Objective To establish interstitial cells of Cajal （ICC） loss models caused by incomplete small intestinal obstruction in rats with modified method and verify it. Methods Modified method was used to establish the models， making the ring around the small intestine but not through it. Morphological changes were observed by general signs， pathological changes were tested by HE staining and transmission electron microscope （TEM）， and changes of ICC number were tested by immunohistochemistry staining. Results Success rate of this method was 56% （28/50）， weight loss happened compared with before operation in ileus group （P＜0.01）. Hyperemia and swelling were observed in ileus group， and gastric retention was obvious. Results of HE staining and TEM showed that there was obvious inflammatory change， and ICC reduced was observed by immunohistochemistry. Conclusion ICC loss models caused by incomplete small intestinal obstruction meet the basic performance， and can be used for further research.
目的 初步观察电针足三里穴对不全肠梗阻大鼠小肠Cajal间质细胞（interstitial cells of Cajal，ICC）数量变化的影响，为进一步探讨电针足三里穴对ICC表型变化的影响奠定基础。方法 采用圈套法造成不全肠梗阻从而建立ICC数目减少的SD大鼠模型。取20只雌性大鼠采用简单随机法均分成正常对照组、不全肠梗阻30d未电针足三里穴组（梗阻组）、不全肠梗组30d电针足三里穴组（足三里组）和不全肠梗阻30d电针阴陵泉穴组（阴陵泉组） 4组。其中足三里组和阴陵泉组分别连续电针足三里穴或阴陵泉穴7d后，取小肠组织采用免疫组化方法以及免疫荧光观察ICC数量的变化。结果 正常对照组、足三里组、梗阻组及阴陵泉组ICC阳性面积分别为（102 051.00±16 969.38） μm2、（92 642.12±14 854.49） μm2、（45 221.33±6 230.20） μm2和（63 136.16±8 863.91） μm2，各组间差异有统计学意义（F=21.240，P＜0.001），其中足三里组的ICC阳性面积较梗阻组高（P＜0.05）。结论 电针足三里穴可使不全肠梗阻大鼠小肠受损的ICC数量得到部分恢复，但其具体机理有待进一步研究。