Objective To explore surgical technique and clinical outcome of transabdominal preperitoneal hernia repair (TAPP) and totally extraperitoneal hernia repair (TEP) in inguinal hernia repair. Methods 〗The clinical data of 23 patients underwent laparoscopic herniorrhaphy were retrospectively analyzed. Nine patients were underwent TAPP, and 14 patients underwent TEP. Results 〗None of patients was changed to open operation. Only one patient of TEP was changed to TAPP. Average operative time was (82.1±40.6) min. Blood loss was (5.7±3.0) ml. Disruption of peritoneum happened to 2 patients underwent TEP. The rate of postoperative complications was 21.7% (5/23), which included serum swelling (1 case), scrotal emphysema (2 cases), transient neurapraxia in the area of repair (1 case) and urinary retention (1 case). No chronic pain, foreign body sensation and infection were found. Average hospitalization after operation was (4.3±0.9) d. No recurrence was observed during a follow-up period of 1-15 months. Conclusion 〗Laparoscope herniorrhaphy is safe due to lower recurrence and complications. It also has the advantages of slight pain and rapid recovery.
目的:探讨人凋亡相关新基因PNAS-4(hPNAS-4)基因通过脂质体转染至Lewis肺癌LL2细胞后对放射治疗的增敏作用。〖HTH〗方法〖HTSS〗:用脂质体介导的转染技术,将hPNAS-4基因导入Lewis肺癌LL2细胞中,Western blot鉴定其过表达后,观察X射线照射对其集落形成的影响;流式细胞仪检测hPNAS-4基因或／和放疗(0,1,2,4,6 Gy)对LL2细胞生长抑制及凋亡的影响。〖HTH〗结果〖HTSS〗:通过Western blot证实了hPNAS-4基因在LL2细胞中的过表达。Lip-hPNAS-4加放射治疗处理组细胞的生存能力明显降低,肿瘤细胞的克隆形成明显减少,流式细胞术检测体外培养的肿瘤细胞凋亡明显增加。〖HTH〗结论〖HTSS〗:hPNAS-4基因联合放射治疗能产生协同抗肿瘤效应。
目的 探讨Ultrapro轻量型网片在腹腔镜腹股沟疝修补术中的优点和应用价值。方法 回顾性总结分析我院2008年8月至2010年10月期间，腹腔镜腹股沟疝修补术中应用Ultrapro轻量型网片的28例患者的临床资料。结果 28例患者共行疝修补术36例次，其中实施经腹腹膜前腹腔镜疝修补术（TAPP） 9例次，腹腔镜全腹膜外疝修补术（TEP）27例次；1例TEP中转行TAPP。平均手术时间为（103.4±39.8） min，术中平均出血量为（7.7±4.9） ml，术后平均住院时间（4.8±1.4） d。术后修补区暂时性神经异常1例，阴囊积液2例，尿潴留1例。28例均随访（15±1.6）个月（1～25个月），期间未观察到网片感染、疝复发、慢性疼痛、异物感或睾丸萎缩等并发症。结论 Ultrapro轻量型网片在腹腔镜腹股沟疝修补术中应用方便、视野清晰、柔软、硬度适中、放置定位容易，术后患者并发症少、恢复快，具有较好的临床应用价值。
Objective To explore the important role of preperitoneal space in laparoscopic transabdominal preperitoneal （TAPP） and totally extraperitoneal （TEP） hernia repair. Methods The clinical data of 66 patients （78 sides） performed laparoscopic TAPP and TEP hernia repair from January 2008 to April 2011 in this hospital were analyzed retrospectively. Results TAPP hernia repair were performed in 16 cases （20 sides），TEP hernia repair were performed in 50 cases （58 sides）. Three cases of TEP hernia repair transferred to TAPP hernia repair. The unilateral operation time was （86.92±36.38） min，intraoperative bleeding was （6.08±3.43） ml. Postoperative complication rate was 16.7% （11/66），including 3 cases of postoperative serum swelling，3 cases of temporary paraesthesia of nerve feeling in the repair area，2 cases of scrotum emphysema，2 cases of urinary retention，and 1 case of intestinal obstruction. There were 2 cases of recurrence. The hospital stay was （4.52±0.99） d. The return to activities and working time was （10.32±1.86） d after discharge. Sixty-six cases were followed up for （18.56±1.96） months （range 1-38 months），the patch infection，chronic pain，and testicular atrophy complications were not been observed. Conclusions Acquainting and mastering laparoscopic preperitoneal space and its important structure are the key to avoid intraoperative and postoperative complications of laparoscopic inguinal hernia repair.
Objective To explore the effect of NaOH on the surface morphology of three-dimensional (3D) printed poly-L-lactic acid (PLLA) mesh scaffolds. Methods The 3D printed PLLA mesh scaffolds were prepared by fused deposition molding technology, then the scaffold surfaces were etched with the NaOH solution. The concentrations of NaOH solution were 0.01, 0.1, 0.5, 1.0, and 3.0 mol/L, and the treatment time was 1, 3, 6, 9, and 12 hours, respectively. There were a total of 25 concentration and time combinations. After treatment, the microstructure, energy spectrum, roughness, hydrophilicity, compressive strength, as well as cell adhesion and proliferation of the scaffolds were observed. The untreated scaffolds were used as a normal control. Results 3D printed PLLA mesh scaffolds were successfully prepared by using fused deposition molding technology. After NaOH etching treatment, a rough or micro porous structure was constructed on the surface of the scaffold, and with the increase of NaOH concentration and treatment time, the size and density of the pores increased. The characterization of the scaffolds by energy dispersive spectroscopy showed that the crystal contains two elements, Na and O. The surface roughness of NaOH treated scaffolds significantly increased (P<0.05) and the contact angle significantly decreased (P<0.05) compared to untreated scaffolds. There was no significant difference in compressive strength between the untreated scaffolds and treated scaffolds under conditions of 0.1 mol/L/12 h and 1.0 mol/L/3 h (P>0.05), while the compression strength of the other treated scaffolds were significantly lower than that of the untreated scaffolds (P<0.05). After co-culturing the cells with the scaffold, NaOH treatment resulted in an increase in the number of cells on the surface of the scaffold and the spreading area of individual cells, and more synapses extending from adherent cells. Conclusion NaOH treatment is beneficial for increasing the surface hydrophilicity and cell adhesion of 3D printed PLLA mesh scaffolds.