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find Author "李丹青" 4 results
  • 肿瘤干细胞分离方法研究进展

    肿瘤干细胞是肿瘤治疗新理论,近年来受到广泛关注,也备受争议。该理论认为肿瘤干细胞是肿瘤细胞的来源,是肿瘤发生耐药、复发、转移的根本原因。目前通过悬浮细胞培养法、流式细胞分选法、免疫磁珠分选法等多种方法,已从多种实体瘤细胞系或组织中分离和鉴定出肿瘤干细胞,包括乳腺癌、脑肿瘤、结肠癌、胰腺癌、卵巢癌等,研究其生物学特性、特异性标志物及调控机制,有望为肿瘤治疗提供新的思路和策略。研究肿瘤干细胞,首先要对其分离和培养,现就目前肿瘤干细胞的分离方法研究进展予以综述。

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  • Effectiveness of Auto-CPAP versus Fixed-CPAP for the Treatment of Obstructive Sleep Apnea Syndrome: A Meta-analysis

    Objective To assess the effectiveness of the auto-continuous positive airway pressure (Auto-CPAP) versus the fixed-continuous positive airway pressure (Fixed-CPAP) in patients with obstructive sleep Apnea syndrome (OSAS). Methods Such databases as PubMed (1990 to 2010), SpringerLink (1995 to 2010), CNKI (1990 to 2010), WanFang Data (1995 to 2010), and Google academic (1994 to 2010) were searched, the relevant conference theses were retrieved, and the experts in this field were enquired to collect the randomized controlled trials (RCTs) on Auto-CPAP versus Fixed-CPAP for patients with OSAS. Two reviewers independently screened the trials according to inclusion and exclusion criteria, abstracted the data, and assessed the methodology quality. Meta-analyes was performed using RevMan 5.0 software. Results A total of 11 RCTs involving 327 patients were included. The results of meta-analyses showed that, compared with the Fixed-CPAP group after treatment, the Auto-CPAP group significantly reduced the mean effective therapeutic pressure (WMD=-1.79, 95%CI -3.39 to -0.20), won much better treatment adherence (WMD=0.43, 95%CI 0.30 to 0.56), but got much higher scores of the Apnea-hypopnea index (AHI) (WMD=1.17, 95%CI 0.25 to 2.08) and Epworth Sleepiness Scale (ESS) (WMD=0.88, 95%CI 0.42 to 1.33) as well. There was no significant difference between those two groups in patients’ subjective preference for treatment (OR=2.06, 95%CI 0.46 to 9.10). Conclusion Compared to the Fixed-CPAP, the Auto-CPAP significantly reduces the mean effective therapeutic pressure and improves the treatment adherence of the patients, but is inferior in decreasing AHI and ESS. However, more high-quality and large-scale RCTs are required to verify the above conclusion because of the limitation of research quality and sample at present.

    Release date:2016-09-07 11:06 Export PDF Favorites Scan
  • Bibliometric Analysis of Sleep Apnea Syndrome Based on Science Citation Index

    Objective To reveal the worldwide research status and hot topics of sleep apnea syndrome ( SAS) . Methods Articles were searched from Web of Science ( SCI) , Essential Science Indicator ( 2000 to 2010) database using sleep apnea syndrome or apnea as keywords. Retrieved documents were analyzed using the database with its own statistical functions and histcite software ( version 8.12. 16) .Results Since 1992 the international scientific papers on the SAS study showed a gradual upward trend.The United States is a world leader in this field. Recent research has focused on vascular endothelial barrier function and repair, oxidative stress, inflammation, cognitive function, special populations such as the elderlyor children patients with SAS. Conclusion Clinical researchers have paid more attention to SAS than before, but there are still many important issues unresolved.

    Release date:2016-08-30 11:56 Export PDF Favorites Scan
  • 难治性子宫动静脉瘘一例

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