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应用Spife Combo电泳仪进行血红蛋白电泳的质量保证及特殊故障分析

The Establishment and Application of Quality Control System on Sysmex-XN Series Automatic Blood Cell Analyzer

ObjectiveTo establish a comprehensive, appropriate quality control system on the Sysmex-XN series automatic blood cell analyzer to improve the reliability of blood cell count results. MethodsRoutine quality control was carried out every day, using high, normal and low levels of fresh whole blood controls offered by Sysmex. The results from the Sysmex online quality control was got timely. We monitored the variation of mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) with the moving average method real-timely, and quality control was performed for fresh whole blood samples of patients once every two hours. ResultsEverything was under control, and the moving average was within the permitted range. ConclusionThe reliability of laboratory blood cell count results can be effectively improved by using this quality control system effectively and comprehensively on the Sysmex-XN automatic blood cell analyzer.

不同厂商紫头真空采血管的血常规结果比对

目的：对不同厂商的紫头真空采血管进行评估，其血常规结果是否有差异。方法：同时使用美国BD公司和成都瑞琦公司生产的紫头真空采血管采集40份新鲜全血标本，分别在自动血液分析仪XE-2100进行检测，再进行白细胞(WBC)、红细胞(RBC)、血红蛋白(HGB)、血小板(PLT)等参数的相关性比较。结果：WBC、RBC、HGB和PLT等参数的差异百分率分别为：0.49%、0.05%、0.13%和1.01%；相关系数(r)分别是：0.9985、0.9964、0.9966和0.9932；t检验的P值分别是：0.9706、0.9020、0.9679、0.9061，均gt;0.05。结论：真空采血管普遍应用于临床标本的采集，由于真空度、抗凝剂的量、管壁的质量对血常规结果均有影响，不同厂商的真空采血管质量不完全相同，通过新鲜全血的结果比对，美国BD公司和成都瑞琦公司生产的紫头真空采血管的血常规结果相关性好，其相关系数均在0.99以上，P值均gt;0.05，无显著性差异，差异百分率小，均lt;±2.0%的可接受范围，表明可用于临床。