【摘要】 目的 比较酸性成纤维细胞生长因子(acid fibroblast growth factor,aFGF或FGF1)在正常乳腺组织、乳腺良性肿瘤及乳腺癌中的表达差异,探讨FGF1与乳腺癌血管生成的关系。方法 应用免疫组织化学(immunohistochemistry,IHC)SP法检测FGF1在40例乳腺癌组织、12例良性乳腺肿瘤组织及12例正常乳腺组织中的表达情况；以CD34抗体标记血管内皮细胞CD34抗原行乳腺癌组织微血管密度(micro vessel density,MVD)计数。〖HTH〗结果〖HTSS〗 FGF1在40例乳腺癌中的阳性表达率（57.5%，23/40)显著高于12例乳腺良性肿瘤组织中阳性表达率(16.7%,2/12)以及正常乳腺组织阳性表达率(0,0/12),差异有统计学意义(Plt;0.05)；良性肿瘤组FGF1表达率和正常乳腺组织比较无统计学意义(Pgt;0.05)。40例乳腺癌组织MVD计数为(70.17±29.33)个/HP,在23例FGF1阳性组中MVD计数为(89.48±23.23)个/HP,显著高于17例阴性组(44.06±12.53)个/HP,差异有统计学意义(Plt;0.05)。〖HTH〗结论 〖HTSS〗FGF1可能参与乳腺癌微血管生成。
Objective To explore and implement a systematic, case guided online interactive training course for neurologists to improve their diagnosis and treatment of rare genetic diseases. Methods Doctors who participated in the course investigation of the neurogenetic project of the Department of Neurology of Peking Union Medical College Hospital between January and September 2021 were selected. Based on andragogy theory, a genetics training course for neurologists was developed by applying Kern’s six steps of curriculum development. According to the time of participating in the doctor’s courses, they were divided into three groups: completed all courses (10.7 h group), completed more than 1/2 courses (5.3～10.7 h group) and completed less than 1/2 courses (<5.3 h). According to the length of service, they were divided into groups of less than 10 years, 10-20 years and more than 20 years. Analyze the benefit difference of different doctors’ training time, and collect their feedback scales on the curriculum for the improvement of follow-up courses. Results A total of 54 doctors were included. Among them, 17 (31.5%) completed all courses, 29 (53.7%) completed more than 1/2 courses, and 8 (14.8%) completed less than 1/2 courses. There was a statistically significant difference among the three groups in the self-assessment improvement score (H=12.341, P=0.002). The results of pairwise comparison between groups of self-assessment improvement score showed that the <5.3 h group was lower than that of the 10.7 h group (P=0.007), and the the <5.3 h group was also lower than that of the 5.3～10.7 h group (P=0.002). 33 (61.1%) in the less than 10 years group, 16 (29.6%) in the 10-20 years group, and 5 (9.3%) in the more than 20 years group. There was no correlation between participating in work and course time (rs=0.113, P=0.418). 54 (100.0%) believed that they had more than moderate help (≥3 points). Most doctors (>90%) had a good evaluation of the curriculum. Conclusion The periodic neurogenetic re-education project is helpful for clinical diagnosis and treatment of rare neurogenetic diseases.