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PROGRESS IN ISOLATION AND PURIFICATION OF PORCINE ISLETS

Objective To review the common methods of isolation and purification of porcine islets and research progress. Methods Domestic and abroad literature concerning the isolation and purification of porcine islets was reviewed and analyzed thoroughly. Results The efficacy of the isolation and purification depends on the selection of donor, the procurement and cryopreservation of high-quality donor pancreas, and the selection and improvement of the operation. Conclusion The shortage of transplanted islets could be resolved by the establishment of standardized and optimal process, which may also promote the development of porcine islet xenograft.

掌背动脉蒂逆行岛状组织瓣在手外伤中的应用

Analysis of Effect and Quality of Laparoscopic Cholecystectomy with Common Bile Duct Exploration，Built-in-Tube Drainage, and Primary Suture

ObjectiveTo compare and evaluate the effect and quality of T-tube drainage and bulit-in-tube drainage plus primary suture after laparoscopic cholecystectomy (LC). MethodsA clinical trial was taken in 79 cases with T-tube drainage (control group) and 62 cases with built-in-tube drainage (observation group). The treatment success rate, incidence of complications, bilirubin recovered time, length of stay, recuperation time, and treatment cost were measured and compared between the two groups. ResultsThere were no statistically significant differences between the two groups in treatment success rate, incidences of complications, and bilirubin recovered time of patients （Pgt;0.05）, while length of stay, recuperation time, and treatment cost of patients in observation group were significantly less than those in control group （Plt;0.05）. ConclusionsBuilt-in-tube drainage plus primary suture after LC and common bile duct exploration could achieve the same therapeutic effect as the traditional T-tube drainage with less length of stay, recuperation time, and treatment cost.

天玑骨科机器人辅助四角融合经皮螺钉内固定技术介绍

Imaging diagnosis and research progress of gastric cancer in peritoneal metastasis

Gastric cancer remains one of the most prevalent and fatal malignancies in China. Peritoneal metastasis represents a frequent mode of dissemination or recurrence in patients with advanced disease and confers an extremely poor prognosis. In recent years, considerable progress has been made in imaging techniques, with modalities including CT, ultrasound, MRI and PET-CT being implemented to evaluate peritoneal metastasis. However, adequate detection remains challenging, particularly for occult peritoneal metastasis. With the advent of precision medicine, radiomics and artificial intelligence have undergone rapid development and show considerable promise for the early prediction of peritoneal metastasis in gastric cancer, providing a new means of diagnosis and treatment for patients with peritoneal metastasis.

Analysis on Effect of Neoadjuvant Chemotherapy of Different Differentiated Grades of Locally Advanced Gastric Cancer

ObjectiveTo evaluate the influence of pathological differentiation in the effect of preoperative chemo-therapy for patients with locally advanced gastric cancer (LAGC). MethodsThirty-two patients with LAGA received preoperative chemotherapy with oxaliplatin and capecitabine (XELOX regimen).According to the pathological examina-tion, patients were classified into better (well and moderate, 16 cases) and poorly (16 cases) differentiated groups, and the clinical response rate, type of gastrectomy, and negative tumor residual rate were compared between the two groups.Morphological changes and toxic reactions were monitored after chemotherapy. ResultsThe results showed that the clinical response rate in the better differentiated group was significantly higher than that in the poorly differentiated group (100% vs.6.4%, P=0.000).The partial gastrectomy rate in the better differentiated group was significantly higher than that in the poorly differentiated group (87.5% vs.25.0%, P=0.000).A significant shrinking of tumor size and necrosis of tumor tissues caused by chemotherapy could be observed. ConclusionThe better differentiated group with locally advanced gastric cancer is suitable for preoperative chemotherapy with XELOX regimen, and as a result of effective preoperative chemotherapy, much more gastric tissue can be preserved for better differentiated group.

