues were detected by the Western blot method.ResultsAll data were analyzed by the homogeneity of variance test. The results of factorial design showed that the levels of ALT and AST in the serum, the pathological score and apoptosis index, the inflammatory response, as well as the expressions of HMGB1, TLR4 and RAGE proteins in the liver tissues were decreased significantly (P<0.05) in the sham operatation plus tanshinone Ⅱ A mice, which were increased significantly (P<0.05) in the IRI mice, which were antagonized synergistically by the tanshinone ⅡA and IRI (P<0.05).ConclusionsTanshinone ⅡA could reduce the liver IRI and inflammatory response in mouse. These effects might be related to the down-regulations of TLR4, HMGB1, and RAGE expressions.
目的比较全层连续缝合法和分层缝合法在急诊腹部手术中的切口愈合效果。 方法将符合纳入标准的急诊腹部手术患者随机分为全层缝合组和分层缝合组，比较2组患者的切口愈合效果。 结果全层缝合组的关腹时间短于分层缝合组（P=0.000），但在切口液化发生率、切口感染发生率、总切口并发症发生率、切口愈合及术后住院时间方面，2组的差异均无统计学意义（P＞0.05）。26例细菌培养结果为阳性（全层缝合组10例，分层缝合组16例）的患者中，致病菌主要为大肠埃希菌（11例）和铜绿假单胞菌（7例）。 结论相较于分层缝合法，急诊腹部手术中采用全层缝合法既不增加切口并发症的发生率，又节省手术时间，对于危重患者具有重要的意义。