Objective To investigate the surgical technique of establ ishing a rel iable rat model of orthotopic l ivertransplantation. Methods A total of 200 adult male SD rats weighing 200-250 g and 60 adult male Wistar rats weighing230-280 g were adopted. The weight of donor was 30 g less than that of receptor. Syngeneic group of SD-SD rats (SD-SD group, n=70) and allogeneic group of SD-Wistar rats (SD-Wistar group, n=60) l iver transplantation were performed, respectively. Orthotopic l iver transplantations in rats were performed using modified Kamada’s two-cuff technique. Under the sufficient exposure of the porta hepatis, the l iver was perfused through the cold of perfusion of portal vein without touching the l iver. The anastomosis of the suprahepatic vena cave was sutured end- to-end with 8-0 prolene l ine. Guided by double l ine, the continuity of portal vein was establ ished by cuff method easily. The fluid was supplemented sufficiently after operation to maintain the stabil ization of hemodynamics. Results The time for donor operation and receptor operation was (38.2 ± 2.5) minutes and (45.6 ± 3.5) minutes, and anhepatic time was (15.1 ± 2.2) minutes.The successful rate was 93%. The survival rate after 1 week was 92%. There was a significant difference when compared with traditional method (P lt; 0.05). There were 64 survivals in SDSD group and 57 in SD-Wistar group after l iver transplantation, and the survival time was 2-9 months (mean 145 days) and 8-20 days (mean 10.5 days) respectively. The l iver function recovered well in SD-SD group, while in SD-Wistar group the l iver functional failure and acute rejection occurred in pathology 3-5 days after l iver transplantation, all of which ended with death without any therapy. Conclusion The modified method is proved to be ideal for its advantages of simple operation, short anhepatic phase and high operative successful rate.
目的:探讨低能量CO2激光切除扁桃体切除术中手术技巧与并发症的关系,以改进手术技巧,减少手术并发症。方法:对我科51例低能量CO2激光扁桃体切除术的患者进行回顾性研究分析。观察、记录扁桃体切除所需手术时间、术中出血量、术后疼痛时间及程度、术后创面反应程度、术后再出血、术后术区瘢痕共6项指标。结果:低能量CO2激光扁桃体切除术主要的并发症是术后较轻的伤口疼痛、术中少量出血,无术后再出血及术后术区瘢痕。结论:低能量CO2激光扁桃体切除术,出血量少,术后疼痛小,反应轻,手术方法易掌握,提高手术技巧可进一步减少低能量CO2激光扁桃体切除术并发症,更好体现低能量CO2激光扁桃体切除术是扁桃体切除术中一种安全、有效、微创的术式。
Objective To review the complex situation，surgical experience，and surgical technique of laparoscopic cholecystectomy （LC）．Methods The clinical data of 86 patients who underwent LC in our hospital and the consulting hospitals from February 2005 to April 2011 were retrospectively analyzed．According to the complicated conditions，the different surgical techniques were applied during LC such as lamellar dissection，aspirater stripping method，laparoscopic suture，and intraoperative cholangiography．Results The operation time was （68±23）min （25－210 min），blood loss was （55±13）ml （15－200 ml）．LC was successfully performed in 84 of 86 cases．Only two patients received transferring laparotomy because of scar tissue in Calot triangle that was hard to dissect．No severe complications occurred，such as biliary leakage，bleeding after operation，stricture of bile duct，retained calculus，and so on．All the patients were cured before discharge．Conclusion Lamellar dissection，aspirater stripping method，and laparoscopic suture combined with the view of laparotomy can be used to deal with complicated LC successfully．
【Abstract】ObjectiveTo discuss the technique for pancreas transplantation alone(PTA). MethodsEighty-eight SD rats were used as donors and recipients. The PTA was performed with enteric drainage（E-D group, n=22） or bladder drainage（B-D group, n=22）. The donor’s abdominal aorta(splenic artery) and portal vein(splenic vein) were anastomozed with the recipient’s abdominal aorta(end-to-side) and left renal vein(end-to-end). Blood glucose, food intake, water intake and urine volume were recorded before transplantation and on the 1st day, 3rd day, 7th day, 14th day and 30th day after transplantation. Results The mean PTA time was (33.1±11.1) min (donors) and (51.7±14.7) min(recipients). The grafts experienced no warm ischemia and the mean cold ischemia time was (46.5±17.1) min. After the successful PTA, the recipients’ blood glucose decreased on the first day after transplantation and reached normal on the third day. Their food intake, water intake and urine volume decreased and became stable 14 days later. ConclusionSuccessful PTA can restore the pancreatic endocrine function in diabetic rats. It is very important to master the technique for the operation.
