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find Author "朱洪生" 18 results
  • 冠状动脉旁路移植术患者术后早期运动耐量改善的影响因素

    目的 探讨冠状动脉旁路移植术(CABG)患者术后早期运动耐量改善的影响因素,以提高手术疗效.方法随机选择30例行CABG患者,术前和术后1~3个月进行平板运动试验(TET),以手术前后运动功量的差值为应变量,各项临床指标与手术情况为自变量,进行Logistic多元回归分析. 结果 无手术死亡.术后运动功量等运动耐量指标及心肌缺血指标有明显改善(Plt;0.001),术前左心功能、心肌梗死史、心绞痛、高血压和乳内动脉(IMA)移植是影响手术疗效的主要因素. 结论 CABG能显著提高运动耐量,改善心肌缺血,了解并重视这些影响因素将有助于更好地选择手术病例,预测手术疗效.

    Release date:2016-08-30 06:32 Export PDF Favorites Scan
  • P-选凝素与心肌缺血-再灌注损伤

    研究心肌缺血-再灌注损伤中P-选凝素(P-selectin)的重要作用.P-selectin是一种糖蛋白黏附因子, 存在于内皮细胞和血小板,并介导血小板、内皮细胞和多形核白细胞(PMNs)等之间的相互作用,且与一氧化氮有着密切的关系,形成了许多复杂的炎症病理过程,在心肌缺血-再灌注损伤中起到了关键的作用.特别是P-selectin与晚期再灌注损伤、血小板及心肌损伤中治疗作用、最新的P-selectin基因缺陷小鼠和糖尿病小鼠等的心肌缺血-再灌注损伤中表现的深入研究,使其在缺血-再灌注损伤中的重要性和复杂性显得更加突出.

    Release date:2016-08-30 06:32 Export PDF Favorites Scan
  • 自身成体干细胞移植治疗心肌梗死

    心血管疾病,尤其是心肌梗死已成为人类死亡的主要疾病.自身成体干细胞移植为心肌梗死的治疗带来了新的希望.目前可供移植的种子细胞包括骨骼肌卫星细胞,骨髓干细胞,内皮祖细胞,自体移植避免了免疫排斥和伦理道德问题.近期实验表明移植的干细胞能在心肌疤痕中存活,增强心功能.当前需要进一步研究干细胞移植后与心肌微环境的联系,理想的移植细胞数目和移植技术以及对整个心功能可能产生的影响.

    Release date:2016-08-30 06:32 Export PDF Favorites Scan
  • 血管紧张素转换酶抑制剂对心肌再灌注损伤的作用

    心肌再灌注损伤可表现为再灌注性心律失常、心肌顿抑和心肌坏死.研究表明心脏局部的肾素血管紧张素系统和冠状动脉内皮细胞功能失常在心肌再灌注损伤中起重要作用.血管紧张素转换酶抑制剂能抑制转换酶活性,减少血管紧张素Ⅱ的形成,并能作用于激肽酶Ⅱ,抑制缓激肽的降解,后者可促进前列环素和一氧化氮(NO)的产生.近年来血管紧张素转换酶抑制剂被用于防护心肌再灌注损伤,在防护再灌注性心律失常、心肌顿抑和心肌坏死等方面显示了较好的治疗效果.

    Release date:2016-08-30 06:35 Export PDF Favorites Scan
  • MicroRNA-129 Promotes Cardiomyogenesis in Bone Marrow Mesenchymal Stem Cells

