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find Author "毕锋" 17 results
  • 人表皮生长因子受体 2 在胃癌中的研究进展

    胃癌是我国常见的恶性肿瘤,晚期胃癌患者预后差,治疗疗效差,分子靶向治疗研究无疑将为晚期胃癌患者带来新的曙光。ToGA 研究为第一个获得晚期胃癌靶向治疗成功的临床研究,其将抗人表皮生长因子受体 2(human epidermal growth factor receptor,HER2)单克隆抗体曲妥珠单抗与化学疗法联合应用于晚期胃癌一线治疗。在 ToGA 研究之后,学者们对于胃癌抗 HER2 通路治疗的研究并未停止脚步,在精确筛选 HER2 阳性患者、HER2 阳性与患者预后、新的抗 HER2 治疗策略、治疗耐药机制及克服耐药策略等多方面取得了许多研究进展。该文针对 ToGA 研究之后胃癌 HER2 通路治疗的相关进展进行了综述。

    Release date:2017-08-22 11:25 Export PDF Favorites Scan
  • 肾脏癌肉瘤一例

    Release date:2016-08-26 02:21 Export PDF Favorites Scan
  • 表皮生长因子受体单克隆抗体耐药机制研究的新进展

    表皮生长因子受体(EGFR)是HER家族中一类表皮生长因子酪氨酸激酶受体,其下游的信号通路调控着细胞的生长、增殖、分化、侵袭、迁移、血管形成等生物学行为。EGFR在上皮源性肿瘤中常有表达,在近几十年中,将EGFR作为抗肿瘤治疗的靶点,研制出一系列靶向治疗药物,主要有小分子酪氨酸激酶抑制剂和单克隆抗体这两大类。然而,在结直肠癌中EGFR单克隆抗体靶向治疗的有效率却不高,原发和继发性耐药成为阻碍靶向药物应用的关键因素。由此推动了关于靶向药物各种耐药机制的研究,现就近两年来EGFR单克隆抗体耐药机制研究的新进展作一综述。

    Release date:2016-09-07 02:38 Export PDF Favorites Scan
  • The Effects of RhoE siRNA on the Malignant Phenotypes of Breast Cancer

    目的 观察下调Ras同源类似物E (RhoE)表达对人乳腺癌细胞231生物学行为的影响。 方法 蛋白质印迹技术检测小干扰RNA(siRNA)转染前后RhoE在乳腺癌细胞231中的表达;RhoE siRNA的细胞转染 用lipofectamine?2000脂质体法;Cell Counting Kit-8检测转染细胞及对照细胞的增殖变化;损伤刮擦试验和体外侵袭实验(Transwell小室)分别检测转染细胞及对照细胞的迁移与侵袭能力。 结果 RhoE在乳腺癌细胞231中的表达较高;成功转染RhoE siRNA的乳腺癌细胞,蛋白质印迹显示RhoE的表达被明显的抑制;RhoE的表达被抑制后对乳腺癌细胞的增殖、迁移和侵袭有着明显的促进作用。 结论 下调RhoE 表达能够明显促进乳腺癌细胞的增殖﹑迁移和侵袭,RhoE可能在乳腺癌的发生发展中起着重要作用。

    Release date:2016-09-08 09:13 Export PDF Favorites Scan
  • 血管肌纤维母细胞瘤1例并文献复习

    目的:检索有关血管肌纤维母细胞瘤的文献。方法:检索中国期刊全文数据库、中文期刊全文数据库、万方数字化期刊全数据库。结果:检索的所有文献中血管肌纤维母细胞瘤均以个案报道,主要为女性,发病部位大多位于外阴,其次为阴道、宫颈、腹股沟,也有男性阴囊、腹股沟以及1例口底区的报告。结论:血管肌纤维母细胞瘤多为良性病变,彻底手术切除后无复发,但也有复发及恶变报道,应做长期随访观察。

    Release date:2016-09-08 10:00 Export PDF Favorites Scan
  • 成人肾恶性横纹肌样瘤1例

    Release date:2016-09-08 10:01 Export PDF Favorites Scan
  • Research Progress of microRNA Influences on Colorectal Cancer

    microRNA (miRNA) is a kind of RNAs which involves in the regulation of proliferation, invasion and metastasis of tumors. It also closely connects to the colorectal cancer. This article reviews the relationship between miRNA and proliferation, invasion, metastasis, diagnosis and treatment of colorectal cancer.

    Release date:2017-01-17 06:17 Export PDF Favorites Scan
  • A β-catenin/IQGAP1 regulatory feedback loop and its effects on the proliferation of colon cancer cells

    The aim of this article is to study the regulatory feedback loop between β-catenin and IQ motif containing GTPase activating protein 1 (IQGAP1), as well as the effect of this regulation loop in colon cancer cell proliferation. Western blot was used to detect the expression of IQGAP1 and β-catenin after changing their expression respectively by transfection in SW1116 cells. CCK-8 cell proliferation assay was used to detect the effect of IQGAP1 involved in the proliferation of SW1116 cells promoted by β-catenin. The results of Western blot indicated that β-catenin could positively regulate IQGAP1, while IQGAP1 silencing could up-regulate β-catenin, forming a negative feedback loop. The results of CCK-8 showed that IQGAP1 silencing inhibited β-catenin-mediated proliferation in SW1116 cells. In conclusion, our research reveals a negative regulatory feedback loop between β-catenin and IQGAP1 which has a remarkable effect on the proliferation ability of colon cancer cells.

    Release date:2018-02-26 09:34 Export PDF Favorites Scan
  • Comprehensive analysis of the aberrantly expressed profiles of lncRNAs, miRNAs and the regulation network of the associated ceRNAs in clear cell renal cell carcinoma

    To evaluate the differential expression profiles of the lncRNAs, miRNAs, mRNAs and ceRNAs, and their implication in the prognosis in clear cell renal cell carcinoma (CCRCC), the large sample genomics analysis technologies were used in this study. The RNA and miRNA sequencing data of CCRCC were obtained from The Cancer Genome Atlas (TCGA) database, and R software was used for gene expression analysis and survival analysis. Cytoscape software was used to construct the ceRNA network. The results showed that a total of 1 570 lncRNAs, 54 miRNAs, and 17 mRNAs were differentially expressed in CCRCC, and most of their expression levels were up-regulated (false discovery rate < 0.01 and absolute log fold change > 2). The ceRNA regulatory network showed the interaction between 89 differentially expressed lncRNAs and 9 differentially expressed miRNAs. Further survival analysis revealed that 38 lncRNAs (including COL18A1-AS1, TCL6, LINC00475, UCA1, WT1-AS, HOTTIP, PVT1, etc.) and 2 miRNAs (including miR-21 and miR-155) were correlated with the overall survival time of CCRCC (P < 0.05). Together, this study provided us several new evidences for the targeted therapy and prognosis assessment of CCRCC.

    Release date:2019-04-15 05:31 Export PDF Favorites Scan
  • 胰腺神经内分泌肿瘤的治疗进展

    胰腺神经内分泌肿瘤(PNET)是来源自胰腺多能神经内分泌干细胞的一种罕见的胰腺肿瘤,临床表现复杂多样,病程缓慢, 最终发生转移致死。PNET分为功能性和非功能性,目前治疗有手术、化学疗法、放射治疗、介入、生物治疗以及分子靶向药物治疗,近年生物治疗及分子靶向药物治疗在研究中显示出了良好疗效。根据国内外文献及最新临床试验研究进展,现就PNET治疗进展进行综述。

    Release date:2016-09-08 09:11 Export PDF Favorites Scan
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