目的:观察CCl4攻击小鼠致肝纤维化血清IL4、IL6、IL8的动态变化规律及其表达的影响。方法:将小鼠分为4组:雌、雄性小鼠对照组、雌、雄性小鼠体积分数为30%四氯化碳花生油组(皮下注射)。用ELISA及酶标仪方法检测小鼠血清IL4、IL6、IL8的含量及肝脏、脾脏的表达情况。结果: CCl4攻击可使小鼠血清IL6、IL8含量增加,IL4的表达进一步减少。结论:CCl4可能通过攻击小鼠IL4、IL6、IL8的表达参与抗炎反应过程,细胞因子在肝纤维化发病过程中起重要的作用。检测血清IL4、IL6、IL8浓度的变化可作为了解其与肝纤维化的关系。
Objective To observe the effects of immunologic cytokines or anti-angiogenesis gene transfer mediated by electroporation for choroidal melanoma cells.Methods The human embryo kidney cells and malignant choroidal melanoma cells were transfected with plasmids pNGVL-mIL2, pNGVL-mIL12, pCI-sFLK-1, pCR3.1-antiVEGF121,pCI-ExTek. Then the expression of mIL2, mIL12, sFLK-1, VEGF and ExTek were detected by enzymelinked immunosorbentassay (ELISA) and Western blot. Nude mice models of malignant choroidal melanoma were established and they were divided into four groups randomly. Each group was treated with 30 mu;l of 0.9% NaCl, 30 mu;g pNGVL, 30 mu;g antiVEGF121+sFLK-1+ExTek and 30 mu;g mIL2+mIL12 respectively by electroporation. Seven，14, 21, 28, 35 and 42 days after treatment, the tumor volumes were measured to calculate the tumor inhibition rate. Results ELISA and Western blot showed that mIL2，mIL12，sFLK-1 and ExTek were expressed after electroporation，VEGF expression was decreased remarkably. After treatment，the tumors of mIL2+mIL12 group were greatly inhibited with a tumor inhibition rate of 97.33%，while the tumors of antiVEGF121+sFLK-1+ExTek and pNGVL group were partially inhibited with tumor inhibition rates of 53.33% and 36.33% respectively.Conclusions Immunologic cytokines transfer mediated by electroporation can inhibit the growth of melanoma，but anti-angiogenesis only have a mild effects.
Objective To investigate production of interleukine 6 (IL-6)and interleukine 8 (IL-8)by retinal pigment epithelial (RPE)cells and its inhibition by interleukine 1 receptor antigonist (IL-1ra).Methods cultured human RPE cells was treated with interleukine 1beta;(IL-1beta;,10ng/ml)and/orIL-1ra(IL-1ra,1、10、100 ng/ml).IL-6 and IL-8 mRNA and protein expression were detected by ELISA, immunohistochemistry and in situ hybridization (ISH)assay. Results IL-6 and IL-8 in conditioned media of RPE cells in controls was 2000 pg/ml and 5000 pg/ml respectively after stimulation of IL-1beta; FOR 8h.IL-1ra(100ng/ml)significantly inhibited IL-6(300 pg/ml,t=8.011,P＜0.01)and IL-8(450 pg/ml,t=11.446,P＜0.01) production compared with the controls.In situ hybridization displayed that there was a marked reduction in mRNA expression of IL-6 and IL-8 in IL-1ra treated group compared with controls.Conclusion The production of IL-6 and IL-8 can be significantly reduced by IL-1ra in cultured human RPE cells after stimulation of IL-1beta;
ObjectiveTo analyze the expression of VEGF, IL-33 and NO concentration after laser photocoagulation and subthreshold micropulse laser photocoagulation conventional in proliferative diabetic retinopathy (PDR) patients.MethodsA case control study. The clinical data of 39 patients of PDR and 11 patients of idiopathic macular pucker (IMP) from Department of Ophthalmology, Central Theater General Hospital during November 2015 were collected in this study. PDR patients were assigned randomly into three groups. Fifteen PDR patients with 15 eyes were treated with conventional laser as group A. Thirteen PDR patients with 13 eyes were treated with subthreshold micropulse laser as group B. Eleven PDR patients with 11 eyes without any laser therapy were grouped as C. Eleven IMP patients were grouped as D. There was no difference of age (F=0.53, P=0.23), gender ratio (χ2=0.55, P=0.91), body mass index (F=2.62, P=0.07), duration diabetes (F=0.29, P=0.75), glycoslated hemglobin (F=1.72, P=0.19) in four groups. All PDR patients were examined with FFA. Total protein was quantified by a bicinchoninic acid assay kit. Levels of VEGF, IL-33, NO were determined using enzyme-linked immunosorbent assay kits.ResultsThere was no difference of total protein in four groups (F=1.78, P=0.17). Group C had a higher VEGF level than group A and B (F=7.84, P=0.002). Group A had a higher IL-33 level than group C (t=4.15, P=0.02). There was no difference of IL-33 level in group B and C (t=1.34, P=0.20). Group D had a lower NO level than group A, B, C (F=38.42, P＜0.001). There was no difference of NO level in group A, B and C (F=3.29, P=0.06).ConclusionsBoth conventional laser photocoagulation and subthreshold micropulse laser photocoagulation can decrease vitreous VEGF level and subthreshold micropulse laser photocoagulation can induce less IL-33 level.