    Objective To explore the induction of cardiomyogenesis of microRNA-129 (mir-129) in rat bone marrowmesenchymal stem cells (BM-MSCs) and its mechanism. Methods BM-MSCs were isolated from Sprague-Dawley rats and cultured in vitro. Overexpression of mir-129 or both mir-129 and glycogen synthase kinase-3β (GSK-3β) in BM-MSCs was produced with a lentiviral vector system. All the BM-MSCs were divided into four groups: control group (MSCs),Lentiviral vectors+MSCs group (Lv-MSCs),mir-129 transfection group (mir-129-MSCs),and mir-129+GSK-3βdouble transfection group (mir-129+GSK-3β-MSCs). Five-Azacytidine (5-Aza) (10 μmol/L) was used to induce BM-MSCsdifferentiation into cardiomyocytes. On the 1st,5 th,10 th,15 th and 20 th day after induction,realtime-PCR was performedto detect mRNA levels of GATA-4,Nkx2.5 and MEF-2C. On the 10 th,15 th and 20 th day after induction,Western blottingwas performed to examine expression levels of cTnI,Desmin,GSK-3β,phosphorylated β-catenin and dephosphorylated β-catenin. Results Compared with the control group,at respective time points,mRNA levels of cardiomyogenic genes and expression levels of cardiomyocyte-related proteins of mir-129 transfection group were significantly elevated,theexpression level of GSK-3β was significantly decreased,and the ratio of dephosphorylated/phosphorylated β-catenin was significantly elevated. When both mir-129 and GSK-3β were overexpressed in BM-MSCs,mRNA levels of cardiomyogenicgenes and expression levels of cardiomyocyte-related proteins were significantly lower than those of mir-129 transfection group,and the ratio of dephosphorylated/phosphorylated β-catenin was significantly decreased. Conclusion Overexpression of mir-129 can promote cardiomyogenesis of rat BM-MSCs possibly via inhibiting GSK-3β production and thus decreasing the inhibition of phosphorylation of β-catenin which then enters the nucleus and activates downstream signaling pathways that regulate cardiomyogenic differentiation of BM-MSCs.

    Release date:2016-08-30 05:47 Export PDF Favorites Scan
  • Surgical Treatment of Partial Atrioventricular Canal Defect in 66 Cases

    Objective To summarize the experiences of surgical treatment for partial atrioventricular canal defect. Methods The data of 66 patients of surgical treatment for partial atrioventricular canal defect from January 1984 to December 2007 were analyzed retrospectively. The cleft of mitral valve presented in all of those patients. There were 52 cases with direct suture on cleft, 8 cases with direct suture with commissurroplasty, 1 case with posterior leaflet plasty, 3 cases with direct suture St.Jude ring and 2 cases mitral valve replacement. The ostium primum atrial septal defects were repaired with patches of Dacron in 12 cases and autologous pericardium in 54 cases. Coronary sinus was situated on the left atrium in 5 and ostium primum atrial septal defects were repaired in Kirklin’s way; the others in MeGoon’way. Meanwhile other heart abnormalities were done. Results There were two early deaths (3.03%), one patient died of heart arrhythmia and one patient died of respiratory failure. Complications of total A-V block was in 2 cases. Both of them were replanted with pace makers.52 cases were followed up, followup time was 5 months to 22 years(mean follow-up 15 years). All patients had better life. Four patients have been re -operated for different reasons post primary operation. One had good result after re-mitral valve replacement. One case died of acute renal failure and the other two died of low cardiac output syndrome. Conclusions Early operation is definitely recommended when the diagnosis is confirmed. Because the structure or function of mitral valve is saved, pulmonary hypertension is avoided and the mortality is lower in the future. The key points of operation are to rectify the mitral insufficiency, repair ostium primum atrial septal defects and avoid atrioventricular block. The patients of mild regurgitation of mitral valve have good results. Provided in those have more than middle regurgitation of mitral valve then their longterm results are poor.

