Objective To study the effect of water soluble chitosan (WSC) on the apoptosis of peritoneal macrophage induced by lipopolysaccharides (LPS), and discuss the mechanism. Methods Peritoneal macrophages were divided to three groups: phosphate buffered saline (PBS) group, LPS group and LPS plus WSC group. At hour 24, apoptosis cell and active caspase-3 were detected by flow cytometry; nitric oxide (NO) was determined with Griess reagent. Results There were more apoptosis cells in the LPS group than the PBS group. The percentage of apoptosis cells was significantly decreased in the LPS plus WSC group than the LPS group. The expression of active caspase-3 and the secretion of NO were also inhibited by WSC after LPS intervention. Conclusion WSC inhibits apoptosis of peritoneal macrophage induced by LPS.
目的探讨透明细胞型肝癌的诊断与治疗。方法回顾性分析了1988年4月至2001年2月我院外科收治的3例患者的临床资料。结果3例均为女性，HBsAg阳性2例。B超检查3例，CT和MRI扫描各2例，影像学诊断各不相同。均经手术行肿瘤切除治疗。手术后随访生存5年以上2例。结论早期积极采取手术切除治疗，是取得较好临床疗效的关键。