【摘要】 目的 探讨甘油三酯（triglyceride，TG）和总胆固醇（total cholesterol，TC）试剂对血清总胆汁酸（total bile acid，TBA）检测结果的影响及解决办法。 方法 2008年1月-2009年10月采用魅力2000全自动生化分析仪，首先单独检测20份血清标本的TBA 含量。然后分别检测TC和TG后进行TBA含量检测。最后设定特殊检测程序和清洗程序后再按TC→TBA，TG→TBA顺序进行TBA含量检测。 结果 单独检测20份血清标本的TBA结果均值为7.2 μmol/L;按TC→TBA，TG→TBA顺序检测结果均值分别为13.5 μmol/L和14.3 μmol/L，单独和组合测量方法测定TBA结果有统计学意义（Plt;0.05）。设定特殊检测程序和清洗程序后按TC→TBA，TG→TBA顺序检测结果均值分别为7.4 μmol/L和7.5 μmol/L，与单独测量TBA结果相比，无统计学意义。 结论 TG和TC试剂对TBA检测产生干扰的原因是试剂成分中含有浓度较高的TBA，在魅力2000全自动生化分析仪上，设定特殊检测程序和清洗程序，能有效消除TG、TC试剂对TBA检测的影响。【Abstract】 Objective To explore the influence and procedure improvement strategies of triglyceride (TG) and cholesterol reagent on the result of serum total bile acid (TBA) test. Methods The study was carried out between January 2008 to October 2009. The charming 2000 automatic biochemical analyzer was used. First, TBA content of 20 serum samples was independently tested. Then TBA was detected after the detection of TG and total cholesterol (TC) concentration. Finally, TBA test was conducted with special testing procedures and cleaning procedures of TC→TBA and TG→TBA method. Results The average concentrate of TBA of 20 serum samples was 7.2 μmol/L, while the average concentrates of TBA tested by TC→TBA and TG→TBA methods were 13.5 μmol/L and 14.3 μmol/L, respectively. With special testing procedures and cleaning procedures of TC→TBA and TG→TBA tests, the average concentrates of TBA were 7.4 μmol/L and 7.5 μmol/L, respectively. Conclusion High concentration of TBA in triglyceride and cholesterol reagents contributes substantially to the interference in serum total bile acids detection. With the charming 2000 automatic biochemical analyzer, special testing procedures and cleaning procedures are able to effectively eliminate the interference of triglycerides and cholesterol reagents from TBA detection.
ObjectiveTo evaluate the complete blood count performance quality of Sysmex-XN automatic hematology analyzer. MethodsWe investigated the precision rate, residual contamination rate, analytic linearity range, and background counting of Sysmex-XN-B3 analyzer. ResultsThe inner and inter-group precision test showed that the inaccuracy of the analyzer was lower than the allowable standard of 1/4 (CLIA'88). The highest level of residual contamination rate was 0.12%, lower than the standard of manufacturer (≤1%). Linearity evaluation showed that the white blood cell count analytic linear range was from 0.51×109/L to 393.40×109/L, the red blood cell count analytic linear range was from 0.51×1012/L to 8.15×1012/L, the hemoglobin analytic linear range from 15.0 g/L to 244.5 g/L, and the platelet count analytic linear range was from 3.0×109/L to 2 072.5×109/L. Background counting was also lower than the standard of manufacturer. Comparison between the two different series of analyzers showed that the inaccuracy rate of Sysmex-XN-B3 was not only lower than the standard of National Center for Clinical Laboratories, but also lower than the standard of 1/2 (CLIA'88). ConclusionSysmex-XN automatic hematology analyzer has a high performance in capability evaluation. It is an excellent tool for routine hematologic blood examination.