    Release date:2016-08-30 06:05 Export PDF Favorites Scan
  • 胸腺瘤合并重症肌无力及单纯红细胞再生障碍性贫血二例

    Release date:2016-08-30 06:23 Export PDF Favorites Scan
  • The Short and Medium Term Effect on Myocardial Contractile Force after Implantation of Autologous Endothelial Progenitor Cells

    Objective To study the short and medium term effect of myocardial contractile force by implantation of endothelial progenitor cells (EPCs) in the myocardial infarction model. Methods Hundred and twenty SD rats were equally and randomly divided into experimental group and control group (60 rats in each group). Acute myocardial infarction model was created by ligation of LAD. Autologous EPCs were purified from peripheral blood then implanted into the acute myocardial infarct site via topical injection. IMDM were used in control group. Specimens and muscle strip were harvested at 3, 6 weeks, 6, 8 and 12 months after EPCs implantation for contractile force study and to detect the expression of vascular endothelial growth factor(VEGF), basic fibroblast growth factor (bFGF) and Ⅷ factor by immunohistology and video image digital analysis system. Results The expression of VEGF, bFGF and the microvessel counts in experimental group were much higher than those of control group(P〈 0.01) at 3, 6 weeks and 6 months after transplantation. The contractile force in experimental group was better than that in control group(P〈0.01) at the same time. But from 8 months after implantation, the contractile force and so on were not up in the experimental group. Conclusion EPCs, after being implanted into infarct myocardium, shows the ability of improvement of the contractile performance in infarcted myocardium by means of angiogenesis and vasculogenesis and the medium term results are persistent.

    Release date:2016-08-30 06:23 Export PDF Favorites Scan
  • The influence of autologous bone mesenchymal stem cells on myocardial structure and cardiac function after being implantated into acute infarcted myocardium

    Objective To study the influence of autologous bone mesenchymal stem cells (BMSCs) on myocardial structure and cardiac function after being implantated into acute infarcted myocardial site. Methods Bone marrow was aspirated from the posterosuperior iliac spine of Guizhou Xiang swine. After being isolated, cultured and co cultured with 5 azacytidine, either autologous BMSCs (total cells 2×10 6, experimental group, n =12), or a comparable volume of culture medium (control group, n =12), was injected into the left anterior descending(LAD) branch of coronary artery just distal to the ligation site of the LAD. The same volume of BMSCs or culture medium was injected into several spots in the infarcted myocardium. Echocardiographic measurements were performed three or six weeks after implantation to assess the myocardial structure and cardiac function. Results Left ventricular function, including eject fraction(EF), fractional shortening and wall thickening, were higher in experimental group when compared with control group. The thickness of the ventricular wall and septum was also found increased while the left ventricular chamber size was smaller in experimental group. Conclusion Implantation of BMSCs into the infarcted myocardium is believed to attenuate the remodeling process, inhibit the extent of wall thinning and dilatation of the ventricular chamber. BMSCs implantation may also improve the contractile ability of the myocardium and cardiac function.

    Release date:2016-08-30 06:27 Export PDF Favorites Scan
  • Angiogenesis and cytokine secretion induced by autologous bone marrow mesenchymal stem cells implantation into infarcted myocardium

    Objective To study the effect of bone marrow mesenchymal stem cells(MSCs) implantation into infarcted myocardium on cytokine secretion and angiogenesis. Methods 24 Guizhou xiang porcine were equally divided into experimental group and control group randomly. Three ml bone marrow was extracted from the posterior superior iliac spine. MSCs were cultured according to the methods of Wakitani’s. After being co-cultured with 5-azacytidine for 24 hours, these cells were labeled with bromodeoxyuridine(BrdU). Autologus MSCs were implanted into the acute myocardial infarct site both via the distal segment of the ligated left anterior descending artery (LAD) and topical injection. 3 amp; 6 weeks after transplantation, the samples from experimental group and control group were collected to detect the expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and Ⅷ factor by immunohistology and video image digital analysis system. Results The expression of VEGF, bFGF and the microvessel counts in the experimental group were much higher than those of control group (Plt;0.01) at 3 and 6 weeks after transplantation. Conclusion MSCs, after being implanted into infarcted myocardium, shows the ability of secreting VEGF, bFGF, with subsequent angiogenesis.

    Release date:2016-08-30 06:27 Export PDF Favorites Scan
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