Objective o explore the effect and mechanisms of transmembrane 4 super family (TM4SF) in digestive system cancer. Methods Articles were reviewed to discuss the biological characteristics of TM4SF in digestive system cancer. Results TM4SF played an important role in migration and invasion of digestive system cancer, including pancreatic cancer, gastric cancer, colorectal cancer, hepatic cancer, esophageal cancer, and so on. TM4SF modulated the cell biological activities by microdomains which were fixed on cell membrane, such as adhesion, migration, invasion, and proliferation. Conclusion TM4SF may be used to predict the metastasis and prognosis of digestive system cancer and may be the targets of therapy of it in the future.
ObjectiveTo explore the value of duodenum-preserving pancreatic head resection for benign lesions in pancreatic head. MethodsClinical data of 14 patients with benign lesions in pancreatic head who were underwent duodenum-preserving pancreatic head resection from Jan. 1995 to Dec. 2012 were retrospectively analyzed, to explore the value of the surgery. ResultsAll surgeries of the 14 patients were success. The operation time were 4.0-6.5 hours (5.2 hours on average), the intraoperative blood loss were 100-1 000 mL (450 mL on average). Postoperative complications happened in 3 patients (21.4%), including pancreatic fistula in 2 patients and biliary fistula in 1 patient, which were cured with non-surgical treatment. No deaths happened during surgery and hospitalization. Of the 14 patients, 13 patients were followed-up for 6 months to 2 years with no recurrence, diabetes, postoperative gastric emptying disorders, and other long-term complications happened. ConclusionDuodenum-preserving pancreatic head resection is the safe and effective treatment of benign lesions in the head of pancreas.
ObjectiveTo evaluate the clinical manifestation, diagnosis, and treatment of intrahepatic biliary cystadenoma (IBCA). MethodsThe clinical date of 12 patients with IBCA from January 2004 to December 2013 in our hospital were retrospectively analyzed. ResultsOf the total 10 patients were female and 2 were male. The average age was 49.0 years old (16-77 49.0 years old). The course of disease were 1 month-3years (average:7 months). The clinical symptoms were right upper abdominal fullness with a sense of pain in 6 patients, right upper abdominal mass in 1 case, jaundice in 1 case, and no symptoms in 4 cases. Enhanced CT examination showed that the tumor complete capsule and there were internal septations in 11 cases, the density of internal septations was different. There was mild enhancement of cyst wall and internal septations in 9 cases. All of 12 cases received surgical resection. Only 1 case showed recurrence in 2 years after operation, the remaining 11 patients had no recurrence. ConclusionsIBCA often occurs in middle-aged women. The main clinical symptoms are abdominal fullness with a sense of pain, right upper abdominal mass or jaundice. Enhanced CT is the main method of diagnosis before operation. Radical resection of IBCA is the best treatment, and can prevent recurrence effectively.
Objective To assess clinical outcomes of hand assisted laparoscopic right hemicolectomy (HALC) and standard laparoscopic right hemicolectomy (SLC). Methods The databases of Wanfang, CNKI, VIP, CBM, PubMed, Embase, and Cochrane Central Register of Controlled Trials were electronically searched. The relevant literatures were selected according to the inclusion and exclusion criteria. The Cochrane collaboration tool for assessing risk of bias was used to assess the quality of randomized controlled trials and the Newcastle-Ottawa Scale was used to assess non-randomized comparative studies. Meta-analysis was performed by using RevMan 5.3 software. Results A total of 9 studies were included and involved 976 patients (480 patients in the HALC group and 496 patients in the SLC group). The results of meta-analysis showed that the HALC group was favor of shorter operative time as compared with the SLC group (P<0.05), but the length of incision and hospital stay were longer in the HALC group (P<0.05). There were no statistically significant differences between these two groups regarding as the conversion rate, time to return of bowel function, feeding time, reoperative rate during hospitalization, postoperative complications rate, and harvested lymph node number (P>0.05). As for the follow-up results, the 3-year survival rate was about 90%, and 5-year survival rate was about 80%, and there were no statistical differences in terms of recurrence rate and mortality between the HALC group and the SLC group (P>0.05). Conclusions Both HALC and SLC could achieve satisfactory minimal invasive outcomes and oncologic radical effects, and HALC has an advantage of shorter operative time, yet length of incision and hospital stay are longer than SLC. Therefore, HALC could be considered as an alternative to minimal invasive right hemicolectomy.
