OBJECTIVE: To observe the functional rehabilitation of injured peripheral nerve with electric acupuncture. METHODS: Sciatic nerve injury model was established by transection of left sciatic nerve in 60 Wistar rats, which were randomly divided into two groups. The experimental group was treated with electroacupuncture, no treatment in the control group. Change of nerve electrophysiological, power of muscle and sciatic functional index (SFI) were observed. RESULTS: Nerve muscle-action potential (MAP) and motor nerve conduction velocity (MNCV) in the experimental group were better than that of the control group (P lt; 0.01). The single muscle twitch and tetanization of gastrocnemius muscle were higher in the experimental group too (P lt; 0.05). SFI were significantly higher in the experimental group (P lt; 0.05). CONCLUSION: Electric acupuncture therapy can improve functional rehabilitation of injured peripheral nerve.
OBJECTIVE: To investigate the effect of electroacupuncture on mRNA expression of NGF and IGF-1 in injured nerve. METHODS: Sciatic nerve injury model was established by transection of right side sciatic nerve in 90 male SD rats, which were randomly divided into two groups. The experimental group was treated with electroacupuncture, no treatment in the control group. The distal part of the injured nerve was harvested after 1, 2, 4, 6 and 10 weeks of operation and stored in the liquid nitrogen. The total RNA was extracted by the TRIzol reagent. Reverse transcriptase-polymerase chain reaction(RT-PCR) was used to detected the mRNA expression of NGF and IGF-1. RESULTS: The mRNA expression of NGF in the experimental group was increased quickly from the second week, and reached to highest level in the fourth week. It was much higher than that of the control group (P lt; 0.05). Then it began to decline in following time and approximately reached to the level of the first week after 10 weeks of operation. The mRNA expression of IGF-1 in the experimental group was remarkably increased in the second and fourth week, and which was much higher than that of the control group respectively(P lt; 0.05). Although the mRNA expression of IGF-1 after 10 weeks of operation in the experimental group was higher than that of the control group, but there was no significant difference between the two groups(P gt; 0.05). There was linear correlation in the fourth week between mRNA expression of NGF and IGF-1 in the experimental group. CONCLUSION: The mRNA expression of NGF and IGF-1 can be elevated in injured nerve at early stage interfered with electroacupuncture.
目的:观察康复训练结合电针疗法综合治疗汶川地震伤员四肢骨折术后关节活动度、肢体肿胀等功能障碍的疗效。方法: 将126例患者分为治疗组63例,采用康复训练结合电针疗法;对照组63例,于术后第2天自行功能锻炼。测量治疗前后关节活动度(ROM)、肢体肿胀消退时间及疼痛减轻程度并据此确定疗效。结果: 用统计学方法处理,说明两组之间ROM、肢体肿胀消退时间及疼痛减轻程度比较均有统计学意义(Plt;0.05)。结论: 采用康复训练结合电针能有效提高地震伤员四肢骨折后关节功能障碍的疗效。
目的 初步观察电针足三里穴对不全肠梗阻大鼠小肠Cajal间质细胞（interstitial cells of Cajal，ICC）数量变化的影响，为进一步探讨电针足三里穴对ICC表型变化的影响奠定基础。方法 采用圈套法造成不全肠梗阻从而建立ICC数目减少的SD大鼠模型。取20只雌性大鼠采用简单随机法均分成正常对照组、不全肠梗阻30d未电针足三里穴组（梗阻组）、不全肠梗组30d电针足三里穴组（足三里组）和不全肠梗阻30d电针阴陵泉穴组（阴陵泉组） 4组。其中足三里组和阴陵泉组分别连续电针足三里穴或阴陵泉穴7d后，取小肠组织采用免疫组化方法以及免疫荧光观察ICC数量的变化。结果 正常对照组、足三里组、梗阻组及阴陵泉组ICC阳性面积分别为（102 051.00±16 969.38） μm2、（92 642.12±14 854.49） μm2、（45 221.33±6 230.20） μm2和（63 136.16±8 863.91） μm2，各组间差异有统计学意义（F=21.240，P＜0.001），其中足三里组的ICC阳性面积较梗阻组高（P＜0.05）。结论 电针足三里穴可使不全肠梗阻大鼠小肠受损的ICC数量得到部分恢复，但其具体机理有待进一步研究。
ObjectiveTo observe the therapeutic effect of electro-acupuncture combined with oral glucosamine hydrochloride capsules in the treatment of knee osteoarthritis, and compare it with the curative effect of the two methods separately. MethodsA total of 168 patients with knee osteoarthritis treated from March 2006 to December 2012 were randomly divided into 3 groups. Electro-acupuncture combined with oral glucosamine hydrochloride capsules were given to the 58 patients in the treatment group, single acupuncture treatment was given to the 57 patients in control group A, and simple oral glucosamine hydrochloride capsules were given to the 53 patients in control group B. The treatment lasted for 6 weeks. Assessment of knee osteoarthritis by Lequesne international standard index number and the total efficiency was carried out before treatment and 3 weeks, 6 weeks and 6 months after treatment, respectively. ResultsThe total effective rate of the treatment group was 91.38%, of control group A was 68.42%, and of control group B was 79.25%. There was a significant difference between the treatment group and control group A, and between the treatment group and control group B (P<0.05). No blood, liver or kidney damage or gastrointestinal adverse reaction occurred among patients in all the three groups. ConclusionThe efficacy of electro-acupuncture plus oral glucosamine hydrochloride capsules is better than simple acupuncture and simple oral glucosamine hydrochloride capsules treatment.