ObjectiveTo evaluate the effectiveness of mitral valve repair for mitral regurgitation. MethodsWe retrospectively analyzed the clinical data of 47 patients underwent mitral repair in General Hospital of Ningxia Medical University between January 2010 and June 2014 year. There were 36 males and 11 females with age of 10 months to 65 years, mean age of 42.38±15.27 years. ResultsThere was no operative death within follow-up time of 18±7 months (ranged 14 to 1 586 days). Mitral valve function was normal or traces regurgitation in 33 patients (70.21%). Mild mitral regurgitation occurred in 11 patients (23.40%). Postoperative transesophageal echocardiography showed that 2 patients (4.26%) had moderate regurgitation. They underwent mitral valve repair again and cured. One patient (2.13%) underwent mitral valve replacement because of moderate to severe regurgitation. The dimensions of left atrium and left ventricle obviously decreased and heart function improved significantly compared with preoperative ones. ConclusionStrict control of surgical indications for different valve disease, the use of mitral valve repair technique, mitral surgery can get a good clinical efficacy. Preoperative diagnosis by transesophageal echocardiography, intraoperative monitoring, and immediate postoperative assessment for mitral valve repair results provide good technical support.
ObjectiveTo evaluate the application of modified perineal stapled prolapse resection in treatment of external rectal prolapse, and to assess the preliminary efficacy. MethodsClinical data of 11 patients with external rectal prolapse underwent modified perineal stapled prolapse resection in The First Affiliated Hospital of Guangzhou University of Chinese Medicine between December 2013 and August 2015 were collected for retrospectively analysis. ResultsAll patients with external rectal prolapse were performed modified perineal stapled prolapse resection with general anesthesia and (or) epidural anesthesia. The average operation time was (53.6±6.1) min (40-85 min) and the average blood loss was (35.3±10.1) mL (20-60 mL). The time of the first flatus was less than 24 h after operation, the time of the first defecation was (44.6±3.3) h (40-52 h) after operation. The average hospital stay was (9.7±1.8) d (7-12 d) and average cost was (34 635.8±1 268.8) RMB (31 796-36 212 RMB). None of them occurred serious complications such as uncontrollable anastomotic stoma bleeding, anastomotic leakage, pelvic abscess, bowel obstruction, and anastomotic straitness. All of the 11 patients were followed up for (4.7±0.8) months (3-7 months), and all patients did not suffered from recurrence and fecal incontinence during follow-up period. ConclusionsThe essential procedure for modified perineal stapled prolapse resection is cutting the prolapse open at 3 and 9 clock directly at the same time by linear staplers, and dissecting mesorectum before using the contour satpler. Modified perineal stapled prolapse resection is a safe and effective operation technique for the external rectal prolapse.
Objective To investigate the benefits of using a stapler tractor in the treatment of segmental bronchus during lung segmentectomy through detailed video replay analysis of surgical procedures. Methods We collected data from patients who underwent segmentectomy performed by the same surgical team in the Department of Thoracic Surgery, the First Affiliated Hospital of Nanjing Medical University, from November 2020 to August 2023. After excluding data that lacked analysis parameters, the remaining patients were divided into four groups based on the methods used for dissociating segmental bronchus: a stapler tractor group (group A), a stapler with bronchial stretching group (group B), a stapler only group (group C), and a silk ligature group (group D). Then, we compared baseline data and videotaped surgical details across all groups. Surgical details included the success rate of one-time segmental bronchus dissociation and severance, the time taken for successful one-time dissociation and severance of the segmental bronchus, the incidence of bleeding during bronchus dissociation, the conversion rate to thoracotomy during surgery, and surgical outcomes such as total operative time, postoperative hospitalization days, postoperative thoracic drainage volume, and pulmonary air leakage rate. Results The study included 325 patients (203 in the group A, 62 in the group B, 29 in the group C, and 31 in the group D). There was no statistically significant difference in baseline data among the four groups. However, significant differences were found in terms of total operation time, postoperative hospitalization days, intraoperative blood loss, segmental bronchial stump length, postoperative air leakage rate, hemorrhage rate during segmental bronchial dissociation, and conversion to thoracotomy rate among the four groups (P<0.05). ConclusionUsing a stapler tractor for dissociating segmental bronchus in lung segmentectomy results in shorter operative time, less risk of intraoperative bleeding, and less surgical complications. This study provides valuable evaluation methodologies through the analysis of video replay surgical details, contributing to the improvement of lung segmentectomy quality.