Objective To analyze the correlation and difference of axial length (AL) measurements by comparing magnetic resonance imaging (MRI) of different pulse sequence with IOL Master of normal eyes. Methods The 67 emmetropic volunteers were selected by the slit lamp microscope, direct ophthalmoscope and refractive examination in this study. The patients included 30 males and 37 females. The patientsprime; ages ranged from eight to 21 years, with a mean age of 14.9 years. Fast spin echo (FSE) T1WI, FSE T2WI, fluid attenuated inversion recovery (FLAIR) T2WI, FSPGR T1WI were performed. The axial (AX) and oblique sagittal (OS) sections were taken and the AL from MRI section with different sequences were measured. The AL taken by IOL-Master was used as standard. The difference and correlation between measurements of two methods were analyzed. Results The AL of AX FSE T1WI, AX FSE T2WI, AX FLAIR T2WI, AX FSPGR T1WI, OS FSE T1WI, OS FSE T2WI, OS FLAIRT2WI and OS FSPGR T1WI were (24.14plusmn;0.81), (24.26plusmn;0.81), (23.87plusmn;0.79), (24.11plusmn;0.82), (24.08plusmn;0.86), (24.22plusmn;0.81), (23.84plusmn;0.79) and (24.03plusmn;0.81) mm, respectively. The AL of IOLMaster was (23.91plusmn;0.80) mm. The differences of AL between IOL-Master and various MRI sequences were statistically significant (t=－13.54,－23.20, 4.08, －15.55, －8.00, －23.22, 5.25, －10.62;P＜0.01). There were excellent correlations between IOL-Master and various MRI sequences (r=0.97, 0.96, 0.98, 0.97, 0.96, 0.80, 0.98, 0.97;P＜0.01). Conclusion There are differences and significant correlations between MRI sequences and IOL-Master.