Purpose Photoreceptor cells transplantation was used to determine the localization of the amino acid neurotransmitters (gamma;-aminobutyrate，GABA) in royal college of surgeons rat (RCS rat)'s retina． Methods The healthy Wistar ratsphotorec eptor cells were grafted into the subretinal space of the left eye of the RCS rat by outer path way． Two weeks later,host animals were sacrificed． Both sides of eyes were processed for light microscopic analysis． So did the 74 days Wis tar rats and 74 days RCS rats．Then the method of immunocytochemistry was used to determine the localization of the amino acid neurotransmitters GABA．We gauge the quantity of the amacrine cells and the optical density of inner plexiform layer (IPL) and ganglion cell layer (GCL) of the four groups．ANOVA test were complied to determine significant difference between them． Results After two weeks of transplantation, the transplanted photor eceptor cells survived and the outer plexiform layer was rebuilt ．There are significant differences (Plt;0．001) in the level of the GABA immunoreactivity (IR) within the IPL between the grafted RCS rats eyes and the ungrafted RCS rats eyes． There are differences (ANOVA, Plt;0 ．05) of the quantity of the GABAenergetic amacrine cells among the grafted RCS rats eyes, the ungrafted RCS rats eyes and the Wistar rats． But there was no difference of the quantity of the GABAenergetic amacrine cell s among the grafted RCS rats retina． Conclusio These results suggest that the level of GABA neurotr ansmitters in the reconstructed retina is almost normal, which indicate the func tion of the morbid retina may be resumed by transplanting the photor eceptor cells． (Chin J Ocul Fundus Dis, 2001,17:128-131)
Objective To evaluate the diagnostic value of serum pro-gastrin-releasing peptide (Pro-GRP) in patients with small cell lung cancer. Methods We searched MEDLINE, EMBASE, The Cochrane Library and other databases (1966 to Sept 2009) to collect studies which evaluated the diagnostic value of Pro-GRP in patients with small cell lung cancer. The heterogeneity of the included studies was tested by the Cochrane Collaboration’s software RevMan 4.2. The Summary Receiver Operating Characteristic (SROC) curve and meta-analyses were performed by MetaDisc. Results A total of 256 relevant articles were retrieved and 19 were included in our review. Eleven studies involving 1 447 patients were included. Meta-analyses showed that the heterogeneity among studies was high (P﹤0.000 01, I2=69.3%), the pooled sensitivity was 0.717 and the pooled specificity was 0.963. Subgroup analyses indicated that 9 of the studies which used the LD (Limited diseases) SCLC group (P=0.003, I2=65.5%, SEN=0.637, SPE=0.968, SROC AUC=0.724 3) had heterogeneity and ED (Extensive diseases) SCLC group (P=0.2, I2=27.0%, SEN=0.766, SPE=0.968, SROC AUC=0.935 5) had no heterogeneity. And 15 of the studies of Pro-GRP which were determined by acmmercial sandwich ELISA (Japan) group (P=0.000 1, I2=68.5%) had heterogeneity. Three of the studies of Pro-GRP which were determined by ELISA (Germany) group (P=0.948 7, I2=0.001%) had no heterogeneity. Conclusion Pro-GRP could be regarded as one of the reference tests in patients with small cell lung cancer, but higher quality trials are required.