ObjectiveTo systematically review the efficacy and safety of electroacupuncture in animal hyperlipidemia models. MethodsDatabases such as CNKI, WanFang Data, VIP, CBM, PubMed, EMbase and The Cochrane Library (Issue 12, 2013) were searched for studies concerning the efficacy and safety of electroacupuncture in animal hyperlipidemia models up to December 2013. Two reviewers independently screened literature according to the inclusion and exclusion criteria, extracted data and assessed the methodological quality of included studies by using reformative CAMARADES List. Then, meta-analysis was performed by using RevMan 5.2 software. ResultsA total of 4 studies involving 89 rats were included. The average score of CAMARADES List was 4. The results of meta-analysis showed that:there were no significant differences between electroacupuncture group and medication group in reducing the levels of TC (MD=0.06, 95%CI -0.08 to 0.20, P=0.40), TG (MD=-0.01, 95%CI -0.08 to 0.06, P=0.74), LDL-C (MD=0.01, 95%CI -0.04 to 0.03, P=0.65) and increasing the level of HDL-C (MD=-0.00, 95%CI -0.09 to 0.08, P=0.93). ConclusionCurrent evidence shows that there are no differences between electroacupuncture and medication in reducing levels of TC, TG, LDL-C and increasing the level of HDL-C in hyperlipidaemia rats. But due to the limitation of sample size of included studies, more large-scale, high quality studies are needed.
ObjectiveTo investigate the effect of electroacupuncture on the apoptosis of hippocampal neurons in C57BL/6J mice with status epilepticus by observing the changes of hippocampal subtle neuron pathology and apoptosis.MethodsMale C57BL/6J mice were used to prepare epileptic status models of lithium-pilocarpine mice, and then 7-day electroacupuncture stimulation (Baihui, Fengfu) were given to the mice model. Open field experiment and new object recognition experiment were performed to observe the changes of cognitive abilities. The pathological changes of hippocampal neurons were detected by HE staining. Hippocampal apoptosis protein (Caspase-3) and microtubule-associated protein (MAP-2) were detected by immunohistochemistry. Effect of electroacupuncture on apoptosis of hippocampal neurons in C57BL/6J mice with status epilepticus were recorded.Results① Compared with the control group, the vertical movement, modification times, and number of crossings of the model group all decreased significantly (P<0.000 1,P<0.000 1,P<0.000 1), and their cognitive ability decreased significantly (P<0.01). Compared with the model group, vertical movements, modification times, and number of crossings were increased in the electroacupuncture (EA) group (P<0.01,P<0.05,P<0.05), and the cognitive ability of new objects was increased (P<0.01). ② HE staining showed that the model group had significant damage to the hippocampal neurons of mice, and the cells swelled, nuclear collapsed and vacuoles appeared. In the EA group, the injury of hippocampal neurons was alleviated, and cell edema and vacuolization were alleviated. ③ Immunohistochemistry showed that compared with the control group, the IOD of the Caspase-3 positive cells in the hippocampus of the model group increased significantly (P<0.000 1), and the IOD of the MAP-2 positive cells decreased significantly (P<0.01); Compared with the electroacupuncture, the IOD of the Caspase-3 positive cells in the hippocampus of the mice decreased (P<0.05), and the IOD of the MAP-2 positive cells increased (P<0.05).ConclusionsElectroacupuncture can improve the pathological changes of hippocampal neurons in C57BL/6J mice with status epilepticus, promote cytoskeletal repair, reduce neuronal apoptosis in hippocampus, and antagonize the damage of hippocampal neurons induced by status epilepticus.