Objective To study the molecular characteristics of RNA binding protein aldehyde dehydrogenase 18 family member A1 (ALDH18A1) in esophageal carcinoma cells (KYSE150 cells) and its effect on tumor growth. MethodsHuman esophageal squamous cell (KYSE150 cells) was cultured in vitro. At the same time, RNA co-immuno precipitation technology was used to study the binding of RNA and protein in the cell, and the corresponding RNA-protein complex was precipitated by the antibody of the target protein to separate and purify the captured RNA. The molecular characteristics of ALDH18A1 binding RNA were analyzed, and KyotoEncyclopedia of Genes and Genomes cluster analysis was performed for ALDH18A1 binding target genes. Results Protein immunoblotting experiments showed that the target protein was well enriched by antibodies. ALDH18A1 had extensive RNA binding activity, with significant enrichment in regions such as coding sequences, intron, and 5’untranslated region. ALDH18A1 mainly bound to the UGUAAUC motif of RNA. The cluster analysis showed that the RNA molecules bound to ALDH18A1 mainly participated in focal adhesion, central carbon metabolism in cancer, cell cycle, spliceosome, RNA transport, and ubiquitin mediated protein hydrolysis. Conclusion ALDH18A1 has the function of binding to RNA molecules and may play a role in the expression of esophageal cancer-related genes and related biological processes.
目的 探讨外伤性脾破裂脾部分切除术的手术要点及术后脾功能变化。方法 对74例外伤性脾破裂行脾部分切除术者进行回顾性分析，其中不规则脾部分切除术30例，规则性脾部分切除术44例，获1～8年随访者55例。结果 ①脾创面用明胶海绵双层三叠片处理疗效肯定，②术后各种感染率低于脾脏全切者，③术后免疫功能无变化，④成人保留脾块无再生倾向。结论 脾外伤脾部分切除术疗效肯定，安全可行。只要保留有正常血循环的1/3脾脏，其免疫功能无明显影响。