目的 探讨基质金属蛋白酶3（MMP-3）及其抑制因子2（TIMP-2）在肺鳞癌中的表达及临床意义。 方法 采用免疫组织化学方法（SP法），分别检测96例肺鳞癌组织和20例正常支气管黏膜上皮组织中MMP-3及TIMP-2的蛋白表达。 结果 MMP-3及TIMP-2在96例肺鳞癌组织中的阳性表达率（分别为81.3%、51.0%）明显高于正常支气管黏膜上皮组织（分别为25.0%、15.0%）；与无淋巴结转移的肺鳞癌组织相比，伴淋巴结转移的肺鳞癌组织中MMP-3、TIMP-2的阳性表达率明显升高，且差异均有显著的统计学意义（P＜0.01）。MMP-3的阳性表达随临床分期增加而有增高趋势，且差异有非常显著的统计学意义(P＜0.01)；TIMP-2在中晚期肺鳞癌组织（Ⅲ）的阳性表达率较早期肺鳞癌组织（Ⅰ+Ⅱ）有下降趋势，但差异无统计学意义（P＞0.05），二者之间表达也无明显相关性（r=−0.095，P＞0.05）。与无淋巴结转移肺鳞癌组织比较，有淋巴结转移肺鳞癌组织中，MMP-3的表达增高，TIMP-2的表达明显下降，二者之间表达呈负相关（r=−0.294，P＜0.05）。 结论 MMP-3与TIMP-2的过度表达与肺鳞癌的发展、淋巴结转移及临床TNM分期关系密切。MMP-3和TIMP-2在肺鳞癌组织中的表达情况，对预测肺鳞癌的浸润、转移，判断复发危险等方面具有重要意义，并有望成为判断肺鳞癌预后的生物学标志物。
Objective To explore the protein expressions of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1)， and to investigate their relationships between their serum concentration before operation and the infiltration and metastasis of thyroid carcinoma. Methods The protein expressions of MMP-9 and TIMP-1 in 32 cases of thyroid carcinomas, 23 cases of adjacent tissues and 30 cases of benign hyperplastic lesions were measured by using immunohistochemistry. The preoperative serum concentrations of MMP-9 and TIMP-1 in 21 cases of thyroid carcinomas and 19 cases of benign hyperplastic lesions were determined by enzyme-linked immunosorbent assay. Results The positive expression rates of MMP-9 and TIMP-1 in tumor tissues were significantly higher （75.0%,56.3%）than those in adjacent tissues and benign hyperplastic lesions （30.4%, 21.7%; 26.7%, 23.3%) P＜0.05. There were correlations between the expressions of MMP-9 and TIMP-1 and the local infiltrative degrees, lymph node metastasis and TNM stage （P＜0.05）. There was a negative correlation between the expression of MMP-9 and the expression of TIMP-1 （r=-0.509， P=0.003）. The concentration of MMP-9 in serum of thyroid carcinoma patients was (122.60±36.20) ng/ml, whereas TIMP-1 was (59.44±38.65) ng/ml, both of which were significantly higher compared to those of benign group (P＜0.05). In addition, there was a positive correlation between the expressions of MMP-9/TIMP-1 in the carcinoma tissues and their concentrations in serum （P＜0.05）.Conclusion To detection the expressions of MMP-9 and TIMP-1 in the lesion and their concentrations in the serum may not only contribute to the differential diagnosis of thyroid tumors, but may also help to predict the prognosis of the carcinoma.