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find Keyword "荧光定量聚合酶链式反应" 2 results
  • 荧光定量聚合酶链反应检测法诊断新生儿乙型肝炎病毒感染的卫生经济学评价

    目的 探讨采用荧光定量聚合酶链反应(FQ-PCR)检测法诊断新生儿乙型肝炎病毒(HBV)感染的经济学成本。 方法 对2010年3月-2010年7月间202例日龄在28 d以内的新生儿采用酶联免疫吸附法检测HBV血清学标志物,对于HBV血清学标志物中除乙型肝炎表面抗原外其余任何一项或一项以上阳性的新生儿采用FQ-PCR检测其血清HBV-DNA的含量,并分析确诊1例HBV感染病例的费用。 结果 血清HBV-DNA水平与HBV标志物表现模式有关,乙型肝炎e抗原(HBeAg)阳性的新生儿,FQ-PCR阳性率为3/16 (18.8%)。HBeAg阴性之新生儿,FQ-PCR阳性率为1/186(5.4‰)。HBeAg阳性病例为进一步明确乙型肝炎病毒含量而进行FQ-PCR检测所产生的费用为587元/例,HBeAg阴性者为20 460元/例。 结论 FQ-PCR确诊HBeAg阳性的新生儿为HBV感染的费用-效益比远高于HBeAg阴性的新生儿,对于HBeAg阴性的新生儿该法不适合大规模的流行病学诊断性研究。

    Release date:2016-09-07 02:34 Export PDF Favorites Scan
  • Construction of real-time polymerase chain reaction detection for infection-related cytokines of tree shrew

    Tree shrew is a novel and high-quality experimental animal model. In this study, the real-time polymerase chain reaction methods were established to detect infection-related cytokines interleukin-6 (IL-6), IL-8, IL-10, IL-17A, interferon-γ (IFN-γ) and housekeeping gene glyceraldehyde-phosphate dehydrogenase (GAPDH) of tree shrew. The results indicated that the establised methods had good specificity. The high point of the linear range of these reagents reached 1 × 1010 copies, and the low points ranged from 10 copies (IL-6, IL-17A), 100 copies (IL-10, GAPDH) to 1 000 copies (IL-8, IFN-γ). In this interval, the linear correlation coefficient R2 of each reagent was greater than 0.99. The lowest detectable values of IL-6, IL-8, IL-10, IL-17A, IFN-γ and GAPDH were 8, 8, 4, 8, 128 and 4 copies, respectively. The results showed that the established detection methods had good specificity, sensitivity and wide linear range. The methods were suitable for detection of multiple concentration range samples, and could be used for the subsequent studies of tree shrew cytokines.

    Release date:2019-06-17 04:41 Export PDF Favorites Scan
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