Objective To refine the loss of heterozygosity (LOH) on chromosome 7q21－22 and identify the new tumor suppressor gene(s) in colorectal tumorigenesis. Methods Fifteen polymorphic microsatellite markers were analyzed on chromosome 7 and another 5 markers were applied on chromosome 7q21-22 region in 83 cases of colorectal cancer and normal DNA by PCR. PCR products were electrophoresed on an ABI Prism 377 DNA sequencer. GeneScan 3.1 and Genotyper 2.1 software were used for LOH scanning and analysis. Results A distinct region of frequent allelic deletion was observed on chromosome, another 5 polymorphic microsatellite markers were applied to 7q21-22 and the minimal region of frequent LOH was established on 7q21-22 spanning the D7S657, D7S646 locus. Conclusion Through detailed deletion mapping studies, a critical and precise region spanning the D7S657, D7S646 locus is identified, which must contain one or more unknown tumor suppressor gene(s) on colorectal cancer.
Objective To investigate the effect of ABO-incompatible liver transplantation on patients with acute hepatic failure. Methods A retrospective review was undertaken on the clinical data of 3 cases （1 case of Budd-Chiari syndrome, 1 case of liver cancer and 1 case of severe hepatitis B were included） undergoing ABO-incompatible liver transplantation for acute hepatic failure. The patients were given quadruple immunosuppression after operation. Results Postoperative complications including pulmonary infection, central pontine myelionlysis and acute rejection was suffered from by the patient of Budd-Chiari syndrome who was then given a positive deal and had survived for more than 14 months. The patient of liver cancer was aggravated by severe infection who died of multiple organ failure on day 13 after operation. The patient with severe hepatitis B was made more severe by acute renal failure whose kidney function was restored with continuous renal replacement therapy. Conclusion The outcome of ABO-incompatible liver transplantation can be improved with refined peri-transplant management and better immunosuppressive strategies. ABO-incompatible liver transplantation should be viewed as an important option in patients with acute hepatic failure awaiting an emergency procedure.