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find Author "王洲" 5 results
  • 非小细胞肺癌隐匿性淋巴结转移的基因诊断

    目的 探讨非小细胞肺癌(NSCLC)隐匿性纵隔淋巴结转移病灶的基因诊断方法. 方法 应用逆转录聚合酶链反应法(RT-PCR), 检测30例NSCLC患者(实验组,N0,Ⅰa~Ⅱb期)手术后病理诊断为阴性的138枚纵隔淋巴结中MUC1基因mRNA的表达,并用30枚肺良性疾病的局部淋巴结作阴性对照(阴性对照组),用30枚经病理证实有转移的NSCLC纵隔淋巴结作阳性对照(阳性对照组).对患者进行随访,用χ2检验比较MUC1基因mRNA阳性者和阴性者的预后差别. 结果 阴性对照组30枚肺良性疾病的局部淋巴结均无MUC1基因mRNA表达(特异性为100%),阳性对照组30枚经病理证实有转移癌的肺癌纵隔淋巴结中26枚检测到MUC1基因mRNA的表达(敏感性为87%).实验组9例患者的11枚淋巴结中检测到MUC1基因mRNA表达(检出率8.0%),患者的分期上调为Ⅲa期.实验组中MUC1基因mRNA阳性患者预后不良,随访2年有4例复发、转移或死亡;MUC1基因mRNA阴性者仅1例转移(P<0.05 ). 结论 应用RT-PCR法检测纵隔淋巴结中MUC1基因mRNA的表达可以诊断肺癌隐匿性淋巴结转移.

    Release date:2016-08-30 06:32 Export PDF Favorites Scan
  • THE TREATMENT OF FRACTURE OF PATELLA BY INTERNAL FIXATION WITH TENSION BAND FROM MUSCULOFASCIAL TENDON

    In the study of the efficacy of internal fixation with tension band of musculofascial tendon in the treatment of fracture of patella, 52 cases were reported. After a following-up of 6 months to 13 months the bone healing was observed, in 7.5 weeks in average, and the function of the knee joint had recovered to normal or almost normal. It was concluded that the treatment of fracture of patella by internal fixation with tension band from musculofascial tendon was a ideal and practicable method.

    Release date:2016-09-01 11:07 Export PDF Favorites Scan
  • 外展(足母)肌皮瓣转移治疗跟骨骨髓炎

    Release date:2016-09-01 11:40 Export PDF Favorites Scan
  • Clinical study of shear wave elastography combined with diaphragm thickening fraction and rapid shallow breathing index to predict the outcome of patients with mechanical ventilation

    Objective To explore the application value of shear wave elastography (SWE) combined with diaphragmatic thickening fraction (DTF) and rapid shallow breathing index (RSBI) in predicting the results of weaning of patients with mechanical ventilation. Methods Fifty-two patients with severe illness who were hospitalized in this hospital from January 2022 to September 2022 were treated with mechanical ventilation. After meeting the conditions for weaning, they underwent spontaneous breathing test, and the diaphragm function of patients was evaluated by measuring DTF using ultrasound technology and shear modulus (SM) using SWE technology. According to the weaning results, they were divided into weaning success group and weaning failure group, The differences of mechanical ventilation time, acute physiology and chronic health evaluation Ⅱ (APACHE Ⅱ) score, respiratory rate, RSBI, oxygenation index, DTF, SM and other parameters between the two groups were compared. Multivariate logistic regression was used to analyze the factors affecting the withdrawal results. The receiver operator characteristic (ROC) curve was used to evaluate the predictive value of potential influencing factors on the withdrawal results. Results There were 39 cases of successful withdrawal and 13 cases of failure. There were significant differences in mechanical ventilation time, respiratory rate, RSBI, DTF and SM between the successful weaning group and the failure group (P<0.05). Through multivariate logistic regression analysis, RSBI [area under the ROC curve (AUC)=0.771, 95% confidence interval (CI) 0.589 - 0.953], DTF (AUC=0.806, 95%CI 0.661 - 0.951), SM (AUC=0.838, 95%CI 0.695 - 0.981) were independent factors that affected the results of withdrawal. The single parameter AUC was smaller than the combined index with RSBI≤70.48 times·min–1L–1, DTF≥30.0%, SM≥10.0 kPa as the cutoff value (AUC=0.937, 95%CI 0.714 - 1.0, diagnostic sensitivity, specificity and accuracy were 94.9%, 84.6% and 92.3% respectively). Conclusions SWE technology provides a new quantitative index for evaluating diaphragm function by evaluating diaphragm stiffness. Diaphragm stiffness combined with DTF and RSBI can better predict the successful withdrawal in patients with mechanical ventilation.

    Release date:2023-05-26 05:38 Export PDF Favorites Scan
  • EFFECTS OF 5-FLUOROURACIL ON TENDON ADHESION FORMATION AFTER FLEXOR TENDON REPAIR

    To evaluate the effect of 5-fluorouracil (5-FU) appl ied topically on preventing adhesion andpromoting functional recovery after tendon repair. Methods From August 2003 to June 2007, 48 patients with flexor tendonrupture of the fingers by sharp instrument were treated and randomly divided into two groups. In 5-FU group, 39 fingers of 26 patients included 17 males and 9 females, aged (29.3 ± 9.8) years; the locations were zone I in 19 fingers and zone II in 20 fingers; single finger was involved in 12 cases and more than 2 fingers were involved in 14 cases; and the time from injury to operation was (2.4 ± 1.6) hours. In control group, 36 fingers of 22 patients included 14 males and 8 females; aged (26.1 ± 8.7) years; the locations were zone I in 16 fingers and zone II in 20 fingers; single finger was involved in 10 cases and more than 2 fingers were involved in 12 cases; and the time from injury to operation was (2.1 ± 1.8) hours. No statistically significant difference was found in constituent ratio of age, gender, injured fingers and their zones, between two groups (P gt; 0.05). The repair site in 5-FU group was given 5-FU at a concentration of 25 mg/mL with a soaked sponge, and the synovial sheath of the repaired site was wrapped with the 5-FU-soaked sponge for 1 minute for 4 times after the tendons were repaired; normal sal ine was used in the control group. Results Wound healed by first intention and no infection and tendon rupture occurred in two groups. The patients were followed up for 3-8 months (mean 4.1 months) and 3-8 months (mean 3.9 months) in 5-FU group and in control group respectively. The functional recovery degrees of the fingers were evaluated with total active movement (TAM) evaluation system. In 5-FU group, the results were excellent in 22 fingers, good in 13 fingers, fair in 3 fingers and poor in 1 finger; the excellentand good rate was 89.7%. In control group, the results were excellent in 11 fingers, good in 15 fingers, fair in 9 fingers andpoor in 1 finger; the excellent and good rate was 72.2%. There was statistically significant difference in the functional recovery degrees of fingers between two groups (P lt; 0.05). The 2 fingers which had a poor result in 5-FU group and control group were served with tenolysis was performed in 2 cases having poor results after 6 months of operation and had an excellent result at last. Conclusion 5-FU appl ied topically can reduce tendon adhesions after the ruptured tendon repair.

    Release date:2016-09-01 09:14 Export PDF Favorites Scan
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