Objective To observe the degradation regulation of ubiquitinproteasome inhibitor nuclear factor kappa;B（NF-kappa;B）and its inhibitory signal protein Ikappa;B kinase in earlier period diabetic retinopathy（DR）,and the effects on retinal ganglion cells (RGC) apoptosis.Methods Forty healthy adult Wistar rats were randomly divided into control (group A）,DR（group B）,DR+lowconcentration MG132 treated （group C）and DR+high concentration MG132 treated（group D）groups,10 rats in each group.After 6 and 8 weeks,the results of body masses and fasting blood glucose (FBG) were detected,the expression of NF-kappa;B and Ikappa;B were observed by immunohistochemistry respectively.RGC apoptosis was assessed by the terminal deoxynucleotidyl transferase mediated dUTP-biotin nick-end labelling (TUNEL) method.Results The expression of NF-kappa;B was upregulated in group B compared with group A,its expression decreased in group D compared with group B; but the expression of Ikappa;B was contrary to NF-kappa;B; RGC apoptosis was followed a similar pattern with the expression of NF-kappa;B; the differences among them were statistically significant (P＜0.01).Compared the expression of NF-kappa;B，Ikappa;B and RGC apoptosis in group C and D, there were no statistically significant differences(P＞0.05).Conclusion Ubiquitin-proteasome inhibitor MG132 can block the activation of NF-kappa;B,inhibit ubiquitination of Ikappa;B degradation and RGC apoptosis.
Objective To investigate the clinical effectiveness of focused ultrasound therapy for non-neoplastic epithelial disorders of vulva. Methods Totally 90 patients with pathologically confirmed non-neoplastic epithelial disorders of vulva were enrolled in the study and randomized to receive ultrasound therapy（60 patients）or spectrum therapy (30 patients). The short-term effectiveness and recurrence rate were assessed in both groups at 6 months after treatment. Results In the ultrasound group, 29 cases were cured, 25 were significantly improved, 6 were improved and 5 experienced recurrence. Whereas, in the control group, 1 case was cured, 25 were significantly improved, 4 remained unchanged and 21 experienced recurrence. The short-term effectiveness of ultrasound therapy was superior to that of spectrum therapy (Z=-6.191, P=0.000). And ultrasound therapy was associated with a significantly lower rate of recurrence (χ2=-37.02, P=0.000). Conclusions The focused ultrasound therapy appears to be an effective approach in the treatment of non-neoplastic epithelial disorders of vulva.