Objective To probe into disorder of plasma lipids and apolipoproteins in patients with gallstone,and their position and function in formation of gallstone. MethodsConcentration of plasma lipids and apolipoproteins in 94 healthy subjects and 161 patients with gallstones was investigated. ResultsThe gallstone group had a higher serum mean concentration of TG,Apo CⅡ,Apo CⅢ, and had a lower serum mean concentration of TC，HDLc，HDL2c，HDL3c and LDLc as compared with the control group (P＜0.01 or P＜0.05). Conclusion Higher serum mean concentration of TG，Apo CⅡ，Apo CⅢ, and lower serum mean concentration of TC, HDLc, HDL2c, HDL3c and LDLc, are characteristic of lipids metabolism and important cause of formation of gallstone.
目的探讨胆囊结石病引起胆道内瘘的原因，提高其诊断和治疗水平。方法收集我院1990～1999年收治的胆道内瘘患者46例，并对其病因、诊断与治疗进行分析。结果胆囊十二指肠瘘18例（39.1%），胆囊结肠瘘8例（17.4%），胆囊与胃、空肠内瘘各1例（4.3%）； 胆囊胆管瘘18例（39.1%）。术后出现并发症4例（8.7%），死亡1例（2.2%），治愈率为97.8%。结论重视本病的临床表现及术前、术中检查，选择恰当手术方式，可减少死亡与并发症的发生。
Objectiveofthisstudyistoprognosethepossibilityofdevelopinggallstoneinsubjectswiththedyslipidemiaandobesity.Themultivariablelogisticregressionmodelwasusedtoevaluatetheoddsratio(OR)ofthedyslipidemiaandobesitytoinducetheformationofgallstone.ORgt;1indicatesdangerousfactor,ORlt;1protectivefactor,andOR=1nosignificance.Theresultsshowedthatiftriglyceride(TG)andverylowdensitylipoproteincholesterol(VLDLC)increasedanaveragelevelofnormalrespectively,andtherewouldbeORofTG2.43(Plt;0.05)andORofVLDLC6.09(Plt;0.05),thehighlevelsofTGandVLDLCwerethefactorsoflithogenesis.Highdensitylipoproteincholesterols(HDL1C,HDL2C,HDL3C),withORlessthanone,werethefactorsofprotectingagainsttheformationofgallstone.ORoflowdensitylipoproteincholesterol(LDLC)andORoftotalcholesterol(TC)werealsolessthanone,butpresentresearchindicatedthattheymaybeawayoflipidmetabolismnottobeaprotectivefactor.ORofBMIinmalesubjectswas1.16(Pgt;0.05),andinfemale1.38(Plt;0.05).Thesesuggestthatbothcorrectionofthemetabolismofdyslipidemiaandreductionofbodyweightareimportanttodecreasethemorbidityofcholecystolithiasis.
Objective To investigate the frequency of variant at XmnⅠ, MspⅠ sites of apolipoprotein (Apo) AⅠ-CⅢ-AⅣ gene cluster and its relation to cholesterol cholecystolithiasis in Chinese population. Methods The restriction fragment length polymorphisms (RFLP) at Xmn Ⅰ, MspⅠ sites of ApoAⅠ-CⅢ-AⅣ gene cluster was studied by using polymerase chain reaction (PCR) in healthy subjects (control group) and patients with gallstones(gallstone group) from a population of Chinese with the Han nationality in Sichuan Province. Results The X1, M1 alleles were the major alleles in both gallstone group and control group, and X1X1, M1M homozygous genotypes were the most frequent ones. The frequencies of X1X2, X2X2 and X2 alleles in female patients of the gallstone group were significantly higher than those in female of control group (P＜0.05), and the frequencies of X1X1 and X1 alleles in control group were significantly higher than those in gallstone group (P＜0.05). The frequencies of M1M1 in male patients of the gallstone group were significantly lower than those in male of control group (P＜0.05), whereas, the frequencies of M1M2 were significantly higher in gallstone group (P＜0.05). Conclusion The polymorphism of XmnⅠ RFLP of ApoAⅠ-CⅢ-AⅣ gene cluster in female and the polymorphism of MspⅠ RFLP of ApoAⅠ-CⅢ-AⅣ gene cluster in male may be associated with cholesterol cholecystolithiasis in Chinese population.
ObjectiveTo explore technical essentials and safety of laparoscopic cholecystectomy (LC) guided by gallbladder ampulla localization on an imaginary clock for cholecystitis.MethodsA retrospective study of 8 707 continuous patients with mild cholecystitis who underwent LC from July 1998 to February 2018 at a single institution was conducted. Among them, 3 168 patients were treated by the traditional LC from July 1998 to February 2007 (a traditional LC group), 5 539 patients were treated by the LC with the guidance of the gallbladder ampulla localization on an imaginary clock from March 2007 to February 2018 (a gallbladder ampulla localization group). The conversion to open surgery, bile duct injury, return to the operating room due to postoperative massive abdominal bleeding, bile leakage without bile duct injury, operative time, intraoperative blood loss, and postoperative hospital stays were compared between the traditional LC group and the gallbladder ampulla localization group.ResultsThere were no significant differences in the gender, age, course of disease, and type of cholecystitis between these two groups (P>0.050). The rates of conversion to open surgery, bile duct injury, return to the operating room due to postoperative massive abdominal bleeding, bile leakage without bile duct injury and the operative time, intraoperative blood loss and postoperative hospital stays in the traditional LC group were 3.00% (95/3 168), 0.13% (4/3 168), 0.09% (3/3 168), 0.03% (1/3 168), (43.6±12.6) min, (18.7±3.3) mL, (3.6±2.7) d, respectively, which in the gallbladder ampulla localization group were 0 (0/5 539), 0 (0/5 539), 0 (0/5 539), 0 (0/5 539), (32.2±10.5) min, (12.4±3.5) mL, (3.5±2.8) d, respectively. The differences of conversion to open surgery, bile duct injury, return to the operating room due to postoperative massive abdominal bleeding rates, and the operative time and intraoperative blood loss were statistically significant between these two groups (P<0.050). The differences of the bile leakage without bile duct injury rate and postoperative hospital stays were not statistically significant between the two groups (P>0.050).ConclusionThis study shows that gallbladder ampulla localization on an imaginary clock is useful for ductal identification so as to reduce bile duct injury and improve safety of LC in case of no conversion to open surgery.