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find Author "梁峰" 3 results
  • 颈椎椎管内/ 外骨软骨瘤一例

    略。。。

    Release date:2016-08-31 05:49 Export PDF Favorites Scan
  • EXPERIMENTAL STUDY ON CHONDROGENIC DIFFERENTIATION OF RABBIT ADIPOSE-DERIVED STEM CELLS TREATED WITH GROWTH DIFFERENTIATION FACTOR 5

    Objective To investigate the feasibil ity and effect of inducing adi pose-derived stem cells (ADSCs) treated with growth differentiation factor 5 (GDF-5) to undergo chondrogenic differentiation in vitro. Methods Six healthy Japanese rabbits aged 3 months (2-3 kg) of clean grade were chosen, irrespective of sex. ADSCs were isolated and cultured with collagenase digestion, then were detected and identified by vimentin immunohistochemistry and CD44, CD49d, CD106immunofluorescence staining. ADSCs at passage 3 were used and the cell density was adjusted to 1 × 106/mL, then the ADSCs were treated with 0, 10, 100 ng/mL GDF-5 and common cultural medium, respectively. The morphology changes of the induced ADSCs were observed by inverted contrast phase microscope and their growth state were detected by MTT. The mRNA quantities of Col II and proteoglycan expressed by the induced ADSCs were detected with RT-PCR. The Col II proteoglycan synthesized by the induced ADSCs were detected with alcian blue staining, toluidine blue staining, immunohistochemistry staining, and Western blot method. Results ADSCs mostly presented small sphere, fusiform and polygon shape with positive expression of CD44 and CD49d and negative expression of CD106 and vimentin. The ADSCs treated with 100 ng/mL GDF-5 presented sphere or sphere-l ike change and vigorous prol iferation. The mRNA quantities of Col II and proteoglycan synthesized by the induced ADSCs treated with 0, 10, 100 ng/mL GDF-5 and common cultural medium increased in a dose-dependent manner at 7 days. There were significant differences among all the groups (P lt; 0.05), except that no significant difference was evident between the 0 ng/mL group and the 10 ng/mL group (P gt; 0.05). When ADSCs were treated with 100 ng/mL GDF-5 for 14 days, the Col II and the mRNA and protein quantities of ptoteoglycan reached the peak, and the results of alcian blue, toluidine blue and Col IIimmunohistochemistry staining were positive. Conclusion ADSCs treated with certain concentration of GDF-5 have higher expression of Col II and proteoglycan and possess partial biological function of chondrocyte.

    Release date:2016-09-01 09:05 Export PDF Favorites Scan
  • 一期前路病灶清除植骨及内固定术治疗颈椎及颈胸段脊柱结核

    目的 总结一期前路病灶清除、椎体间植骨及前路内固定治疗颈椎及颈胸段脊柱结核的临床疗效,探讨重建脊柱稳定性的必要性和安全性。 方法 2002 年4 月- 2006 年3 月,采用一期前路病灶清除、椎体间植骨及前路内固定治疗13 例颈椎及颈胸段脊柱结核患者。男8 例,女5 例;年龄21 ~ 58 岁。病程1 ~ 7 个月,平均4 个月。颈椎结核10 例,颈胸段结核3 例。术前X 线片、CT、MRI 检查示病变部位为:C3、4 1 例,C5 2 例,C5、6 3 例,C6、7 4 例,C7、T12 例,C7 ~ T2 1 例。后凸Cobb 角为20 ~ 50°,平均35.7°。神经功能ASIA 分级:B 级1 例,C 级4 例,D 级6 例,E 级2 例。术前血沉34 ~ 78 mm/h,平均42 mm/h。 结果 术后患者均获随访,随访时间9 ~ 34 个月,平均14 个月。均未出现伤口深部感染或窦道形成,平均1.5 个月血沉降至20 mm/h 以下。患者植骨均完全融合,融合时间3 ~ 5 个月,平均3.4 个月。术后后凸Cobb 角17 ~ 39°,平均29.3°;随访14 个月时为9 ~ 21°,平均14.5°。神经功能除1 例B 级恢复至D 级外,余均达E级。 结论 一期前路病灶清除同期植骨内固定治疗颈椎及颈胸段脊柱结核能彻底清除病灶、防止复发、矫正畸形、重建脊柱稳定性,促进脊柱植骨融合,提高脊柱结核的治愈率。

    Release date:2016-09-01 09:17 Export PDF Favorites Scan
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