Search

Sequential Study of the Complement Activation and Cell Apoptosis in Perihematoma tissue in rats

摘要:目的:动态观察大鼠脑出血后血肿周围组织补体激活与细胞凋亡的规律。方法:用胶原酶注入到大鼠尾状核的方法制作脑出血模型。将大鼠分为脑出血、假手术组、正常组3组。采用苏木素伊红(HE) 染色、免疫组织化学染色及原位末端脱氧核苷酸转移酶介导的dUTP 缺口末端标记法(TUNEL)分别观察各组在脑出血后第6 h、12 h、24 h、48 h、72 h、5 d、7 d时血肿周围补体C3、促凋亡基因(Bax)、抑凋亡基因(Bclxl)及TUNEL的表达。结果补体C3的表达峰值在24~48 h;TUNEL、Bax蛋白表达术后12h增加,48~72 h达高峰,而Bclxl蛋白表达高峰在48h。结论:大鼠脑出血后血肿周围组织补体C3的表达增加与细胞凋亡的演变趋势一致,C3与凋亡有相关。Abstract: Objective: To study the complement activation and apoptosis regular genes changes in the tissues of the perihematoma of intracerebral hemorrhage (ICH) in rats. Methods: Intracerebral hemorrhage was induced in rats by injection of bacterial collagenase into the caudate nucleus. Histopathological changes were studied in 6 h,12 h, 24 h, 2 d, 3 d, 5 d, 7 d after the injection. The immunohistochemistry and TUNEL analysis were performed. The expression of complement factor C3, the TUNELpositive cells, the proapoptotic gene expression (Bax) and the antiapoptotic gene (Bclxl) were examined. Results: The expression of C3 increased to its maximum between 2448 h. The TUNELpositive cells and Bax protein expression increased gradually and reached the peak level between 4872 h. The Bclxl protein reached the peak level at 48 h. The correlation analysis showed that the quantity of C3 was positively related to that of the TUNELpositive cells, but the bax protein was not related to Bclxl protein. Conclusion: The expression of complement factor C3 may contributes to the nerve injury after cerebral hemorrhage and relate to the apotosis in the tissues surrounding the hametoma in rats.

肝叶切除后胆汁免疫球蛋白和补体变化的临床意义

CLINICAL STUDY ON SERUM COMPLEMENT-3 AND PLASMA FIBRONECTIN OF PATIENTS WITH OBSTRUCTIVE JAUNDICE PRE- AND POST-OPERATION

Thirty patients with obstructive jaundice were investigated for serum complement-3 (C3) and plasma fibronectin (FN).The levels of C3 and FN of the juandiced patients were higher than that of thirty patients without obstructive jaundice (Ｐ＜0.01). As compared to pre-operation, the level of C3 of the jaundiced patients decreased obviously within two weeks after operation(Ｐ＜0.01), and recovered in the third week after operation. The level of FN of the juandice patients decreased evidently within one week(Ｐ＜0.01), and recovered in the second week after operation. However, the levels of C3 and FN of the patients without obstructive jaundice changed slightly after operation (Ｐ＜0.05). The high levels of C3 and FN of jaundiced patients may be relative to the latent infection. Consumption and immune imparing may be the reasons of C3 and FN to decrease.

The Role of theComplement Receptor 1 and 3 on Neutrophils in Distinguishing Bacterial Infection in Patients with Acute Exacerbation of Chronic Obstructive Pulmonary Disease

Objective To study the role of the complement receptor 1 and 3 ( CR1 and CR3) on neutrophils in distinguishing bacterial infection in patients with acute exacerbation of chronic obstructive pulmonary disease ( AECOPD) . Methods 40 patients with AECOPD were divided into two groups according to the detection of bacteria in subairway. 20 patients with stable COPD and 20 healthy subjects with no history of smoking were also included. According to Anthonisen criteria, 40 AECOPD patients weredivided into type Ⅰ( 11 cases) , type Ⅱ ( 12 cases) , and type Ⅲ( 17 cases) . The levels of CR1 and CR3 in blood were measured by flow cytometry. Results In AECOPD patients, 25 cases were detected bacteria,and 15 cases were not detected bacteria. The level of CR1 and CR3 were highest in the bacterial infection group than other groups, and highest in type Ⅰ AECOPD patients than other types. ROC analysis showed that CR1 and CR3 had good diagnostic value in bacterial infection in AECOPD, with optimal cutoff values of 11 and 52, respectively. Conclusion CR1 and CR3 may be good index of distinguishing bacterial infection in AECOPD.

Preserving Effect on Myocardium in Porcine Aortic Valve Replacement by Minimal Extracorporeal Circulation

Objective To observe preserving effect on myocytes in porcine aortic valve replacement with minimal extracorporeal circulation (MECC). Methods 7 pigs were collected as experimental animals and undertook aortic valve replacement with MECC. Morphological and immunofluorescence intensity changes of right atrial and left ventricular tissues were observed. Results HE staining showed that there were not significant changes and edema or injury of myocytes of right atriums and left ventricles between preoperation and postoperation. Immunofluorescence staining showed complement C3b/c in right atrial myocardial tissues after the operation were a little ber, and innate antibody IgG were a little ber in left ventricular myocardial tissues but similarly weak in right atrial myocardial tissues pre- and post-operation. There was not significant changes in HSPG staining in pre-and post-operative right atrial myocardial tissues, but HSPG were obviously weaker in left ventricular myocardial tissues after the operation. Conclusion MECC is effective on support of porcine aorta valve replacement.

补体系统在老年性黄斑变性发病机制中的作用

补体系统是机体固有免疫的重要组成部分，包含分布于血浆中或细胞表面的40多种物质，可通过经典途径、凝集素途径或替代途径激活。补体系统通过炎症免疫、血管内皮生长因子表达上调、氧化应激等途径参与和影响老年性黄斑变性（AMD）的发生发展过程；其相关分子的基因多态性与AMD的易感性息息相关；靶向补体系统的治疗对AMD也有一定的疗效。补体系统与AMD相互关系的研究将有助于进一步阐明AMD的发病机制并为其治疗提供新的方向。

补体在渗出型老年性黄斑变性中的作用研究进展

补体系统是机体免疫系统的重要组成部分，广泛参与机体抗微生物防御反应，清除凋亡细胞和免疫复合物以及维持组织稳态，进行免疫调节。补体系统与老年性黄斑变性(AMD)发生也密切相关。在玻璃膜疣中，含有多种选择激活途径中的蛋白；补体成分基因变异也使AMD的发病风险大大增加。补体系统多种分子与AMD CNV形成关系密切，针对补体系统异常激活的治疗可能为渗出型AMD的治疗带来新的曙光。

补体因子H与老年性黄斑变性

原发性视网膜色素变性患者红细胞免疫功能的检测

阵发性睡眠性血红蛋白尿症的靶向治疗

阵发性睡眠性血红蛋白尿症（PNH）是一种罕见的造血干细胞克隆性的溶血性疾病，目前治疗困难。重组人源型抗补体蛋白C5单克隆抗体（eculizumab）的出现及临床应用开启了PNH靶向治疗的新篇章。eculizumab通过与补体C5结合，防止C5裂解形成C5b及膜攻击复合物，减少溶血，降低输血需求，提高患者生活质量。2007年，eculizumab分别被美国食品和药物管理局、欧盟委员会通过用于治疗PNH血管内溶血。现对PNH患者使用eculizumab靶向治疗作一综述。