Cardiac three-dimensional electrophysiological labeling technology is the prerequisite and foundation of atrial fibrillation (AF) ablation surgery, and invasive labeling is the current clinical method, but there are many shortcomings such as large trauma, long procedure duration, and low success rate. In recent years, because of its non-invasive and convenient characteristics, ex vivo labeling has become a new direction for the development of electrophysiological labeling technology. With the rapid development of computer hardware and software as well as the accumulation of clinical database, the application of deep learning technology in electrocardiogram (ECG) data is becoming more extensive and has made great progress, which provides new ideas for the research of ex vivo cardiac mapping and intelligent labeling of AF substrates. This paper reviewed the research progress in the fields of ECG forward problem, ECG inverse problem, and the application of deep learning in AF labeling, discussed the problems of ex vivo intelligent labeling of AF substrates and the possible approaches to solve them, prospected the challenges and future directions for ex vivo cardiac electrophysiology labeling.
【 Abstract 】 Objective Overexpressions of epidermal growth factor (EGF) and EGF receptor have been associated with progression and invasive phenotype of pancreatic cancer. However, the underlying molecular mechanism by which EGF worked in pancreatic cancer cells has not been completely understood. In this study, effect of EGF on the invasion and metastasis of pancreatic cancer cells and its regulatory mechanism were investigated. Methods The effects of EGF on the proliferation, adhesion and invasion of pancreatic cancer cells were detected by WST-1 proliferation assay, adhesion assay and invasive assay, respectively. The activity and expression of MMP-2 and MMP-9 were examined by zymography, Western blot and RT-PCR, respectively. The activity of NF- κ B was examined by EMSA. Results EGF could significantly promote the invasiveness of pancreatic cancer cells but did not affect cell proliferation or adhesion. The expressions of NF- κ B and MMP-9 were significantly increased by EGF, but EGF did not affect the activity and expression of MMP-2. Furthermore, EGF stimulated the NF- κ B binding activity. Pretreatment with NF- κ B inhibitors, pyrrolidine dithiocarbamate (PDTC), could significantly inhibit the activity of NF- κ B induced by EGF. Meanwhile, the EGF-induced expression and activity of MMP-9, as well as cell invasiveness were also inhibited by NF- κ B inhibitor. Conclusion EGF could increase the expression and promote the invasiveness of MMP-9 via the activation of NF- κ B in pancreatic cancer cells, which implies that NF- κ B inhibitant, such as PDTC, may diminish the invasiveness of pancreatic cancer cells.
【Abstract】 Objective To explore the features of Ki-ras mutations at codon 12 in Chinese patients of pancreatic cancer and to compare these features with those in Western countries. Methods Fifty-nine samples were collected during operations for pancreatic adenocarcinoma in our hospital from December 1989 to November 1997. The patients, age ranged from 30 to 73 years 〔(55.5±10.4) years〕,with 38 males and 21 female. TNM staging of the patients: stage Ⅰ(n=4); stage Ⅱ(n=8), stage Ⅲ(n=42),stage Ⅳ(n=5). PCR was used to amplify target gene and Dot blot hybridization for detecting Ki-ras mutations at codon 12 was performed in fifty-nine specimens of Chinese pancreatic cancer. The data of Ki-ras mutations at codon 12 from Western countries were gotten by Medline system. Results Ki-ras mutation at codon 12 was detected in 76.3% of the patients in this group. The frequency of double mutation of Ki-ras at codon 12 in this group (15.6%) was highest than that in western countries. Our results were compared with those reported in Western countries. The results suggested that there were the significant differences in the substitution of Ki-ras mutations at codon 12 and in the ratio of transition to transversion in pancreatic cancer among various countries. Conclusion Ki-ras mutations at codon 12 is frequent in Chinese pancreatic cancer, and a gene component to pancreatic cancer may be different among various countries. In addition, the effect of Ki-ras mutations at codon 12 on prognosis of patients with pancreatic cancer is different in various countries.
目的总结胰腺血管活性肠肽瘤（VIPoma）的临床特点并探讨其诊治方法。 方法回顾性分析1991年11月至2010年2月期间我院收治的3例VIPoma患者的临床资料。 结果3例均有周期性发作水样泻、低钾血症和低或无胃酸。有2例血浆血管活性肠肽值显著升高。B超及CT发现3例肿瘤均位于胰腺内，其中1例肝转移，1例怀疑肝转移。3例均行手术治疗。1例肿瘤位于胰头部行胰十二指肠切除术，随访3年无肿瘤复发；1例肿瘤位于胰头颈部及肝脏多发转移灶，行胰腺头颈部切除及肝脏转移结节摘除术，术后4个月腹泻症状复发，证实肝脏多发转移灶，又先后3次手术，于第1次手术后3.5年死于电解质紊乱；另1例术中发现肿瘤位于胰头部无法切除，肝脏多发转移灶，摘除1枚肝脏结节病理确认为转移癌，术后间断应用奥曲肽，术后2.5年仍在随访中。 结论VIPoma首选的治疗方法是手术切除肿瘤。恶性肿瘤即使无法根治手术，也应该姑息性切除和间断应用奥曲肽，以改善患者生活质量。