A comparative study of nasal and pharyngeal swabs in the diagnosis of coronavirus disease 2019

ObjectiveTo investigate a more convenient and safe sampling method for viral nucleic acid detection of coronavirus disease 2019.MethodsAn oropharyngeal swab and nasopharyngeal swab were simultaneously taken from 100 patients with coronavirus disease 2019 in a hospital in Wuhan. Then the efficacies of two sampling methods were compared on the positive rates of viral nucleic acid detection.ResultsThe positive rate for SARS-CoV-2 was 54% in oropharyngeal swabs, while 89% positive in nasopharyngeal swabs. There was a significant difference in the detection rate between oropharyngeal swab and nasopharyngeal swab (χ2=3.850 4, P=0.049 7).ConclusionsThe positive rate for nucleic acid testing from nasopharyngeal swabs are significantly better than that from oropharyngeal swabs. Therefore, sampling by nasopharyngeal swabs, rather than oropharyngeal swabs, should be chosen as the preferred virological screening method for patients with coronavirus disease 2019.

One Case Report of Abdominal Cocoon in Laparoscopic Cholecystectomy and Review of The Literatures

目的 系统阐述腹茧症的发病机理、临床表现和诊治进展。方法 报道腹腔镜胆囊切除术中意外遭遇腹茧症1例，并进行文献综述。 结果 该例患者在腹腔镜下完成胆囊切除并切除膜状组织，术后病理诊断为纤维结缔组织增生伴慢性炎症反应，术后患者恢复顺利。结论 腹茧症的症状、体征及辅助检查均无特异性，较易出现误诊或漏诊，而腹腔镜技术的发展为该疾病的诊治提供了新的尝试。

Comparison of short-term effect between laparoscopic and open Miles operations for lower rectal carcinoma

Objective To evaluate short-term effect of laparoscopic and open Miles operations for lower rectal carcinoma. Methods A total of 119 patients with lower rectal carcinoma were retrospectively collected from March 2012 to March 2017 in this hospital, among which 65 were in the laparoscopic operation group and 54 in the open operation group. The perioperative data, pathological results, recovery courses, and complications were compared between the two groups. Results Compared with the open operation group, the laparoscopic group showed a longer operation time (t=6.035, P=0.002), quicker bowel function recovery (t=4.919, P<0.001), faster off-bed activity (t=2.221, P<0.001), and shorter hospital stay time (t=3.795, P=0.025). The intraoperative blood loss (t=0.154, P=0.698) and the number of harvested lymph nodes (t=0.532, P=0.595) were similar between the two groups. The laparoscopic operation group showed a significant lower total complication rate (χ2=7.174, P=0.009) as compared with the open operation group, but the incision infection, urinary tract injury, lung infection, thrombosis of lower extremities, etc. had no significant differences between the two groups (P>0.050). Conclusion Laparoscopic Miles operation improves postoperative recovery and reduces postoperative complications as compared with open approach in treatment of lower rectal cancer, with similar oncological and short-term results.

新型冠状病毒肺炎隔离病房患者嗅觉减退原因的多因素分析

目的探讨住院新型冠状病毒肺炎（简称新冠肺炎）患者嗅觉减退情况及原因。方法对新冠肺炎定点收治医院隔离病房的患者展开问卷调查，了解患者年龄、性别、文化程度、患病时间、既往嗅觉灵敏程度、本次患病有无上呼吸道症状（鼻塞、流涕及打喷嚏等）、既往感冒史及感冒时有无嗅觉减退；通过主观判断法，使用家庭常见的白醋，重新单独包装后让患者闻嗅，判断患者嗅觉是否减退或丧失。采用多因素的二分类 Logistic 回归分析新冠肺炎患者嗅觉减退的可能原因。结果共调查 79 例患者，其中 14 例（17.72%）发生不同持续时间的嗅觉减退。二分类 Logistic 回归分析结果显示，新冠肺炎患者嗅觉减退或丧失与患者年龄、文化程度、基础疾病、治疗药物、上呼吸道症状无明显关联（P>0.05）。女性患者较男性患者更容易发生嗅觉减退（P=0.038）；患病时间越长，越容易发生嗅觉减退（P=0.033）；不吸氧患者较吸氧患者更容易出现嗅觉减退（P=0.048）。结论新冠肺炎患者在患病期间可以出现程度不同的嗅觉减退。多因素分析提示嗅觉减退与患者性别、患病时长以及吸氧与否有一定关系。