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"杨辰垣" 4 results
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目的在非体外循环下建立猪急性StanfordB型主动脉夹层模型,为临床探讨主动脉夹层治疗方法提供实验手段。方法将10头小猪分为两组,均在非体外循环下进行。组(n=4)通过主动脉侧壁钳钳夹部分降主动脉,切开主动脉侧壁,分离主动脉中、内1/3管壁,通过升高血压冲击主动脉壁,建立急性StanfordB型主动脉夹层模型;组(n=6)先建立猪左颈动脉-左股动脉转流,完全阻断降主动脉,其余步骤同组。结果组未形成夹层及假腔,组除1头猪因术中气管插管脱出而窒息死亡,其余5头主动脉夹层均形成,剪开主动脉壁,见夹层及假腔向远端扩展达膈肌水平,长度约14～18cm,光学显微镜下观察,见夹层在主动脉中层产生。结论通过预置左颈动脉-左股动脉转流,完全阻断降主动脉,机械分离主动脉壁,利用升高血压冲击主动脉壁,造成夹层分离,可以成功建立猪急性StanfordB型主动脉夹层模型。
Release date：2016-08-30 06:25
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Objective To investigate the protection effects of different pH N 2 hydroxyethyl piperazine N′ 2 ethanesulfonic acid (HEPES) Krebs Henseleit (KH) reperfusate solutions on immature myocardium and myocardial interstitium. Methods Isolated perfused Langendorff model from immature rabbit hearts were performed. Twenty four rabbits were divided into control group, ischemia reperfusion group and acidic reperfusate group. The left ventricular function recovery, myocardial water content(MWC), lactate dehydrogenase (LDH) and creatine kinase (CK) leakage, malondialdehyde(MDA) and adenosine triphosphate (ATP) content, superoxide dismutase(SOD) activity , hydroxyproline(HP) and endothelin(ET) content were tested. Results The left ventricular functional recovery, ATP content, SOD activity and HP content in acidic reperfusate group were higher than those of ischemia reperfusion group( P lt;0.05).MWC, MDA content, CK and LDH leakage, and ET content in acidic reperfusate group were lower than those of ischemia reperfusion group( P lt;0.05). Conclusion These results suggest pH paradox might be one of important mechanisms for immature myocardium and myocardial interstitium ischemia reperfusion injury,and acidic perfusate, at the beginning of reperfusion ,might attenuate pH paradox and ameliorate functional recovery in isolated perfused immature rabbit hearts.
Release date：2016-08-30 06:27
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目的 研究非心脏缺血预处理(IP)对未成熟心肌的保护作用,探讨未成熟心肌的保护方法. 方法 采用心脏缺血预处理(MIP)、双下肢缺血预处理(DLIP)和肾缺血预处理(RIP)兔Langendorff灌注模型,比较3种方法对缺血-再灌注(IR) 未成熟心肌损伤的效应.将24只兔分为4组,IR组,MIP组,DLIP组和RIP组,每组6只.测定左心室功能恢复、心肌含水量(MWC)、乳酸脱氢酶(LDH)漏出量、血清肌酸激酶(CK)漏出量、心肌三磷酸腺苷(ATP)、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性等指标. 结果 MIP组、DLIP组和RIP组左心室功能恢复指标、ATP含量和SOD活性优于IR组(P＜0.05),MWC低于IR组(P＜0.05),LDH、CK漏出率和MDA含量均低于IR组(P＜0.01). 结论 非心脏IP对未成熟心肌具有明显的保护作用,与MIP相同,可诱发同等效应的心肌保护作用.
Release date：2016-08-30 06:32
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目的 探讨三甲氧苄嗪增补于心脏停搏液中对离体鼠心再灌注损伤的保护作用. 方法 将24只Wistar大鼠随机分为实验组和对照组.离体鼠心在改良的Langendorff-Neely灌注模型上30分钟预灌注,120分钟停搏,30分钟再灌注.缺血前和再灌注期间测定血流动力学指标、心肌酶中血清肌酸激酶(CK)、血清乳酸脱氢酶(LDH)、心肌超氧化物歧化酶(SOD)、过氧化脂质(LPO)含量、心肌三磷酸腺苷(ATP)水平.电子显微镜观察心肌超微结构. 结果 再灌注后,实验组心功能、心肌超微结构的改善明显优于对照组,CK、LDH和LPO含量显著低于对照组(Plt;0.01),SOD含量和心肌ATP水平显著高于对照组(Plt;0.01). 结论 三甲氧苄嗪增补于心脏停搏液中可显著减轻心肌缺血-再灌注损伤,具有良好的心肌保护作用.
Release date：2016-08-30 06:32
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