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find Keyword "荧光染料" 2 results
  • Iris pigment epithelial cells of rabbits stained by carboxyfluorescein diacetate succinimidyl ester in vitro

    Objective lt;brgt;To investigate the feasibility of labeling iris pigment epithelial(IPE)cells of rabbits with 5(and 6)carboxyfluorescein diacetate succinimidyl ester(CFSE). lt;brgt; lt;brgt;Methods lt;brgt;Enzyme-assisted microdissection was used to isolate the cultured rabbitprime;s IPE cells.The third or forth subcultured IPE cells were incubated with 2.5,5,10,20,and 40 mu;mol/L of CFSE for 1,5,and10min respectively.The fluorescence intensity was detected by flow cytometry,and the leakage of CFSE and its dyeing were observed by fluorescence antibody labeling. lt;brgt;Results lt;brgt;Incubation with 20 mu;mol/L CFSE under 37℃for1minute was the most optimal condition for IPE cells labeling.The coloration of IPE cells stained by CFSE lasted 4 weeks.There was no leakage of dye from labeled rabbit IPE cells to non-labeled human IPE cells in mixed culture process. lt;brgt; lt;brgt;Conclusion lt;brgt;With the advantages of high rate of dyeing,long time of tracing,safety and convenience,CFSE can be used as a new method to label the rabbitprime;s IPE cells. lt;brgt; lt;brgt;(Chin J Ocul Fundus Dis, 2006, 22: 261-264)

    Release date:2016-09-02 05:51 Export PDF Favorites Scan
  • 荧光金逆行标记大鼠视网膜节细胞

    Release date:2016-09-02 06:07 Export PDF Favorites Scan
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